Polyphasic characterization of “Aspergillus nidulans var. roseus” ATCC 58397

Tóth, Viktória; Nagy, Csilla Terézia; Miskei, Márton; Pócsi, István; Emri, Tamás

doi:10.1007/s12223-011-0059-4

Cited by 17 publications

(23 citation statements)

References 20 publications

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“…roseus was low at 24°C when it was compared to those measured with A. nidulans var. roseus at 37°C or even to those measured with A. nidulans FGSC A4 at 24°C (Tóth et al 2011). To sum it up, the acquired ECB tolerance was explained by several factors in A. nidulans var.…”

Section: Discussionmentioning

confidence: 83%

“…roseus ATCC 58397 has a great industrial importance and, depending on the culturing conditions, it produces ECB even at as high as 500 mg l −1 concentration at 24°C; however, at 37°C no ECB production has been observed (Boeck and Kastner 1981;Tóth et al 2011). Considering its ECB production potential, it is reasonable to assume that A. nidulans var.…”

Section: Discussionmentioning

confidence: 99%

“…It is worth mentioning that recent studies demonstrated that this isolate is not an A. nidulans strain but belongs to the Emericella rugulosa species. However, it has some properties which are not typical for the E. rugulosa strains either (Klich et al 2001;Tóth et al 2011). In this article, we therefore kept the original and widely used species name for clarity.…”

Section: Strainsmentioning

confidence: 99%

“…To amplify selected DNA sequences, rRT-PCR primer pairs (Table 1) were used. PCR amplification, cloning, and sequencing were performed as described elsewhere (Tóth et al 2011). All sequences were deposited at the GenBank under the accession numbers JN862788-JN862796 and/or presented in Supplementary Table 1.…”

Section: Dna Sequencingmentioning

confidence: 99%

“…Because Tóth et al (2011) demonstrated that A. nidulans var. roseus ATCC 58397 does not belong to the A. nidulans species gene (mRNA)-specific sequences amplified in rRT-PCR assays were also sequenced after PCR amplification and cloning.…”

Section: Ecb Altered the Cell Wall Composition In A Nidulans Var Romentioning

confidence: 99%

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The echinocandin B producer fungus Aspergillus nidulans var. roseus ATCC 58397 does not possess innate resistance against its lipopeptide antimycotic

Tóth

Nagy

Pócsi

et al. 2012

Appl Microbiol Biotechnol

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Aspergillus nidulans var. roseus ATCC 58397 is an echinocandin B (ECB) producer ascomycete with great industrial importance. As demonstrated by ECB/caspofungin sensitivity assays, A. nidulans var. roseus does not possess any inherent resistance to echinocandins, and its tolerance to these lipopeptide antimycotics are even lower than those of the non-producer A. nidulans FGSC A4 strain. Under ECB producing conditions or ECB exposures, A. nidulans var. roseus induced its ECB tolerance via up-regulating elements of the chitin biosynthetic machinery and, hence, through changing dynamically the composition of its own cell wall. Importantly, although the specific β-1,3-glucan synthase activity was elevated, these changes reduced the β-glucan content of hyphae considerably, but the expression of fksA, encoding the catalytic subunit of β-1,3-glucan synthase, the putative target of echinocandins in the aspergilli, was not affected. These data suggest that compensatory chitin biosynthesis is the centerpiece of the induced ECB tolerance of A. nidulans var. roseus. It is important to note that the induced tolerance to ECB (although resulted in paradoxical growth at higher ECB concentrations) was accompanied with reduced growth rate and, under certain conditions, even sensitized the fungus to other stress-generating agents like SDS. We hypothesize that although ECB-resistant mutants may arise in vivo in A. nidulans var. roseus cultures, their widespread propagation is severely restricted by the disadvantageous physiological effects of such mutations.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Strainsmentioning

confidence: 99%

Section: Dna Sequencingmentioning

confidence: 99%

Section: Ecb Altered the Cell Wall Composition In A Nidulans Var Romentioning

confidence: 99%

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