Polypeptides of the tail fibres of bacteriophage T4

King, Jonathan; Laemmli, Ulrich Karl

doi:10.1016/0022-2836(71)90148-3

Cited by 923 publications

(298 citation statements)

References 34 publications

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“…The preparation of antisera against rat CYZM and ~uIAGP and the immunoprecipitations have been described [33,34]. For the quantification of the radioactivity incorporated into (YAM, the respective bands identified by fluorography [35] were cut from the SDS-PAGE gels [36], solubilized with protosol/water (9: 1, v/v) at 45°C overnight and counted in a liquid scintillation spectrometer.…”

Section: Methodsmentioning

confidence: 99%

Induction of rat α₂‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor

Bauer

Birmelin

Northoff³

et al. 1984

FEBS Letters

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Turpentine injection into rats elicits enhanced secretion of acute phase proteins inclucing cr,-macroglobulin (a2M). Hypophysectomized rats, however, do not respond in this way unless dexamethasone is given together with turpentine. On the other hand, dexamethasone injection alone did not result in an induction of cr,M synthesis. When a medium of Kupffer cell cultures was added to hepatocytes, a dose-dependent stimulation of u,M synthesis of up to 4-fold after l&l2 h was observed. However, the presence of low concentrations ( 10m9 M) of dexamethasone was essential for the stimulatory effect. We conclude that the acute phase induction of u,M in hepatocytes requires the synergistic action of glucocorticoids and a non-dialysable factor secreted by Kupffer cells.

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Section: Methodsmentioning

confidence: 99%

Induction of rat α₂‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor

Bauer

Birmelin

Northoff³

et al. 1984

FEBS Letters

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“…Thin cartilage chips (1-5 mg) from either sacral or ilial cartilage were directly digested with cyanogen bromide (CB) as described by Scott and Veis (17). The resulting fragments (CB-peptides) were separated on 15% SDS-polyacrylamide gels according to the method of King and Laemmli (14). The gels were stained with 0.25% (weight/volume) Coomassie blue in 20% (wh) trichloracetic acid in water.…”

Section: Methodsmentioning

confidence: 99%

Biochemical and morphologic studies of cartilage from the adult human sacroiliac joint

Paquin

Rest

Marie

et al. 1983

Arthritis & Rheumatism

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The cartilage of the adult sacroiliac joint contains type I1 collagen and a high concentration of glycosaminoglycan. Furthermore, large aggregating proteoglycans and link proteins were extracted from the sacral cartilage. In these respects the sacroiliac cartilage is similar to that in the peripheral joints. However, while the organization of the collagen in the sacral cartilage is typical of an articular cartilage, the organization of the ilia1 cartilage is very different, and throughout its depth it possesses narrow collagen fibrils arranged parallel to the articular surface.The sacroiliac joint can be involved in a wide variety of pathologic processes, ranging from diseases which affect predominantly the peripheral diarthrodial joints, e.g., rheumatoid arthritis, to the enthesopathies From the Joint Diseases

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“…SDSjPAGE was performed in a 12.5% polyacrylamide slab gel with a Tris/glycine buffer system, as described by King and Laemmli [9]. The relative molecular mass of the subunit was determined from its mobility relative to those of the standard proteins.…”

Section: Sds/polyacrylamide Gel Electrophoresismentioning

confidence: 99%

Purification and characterization of a novel FMN‐dependent enzyme

Kataoka

Shimizu

Yamada

1992

European Journal of Biochemistry

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Membrane-bound L-(+)-pantoy1 lactone dehydrogenase, an enzyme that catalyzes the formation of ketopantoyl lactone from L-(+)-pantoy1 lactone, was solubilized with Brij 35 and purified 78-fold to apparent homogeneity, with a 3.7% overall recovery, from Nocardia asteroides through purification procedures including successive ammonium sulfate fractionation, and DEAE-Sephacel, Sepharose CL-6B and Cellulofine GC-700-m column chromatography in the presence of Brij 35. The relative molecular mass of the native enzyme, as estimated on high-performance gel-permeation chromatography, is at least more than 600 kDa and its subunit molecular mass is 42 kDa. The enzyme shows high specificity for L-(+)-pantoy1 lactone as a substrate ( K , = 26.8 mM; V,,, = 4.22 pmol . min-' . mg protein-'). Brij 35 acts as a stabilizer and also as an efficient activator of the enzyme. The prosthetic group of L-(+)-pantoy1 lactone dehydrogenase was identified as noncovalently bound FMN In previous papers [l -31, we reported that the stereoselective oxidation of L-(+)-pantoy1 lactone to ketopantoyl lactone (Scheme 1) in a racemic mixture was a promising reaction for the practical production of D-( -)-pantoyl lactone, which is an important intermediate for the chemical synthesis of D-(+)-pantothenic acid. It was found that coryneform bacteria, e. g. Nocardiu, Rhodococcus and Corynehactrrium, exhibit high ability and specificity for the oxidation of L-(+)-pantoyl lactone to ketopantoyl lactone [l] and that 1,2-propanediol markedly enhanced the oxidizing activity of such microorganisms [l]. While the L-(+)-pantoyllactone-oxidizing reaction is quite useful for industrial applications, the enzyme catalyzing this stereoselective oxidation has not been identified. The enzyme might be concerned with the metabolism of 1,2-propanediol, because it is induced by 1,2-propanediol. Thus, the characterization of the enzyme seemed to be necessary for clarification of the metabolism of 3 ,Zpropanediol in microorganisms.In the present paper, we report on the purification and characterization of L-(+)-pantoy1 lactone dehydrogenase from N . asteroides.

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Polypeptides of the tail fibres of bacteriophage T4

Cited by 923 publications

References 34 publications

Induction of rat α₂‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor

Induction of rat α₂‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor

Biochemical and morphologic studies of cartilage from the adult human sacroiliac joint

Purification and characterization of a novel FMN‐dependent enzyme

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Polypeptides of the tail fibres of bacteriophage T4

Cited by 923 publications

References 34 publications

Induction of rat α2‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor

Induction of rat α2‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor

Biochemical and morphologic studies of cartilage from the adult human sacroiliac joint

Purification and characterization of a novel FMN‐dependent enzyme

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Product

Resources

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Induction of rat α₂‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor

Induction of rat α₂‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor