Polypeptide composition of a Photosystem II core complex. Presence of a herbicide-binding protein

Isolation of photosystem II reaction center complex by affinity chromatography with the peripheral 33‐kDa polypeptide as ligand

“…1). The absorption spectrum of the preparation was characteristic of the PS II reaction center complexes previously reported [4,16] (fig.2). The Chl a/b ratio of the preparation obtained by this procedure was always higher than 8.0.…”

“…the 47-, 43-, 32-, 30-and 8-kDa proteins, can be assigned to CP47, CP43, D1, D2 and cytochrome b-559 [2,3]. The polypeptide composition was identical with the preparation reported in [16]. Recently, the PS II reaction center complexes, which retained the 33-kDa polypeptide and oxygen-evolution activity, were prepared from thylakoids using digitonin [4] and from PS II particles using octylglucoside [5,6].…”

THE 32000 DALTON QB PROTEIN OF PHOTOSYSTEM II

“…The increased resolution of using lithium dodecyl sulfate (LDS) over sodium dodecyl sulfate (SDS) in the gel system also aids in the separation of these two proteins. However, following removal of the lysine-rich protein and subsequent electrophoresis in LDS and 4M urea another poorly-staining diffuse band referred to as D2 (Delapelaire, 1984) was identified in the PS I1 core complex (Satoh et al, 1983). The identification of this third PS I1 polypeptide with M, = 32000 will be discussed in Section 6 .…”

ATP‐dependent protein synthesis in isolated pea chloroplasts

“…This protein is also known to be one of the most rapidly metabolizing components in illuminated chloroplasts [3,4]. The lightdependent synthesis of this protein is now understood to represent the reparative process of photodamaged reaction centers [5].…”