Polymorphisms of the prion protein gene (PRNP) in Hanwoo (Bos taurus coreanae) and Holstein cattle

Jeong, Byung‐Hoon; Sohn, Hyun‐Joo; Lee, Jong-O; Kim, Nam-Ho; Kim, Jae-Il; Lee, Seyoung; Cho, In‐Soo; Joo, Yi-Seok; Carp, Richard I.; Kim, Yong‐Sun

doi:10.1266/ggs.80.303

Cited by 26 publications

(21 citation statements)

References 31 publications

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“…1993). We found similarities between Japanese Holstein and Korean Holstein, and between JBr and Korean native Hanwoo for octapeptide repeat frequency (Jeong et al. 2005).…”

Section: Discussionmentioning

confidence: 78%

Gene and haplotype polymorphisms of the Prion gene (PRNP) in Japanese Brown, Japanese native and Holstein cattle

Msalya

Shimogiri²,

Okamoto³

et al. 2009

Animal Science Journal

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Polymorphisms in the prion protein gene (PRNP) are known to be associated with transmissible spongiform encephalopathies in human, sheep and goats. There is tentative association between PRNP promoter polymorphism and bovine spongiform encephalopathy (BSE) susceptibility in cattle. In this study, we genotyped for six bovine PRNP polymorphic sites including a 23-bp indel in the promoter, a 12-bp indel in the intron 1, two nonsynonymous single nucleotide polymorphisms (SNPs), octapeptide repeats in the coding region and a 14-bp indel in the 3'-untranslated region in 178 animals representing Japanese Brown, Kuchinoshima feral, Mishima, Japanese Shorthorn and Holstein. In 64 Japanese Brown cattle, three indel sites were polymorphic. All of the six sites were monomorphic in Kuchinoshima. The 23-bp and 12-bp indel sites were polymorphic in Mishima cattle. The 23-bp and 14-bp indel sites were polymorphic in Japanese Shorthorn cattle. Both SNP sites were monomorphic in all cattle examined in this study. At the 23-bp indel site, the genotype frequencies of Japanese Brown and Holstein breeds were similar to that of BSE affected cattle. We estimated 12 different haplotypes from these genotypic data. A '23-12-K6S14+' haplotype was the major haplotype in all populations, whose frequencies ranged from 0.50 to 1.00.

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“…1993). We found similarities between Japanese Holstein and Korean Holstein, and between JBr and Korean native Hanwoo for octapeptide repeat frequency (Jeong et al. 2005).…”

Section: Discussionmentioning

confidence: 78%

Gene and haplotype polymorphisms of the Prion gene (PRNP) in Japanese Brown, Japanese native and Holstein cattle

Msalya

Shimogiri²,

Okamoto³

et al. 2009

Animal Science Journal

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“…In previous studies of the PRNP gene in Hanwoo cattle, only a few genetic variations in the PRNP coding region were reported. Jeong et al [15] analyzed the open reading frame (ORF) region of the PRNP gene in 120 Hanwoo cattle and found two polymorphisms: the third position of codon 78 (G234A) and the third position of codon 192 (C576T). However, these authors did not find any variation in the octapeptide repeat.…”

Section: Discussionmentioning

confidence: 99%

“…To date, no BSE cases have been reported in Hanwoo. Genotypic descriptions of BSE-related genetic factors, such as SNPs or the number of octapeptide repeats in PRNP , have rarely been reported for Hanwoo, and the genetic frequencies and features related to BSE susceptibility have been observed in only a limited number of samples [14,15]. In the present study, we performed a detailed analysis of both sequence variations in the coding region of PRNP and prevalent indel polymorphisms in the non-coding region of PRNP using a large number of samples from Hanwoo cattle.…”

Section: Introductionmentioning

confidence: 99%

Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle

2012

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“…Polymorphisms in the PrP gene ORF have been detected in many cattle breeds [38,40,42,[54][55][56][57][58][59][60][61][62][63][64]. Several breeds have the same polymorphisms.…”

Section: Prp Gene Polymorphisms and Their Regulation Of Prp Expressionmentioning

confidence: 99%

Structure of the Prion Protein and Its Gene: An Analysis Using Bioinformatics and Computer Simulation

Sakudo¹,

Gao²,

Kawashita³

et al. 2010

CPPS

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Prion protein (PrP) gene encodes cellular PrP (PrPC), a glycosylphosphatidylinositol (GPI)-anchored cell membrane protein indispensable for infections of prion, which causes Creutzfeldt-Jakob disease (CJD) in humans, bovine spongiform encephalopathy (BSE) in cattle, and scrapie in sheep. Although PrPC is known to be converted into an abnormal isoform (PrPSc) upon prion infection and play an important role in prion diseases, the mechanisms involved remain unclear, partly due to the insolubility of PrPSc, which prevents experimental biochemical and biophysical analyses. Recently, with improvements in computer power and methods, computer analyses have been contributing more to prion studies. A comparison of PrP gene sequences revealed mutations and polymorphisms in the open reading frame (ORF) of the human PrP gene related to prion diseases. In contrast, little mutations or polymorphisms related to susceptibility to BSE were found in the ORF of the bovine PrP gene, though relationships between insertion/deletion (Ins/Del) polymorphisms of the PrP gene promoter and susceptibility to BSE have been found. Our results have shown that the specific protein 1 (Sp1) plays important role in the activity of PrP gene promoter, which is influenced by polymorphisms in the Sp1 binding sites. The potential structural dynamics of PrP have been simulated by computational methods such as molecular dynamics (MD) and quantum mechanics (QM). The proposed mechanisms of conversion have revealed new insights in prion diseases. In this review, we will introduce the gene structure, polymorphisms, and potential structural dynamics of PrP revealed by basic and advanced computational analyses. The possible contribution of these methods to elucidation of the pathogenicity of prion diseases and functions of PrPC is discussed.

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Polymorphisms of the prion protein gene (PRNP) in Hanwoo (Bos taurus coreanae) and Holstein cattle

Cited by 26 publications

References 31 publications

Gene and haplotype polymorphisms of the Prion gene (PRNP) in Japanese Brown, Japanese native and Holstein cattle

Gene and haplotype polymorphisms of the Prion gene (PRNP) in Japanese Brown, Japanese native and Holstein cattle

Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle

Structure of the Prion Protein and Its Gene: An Analysis Using Bioinformatics and Computer Simulation

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