Polymorphisms in the LASP1 gene allow selection for smaller stature in ponies

Bartholazzi, Aylton; Quirino, C. R.; Vega, Wilder Hernando Ortiz; Rua, Miguel Alejandro Silva; David, Caroline Marçal Gomes; Jardim, Júlia Gazzoni

doi:10.1016/j.livsci.2018.07.015

Cited by 6 publications

(2 citation statements)

References 24 publications

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“…As a core transcription factor in embryonic stem cells, ZNF281 (zinc finger protein 281) was found to be related to spontaneous osteochondrogenic differentiation [38]. Other studies showed the strong association of LCORL/NCAPG, HMGA2, PROP1, LASP [39], ZFAT, DIAPH3 [40], ACTN2, ADAMTS17, GH1, ANKRD1 [40], and ACAN [41,42] with miniature size and dwarfism in a variety of pony breeds, including Shetland [41,43], miniature [44], Welsh ponies [45], German warmblood horses [46], American miniature horses, Brazilian ponies [47], and Jeju ponies [48], as well as B4GALT7 [49] and PROP1 [50] in Friesian horses. These genes were not found in this study, which may be due to the expression specificity of lncRNAs in different horse breeds [27] and different omics levels, since TBX3, under strong selection in Chinese ponies, was also identified in this study.…”

Section: Discussionmentioning

confidence: 97%

Identification of Novel lncRNAs Differentially Expressed in Placentas of Chinese Ningqiang Pony and Yili Horse Breeds

Yun

Zhang

et al. 2020

Animals

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As a nutrient sensor, the placenta plays a key role in regulating fetus growth and development. Long non-coding RNAs (lncRNAs) have been shown to regulate growth-related traits. However, the biological function of lncRNAs in horse placentas remains unclear. To compare the expression patterns of lncRNAs in the placentas of the Chinese Ningqiang (NQ) and Yili (YL) breeds, we performed a transcriptome analysis using RNA sequencing (RNA-seq) technology. NQ is a pony breed with an average adult height at the withers of less than 106 cm, whereas that of YL is around 148 cm. Based on 813 million high-quality reads and stringent quality control procedures, 3011 transcripts coding for 1464 placental lncRNAs were identified and mapped to the horse reference genome. We found 107 differentially expressed lncRNAs (DELs) between NQ and YL, including 68 up-regulated and 39 down-regulated DELs in YL. Six (TBX3, CACNA1F, EDN3, KAT5, ZNF281, TMED2, and TGFB1) out of the 233 genes targeted by DELs were identified as being involved in limb development, skeletal myoblast differentiation, and embryo development. Two DELs were predicted to target the TBX3 gene, which was found to be under strong selection and associated with small body size in the Chinese Debao pony breed. This finding suggests the potential functional significance of placental lncRNAs in regulating horse body size.

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Section: Discussionmentioning

confidence: 97%

Identification of Novel lncRNAs Differentially Expressed in Placentas of Chinese Ningqiang Pony and Yili Horse Breeds

Yun

Zhang

et al. 2020

Animals

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“…The ligand-dependent nuclear receptor co-repressor like protein ( LCORL ) is associated with body size in several horse breeds 18,55–57 . Moreover, a recent study has shown that a T homozygous genotype on rs68603064 at ECA 3 near LCORL is significantly associated with lower wither height in Brazilian pony, with average wither height less than 158 cm 58 . In this study, we observed the same SNP to be fixed in the JH, which shows an average wither height of 122 cm.…”

Section: Resultsmentioning

confidence: 97%

Comprehensive genome and transcriptome analyses reveal genetic relationship, selection signature, and transcriptome landscape of small-sized Korean native Jeju horse

Srikanth

Kim

Park

et al. 2019

Sci Rep

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The Jeju horse, indigenous to the Jeju Island in Korea may have originated from Mongolian horses. Adaptations to the local harsh environment have conferred Jeju horse with unique traits such as small-sized body, stocky head, and shorter limbs. These characteristics have not been studied previously at the genomic level. Therefore, we sequenced and compared the genome of 41 horses belonging to 6 breeds. We identified numerous breed-specific non-synonymous SNPs and loss-of-function mutants. Demographic and admixture analyses showed that, though Jeju horse is genetically the closest to the Mongolian breeds, its genetic ancestry is independent of that of the Mongolian breeds. Genome wide selection signature analysis revealed that genes such as LCORL, MSTN, HMGA2, ZFAT, LASP1, PDK4, and ACTN2, were positively selected in the Jeju horse. RNAseq analysis showed that several of these genes were also differentially expressed in Jeju horse compared to Thoroughbred horse. Comparative muscle fiber analysis showed that, the type I muscle fibre content was substantially higher in Jeju horse compared to Thoroughbred horse. Our results provide insights about the selection of complex phenotypic traits in the small-sized Jeju horse and the novel SNPs identified will aid in designing high-density SNP chip for studying other native horse breeds.

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Candidate genes for height measurements in Campolina horses

et al. 2023

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Context Genomic tools allow for increased efficiency in animal production and, consequently, greater production gains. Morphometric measurements of height can be used for breed characterisation/registration, and sex distinction, in addition to being associated with balance, posture, and the range of motion of the limbs, influencing the dynamics and quality of the gait. Aims This study was conducted to examine candidate genes related to the morphometric measurement of heights in Campolina horses. Methods The study involved information from 18 876 horses of this breed, containing phenotypic data of the measurements of withers, croup, back, and barrel heights, provided by the Brazilian Association of Campolina Horse Breeders (ABCCCampolina). Of these, 48 horses of the Brazilian Campolina breed were genotyped with the Equine 65 K lllumina® chip. The effects of the single-nucleotide polymorphisms were estimated by the weighted single-step method, using BLUPF90 family programs. Genes in the main chromosomal regions were identified by consultation with the NCBI. Key results A genomic-wide association study for withers height showed that 46% of the variation in this measurement was explained by 20 genomic windows. For croup height, 19 genomic windows were responsible for explaining 44% of the variation. Sixteen genomic windows were responsible for explaining 37.8% of the variation in back height and 18 genomic regions explained 39.7% of the variation in barrel height. Conclusion The ADAM17, SOX11, and KLF11 genes are located in the genomic windows that most explain the variations in the traits under study and have a biological action on the locomotor system. Implications The ADAM17, SOX11, and KLF11 genes are candidates for fine mapping and construction of molecular markers.

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Polymorphisms in the LASP1 gene allow selection for smaller stature in ponies

Cited by 6 publications

References 24 publications

Identification of Novel lncRNAs Differentially Expressed in Placentas of Chinese Ningqiang Pony and Yili Horse Breeds

Identification of Novel lncRNAs Differentially Expressed in Placentas of Chinese Ningqiang Pony and Yili Horse Breeds

Comprehensive genome and transcriptome analyses reveal genetic relationship, selection signature, and transcriptome landscape of small-sized Korean native Jeju horse

Candidate genes for height measurements in Campolina horses

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