Polymorphisms in FADS1 and FADS2 alter desaturase activity in young Caucasian and Asian adults

Merino, Diana M.; Johnston, Holly M.; Clarke, Shannon; Roke, Kaitlin; Nielsen, Daiva E.; Badawi, Alaa; El-Sohemy, Ahmed; W.L., David; Mutch, David M.

doi:10.1016/j.ymgme.2011.02.012

Cited by 119 publications

(132 citation statements)

References 33 publications

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“…The expression was decreased in SAT ( P = 0.035) and PBMCs ( P = 2.25 × 10 FADS2 genes associate with serum FA profi le, as published before (14)(15)(16)(17), and with IL-1 ␤ expression in AT. The SNPs, rs174616 ( FADS2 ) and rs174547 ( FADS1 ), were in Hardy-Weinberg equilibrium (0.277, 0.591), and in linkage disequilibrium (r 2 = 0.33, D'= 0.70), as known due the existence of these genes in the same gene cluster ( 17 ).…”

Section: Il-1 ␤ Expression Is Decreased After Surgery-induced Weight mentioning

confidence: 55%

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FADS2 genotype regulates delta-6 desaturase activity and inflammation in human adipose tissue

Vaittinen

Walle

Kuosmanen

et al. 2016

Journal of Lipid Research

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Section: Il-1 ␤ Expression Is Decreased After Surgery-induced Weight mentioning

confidence: 55%

“…These SNPs were chosen for the current analysis because of their previous association with PUFA levels and desaturase activities (14)(15)(16)(17).…”

Section: Genotype Analysesmentioning

confidence: 99%

FADS2 genotype regulates delta-6 desaturase activity and inflammation in human adipose tissue

Vaittinen

Walle

Kuosmanen

et al. 2016

Journal of Lipid Research

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“…At baseline, we found several highly signifi cant SNP minor allele associations with increased proportions of substrates and decreased proportions of products in plasma and a strong association with increased DGLA in erythrocytes. Previous studies have shown association of several SNPs in this region with LC-PUFAs in plasma ( 4,(9)(10)(11)(12)(13)(14) and erythrocyte membranes ( 4,8,(10)(11)(12)15 ), although most did not fi nd associations with proportions of Fig. 3.…”

Section: Snp Associations With Plasma and Erythrocyte Variables Aftermentioning

confidence: 82%

Genetic variation at the FADS1-FADS2 gene locus influences delta-5 desaturase activity and LC-PUFA proportions after fish oil supplement

Al-Hilal

AlSaleh

Maniou

et al. 2013

Journal of Lipid Research

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This article is available online at http://www.jlr.org infl ammatory eicosanoids ( 1 ). The major dietary LC-PUFA is linoleic acid (LA,, with lower amounts of n-3 fatty acids, chiefl y ␣ -linolenic acid (ALA, 18:3n-3), eicosapentaenoic acid (EPA,, and docosahexaenoic acid (DHA,. The composition of fatty acids in tissues refl ects both dietary fat ( 2 ) and the effi ciency of elongation and desaturation of their dietary precursors, the two essential fatty acids LA and ALA ( Fig. 1 ).Delta-5 and delta-6 desaturases (D5D and D6D) are the key enzymes in endogenous desaturation ( 3, 4 ), expressed at high levels in liver, brain, heart, and lung ( 5, 6 ). D5D and D6D are encoded, respectively, by the FADS1 and FADS2 genes, which lie in a region of strong linkage disequilibrium (LD) on chromosome 11. Several single nucleotide polymorphisms (SNP) in the FADS1-FADS2 cluster have been shown to be associated with plasma or erythrocyte phosphoglyceride LC-PUFA concentrations in genome-wide association (GWA) studies ( 7,8 ). Associations of SNPs and haplotypes in this region have been shown with fatty acids in plasma ( 4, 9-14 ), erythrocyte membranes ( 4,8,(10)(11)(12)15 ), and adipose tissue ( 16 ). Surrogate estimates of desaturase activity based on ratios of product:substrate ( 15, 17 ) suggest lower levels are associated with the minor alleles of SNPs in the FADS1-FADS2 LD block. GWA studies have also identifi ed several loci in the FADS gene cluster, at which minor alleles were associated with lower total cholesterol ( 18 )

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“…Further-more, arachidonic acid (C20:4) was found to be the major metabolite of dietary LA (C18:2) (Poureslami et al, 2010). Merino et al (2011) reported that genetic variation in the FADS gene cluster could alter desaturase activity in human subjects of Caucasian and Asian descent. In the present study, the data was insufficient to determine whether the two adjacent mutations could result in enzyme activity; therefore, the mechanism that caused the observed changes in fatty acid content require further validation.…”

Section: Discussionmentioning

confidence: 99%

Adjacent SNPs in the transcriptional regulatory region of the FADS2 gene associated with fatty acid and growth traits in chickens

Zhu¹,

Tian²,

Zhang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Delta-6 fatty acid desaturases are rate-limiting desaturases involved in metabolic processes of fatty acids, and they are encoded by the FADS2 gene. In the current study, an F 2 resource population of Gushi chickens crossed with Anak broilers was used to investigate the genetic effects of the chicken FADS2. Two adjacent single nucleotide polymorphisms (SNPs) (g.4290C>G and g.4291C>A) were identified in the transcriptional regulatory region of the FADS2 gene by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and created restriction site-PCR-RFLP. Associations between the two SNPs with chicken fatty acid contents and growth traits were determined using linkage disequilibrium, haplotype construction, and association analysis. The two SNPs and their haplotype combinations were significantly associated with linoleic

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Polymorphisms in FADS1 and FADS2 alter desaturase activity in young Caucasian and Asian adults

Cited by 119 publications

References 33 publications

FADS2 genotype regulates delta-6 desaturase activity and inflammation in human adipose tissue

FADS2 genotype regulates delta-6 desaturase activity and inflammation in human adipose tissue

Genetic variation at the FADS1-FADS2 gene locus influences delta-5 desaturase activity and LC-PUFA proportions after fish oil supplement

Adjacent SNPs in the transcriptional regulatory region of the FADS2 gene associated with fatty acid and growth traits in chickens

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