Polymorphism analysis in genes of the somatotropic axis in Nellore cattle selected for growth

Cardoso, Diércles Francisco; Souza, Fernanda Fernandes; Camargo, Gregório Miguel Ferreira de; Fonseca, Patrícia Dias da Silva; Fonseca, Larissa Fernanda Simielli; Braz, Camila Urbano; Boligon, Arione Augusti; Mercadante, Maria Eugênia Zerlotti; Albuquerque, Lúcia Galvão de; Tonhati, Humberto

doi:10.1016/j.gene.2014.05.033

Cited by 10 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Nellore experimental breeding program from IZ has gained national recognition and contributed substantially to the field of beef cattle breeding and genetics. The differential selection among the three selection lines has enabled in-depth studies of weight-related traits and feed efficiency, providing essential insights into the genetic information of livestock (e.g., Ayres et al, 2010 ; Cardoso et al, 2014 ; Cardoso et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Detection and characterization of copy number variation in three differentially-selected Nellore cattle populations

Benfica,

Brito,

do Bem

et al. 2024

Front. Genet.

View full text Add to dashboard Cite

Introduction: Nellore cattle (Bos taurus indicus) is the main beef cattle breed raised in Brazil. This breed is well adapted to tropical conditions and, more recently, has experienced intensive genetic selection for multiple performance traits. Over the past 43 years, an experimental breeding program has been developed in the Institute of Animal Science (IZ, Sertaozinho, SP, Brazil), which resulted in three differentially-selected lines known as Nellore Control (NeC), Nellore Selection (NeS), and Nellore Traditional (NeT). The primary goal of this selection experiment was to determine the response to selection for yearling weight (YW) and residual feed intake (RFI) on Nellore cattle. The main objectives of this study were to: 1) identify copy number variation (CNVs) in Nellore cattle from three selection lines; 2) identify and characterize CNV regions (CNVR) on these three lines; and 3) perform functional enrichment analyses of the CNVR identified.Results: A total of 14,914 unique CNVs and 1,884 CNVRs were identified when considering all lines as a single population. The CNVRs were non-uniformly distributed across the chromosomes of the three selection lines included in the study. The NeT line had the highest number of CNVRs (n = 1,493), followed by the NeS (n = 823) and NeC (n = 482) lines. The CNVRs covered 23,449,890 bp (0.94%), 40,175,556 bp (1.61%), and 63,212,273 bp (2.54%) of the genome of the NeC, NeS, and NeT lines, respectively. Two CNVRs were commonly identified between the three lines, and six, two, and four exclusive regions were identified for NeC, NeS, and NeT, respectively. All the exclusive regions overlap with important genes, such as SMARCD3, SLC15A1, and MAPK1. Key biological processes associated with the candidate genes were identified, including pathways related to growth and metabolism.Conclusion: This study revealed large variability in CNVs and CNVRs across three Nellore lines differentially selected for YW and RFI. Gene annotation and gene ontology analyses of the exclusive CNVRs to each line revealed specific genes and biological processes involved in the expression of growth and feed efficiency traits. These findings contribute to the understanding of the genetic mechanisms underlying the phenotypic differences among the three Nellore selection lines.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection and characterization of copy number variation in three differentially-selected Nellore cattle populations

Benfica,

Brito,

do Bem

et al. 2024

Front. Genet.

View full text Add to dashboard Cite

show abstract

“…Due to the limited number of sample (N=15 each group), no statistical analysis was performed. Similarly Beauchemin et al (2006) has reported that effects of MspI polymorphism were not found in Brahman cattle and Nellore cattle population (Cardoso et al, 2014). On the contrary, Unanian et al (2000) reported that MspI polymorphism effects on the growth traits in Nellore cattle population and describes a significant effect on the average daily gain (ADG) from weaning at 15 months with DD genotype (equivalent with MspI-/-) and weight at first estrus and first calving in heifers in Holstein cattle (Arango et al, 2014).…”

Section: Genotype Analysis Of Gh Gene Inmentioning

confidence: 92%

Genotype analysis of partial growth hormone gene (GH891│MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle

Hartatik

Putra

Volkandari

et al. 2018

J. Indonesian Trop. Anim. Agric.

View full text Add to dashboard Cite

ABSTRAKGen Growth Hormone (GH) merupakan sebuah gen yang mengontrol sintesis hormon pertumbuhan dan berperan dalam metabolisme tubuh. Identifikasi polimorfisme suatu gen penting dilakukan untuk memperoleh informasi awal dalam mengetahui penanda genetik yang berhubungan dengan sifat-sifat ekonomis yang diinginkan. Pemetaan genetik sangat penting dalam proses seleksi. Sapi Pesisir adalah salah satu sapi lokal Indonesia yang ditemukan di Sumatera Barat dan sangat adatif terhadap lingkungan setempat. Penelitian ini bertujuan untuk mengidentifikasi genotip berdasarkan sebagian gen Growth Hormone (GH891│MspI) pada sapi Pesisir dan sapi Persilangan Simmental-Pesisir (SimPes) dan hubungannya dengan sifat pertumbuhan (ukuran tubuh dan berat badan). Sebanyak 30 sampel darah sapi yang terdiri dari 15 Sapi Pesisir dan 15 Sapi SimPes digunakan dalam penelitian ini. Ukuran tubuh dan berat badan sapi diukur pada umur 12-18 bulan. Metode analisis yang digunakan adalah Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Hasil menunjukkan bahwa terdapat tiga (3) variasi genotip (MspI-/-, MspI+/-, MspI+/+) dengan frekuensi alel sebesar 53,3% untuk alel MspI-dan 46,7% untuk MspI+. Tidak ditemukan hubungan yang signifikan antara ukuran tubuh dan bobot badan kedua bangsa sapi tersebut berdasarkan ketiga genotip gen GH (GH891│MspI). Kesimpulan, terdapat tiga genotip gen GH pada populasi sapi Pesisir dan SimPes dan tidak berpengaruh signifikan terhadap performans pertumbuhan sapi.Kata Kunci: genotip, enzim MspI, gen Pertumbuhan, sifat pertumbuhan, sapi Pesisir, sapi SimPes ABSTRACTGrowth hormone gene that controls metabolism and body size of animals and polymorphism of the gene is known related to performance of livestock. Identification of gene polymorphisms was important to get early information to determine genetic markers associated with economically desirable traits. Genetic mapping is one of important characterizations in the selection process. Pesisir cattle is one of local cattle in Indonesia that are reared in West Sumatera and have become adaptive to local environment. The present study aimed to identify the genotype of Growth Hormone gene (GH891│MspI) of the Pesisir cattle and crossbred Simmental-Pesisir cattle (designated as SimPes) and relationship between growth performance (body weight and body size). The present study was Pesisir and Crossed SimPes Cattle (T. Hartatik et al.) 1 conducted to 30 blood samples consisted of 15 Pesisir cattle and 15 SimPes cattle. Body weight and body size were measured at 12-18 months of age. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method was used in this research. The results showed that there exist three variant genotypes (MspI-/-, MspI+/-, MspI+/+) and that allele frequencies of MspI-and MspI+ were 53.3% and 46.7%, respectively. Non-significant effect has found between GH's genotype and growth traits (Body measurement and body weight) in both of breed cattle. In conclusion, three variant genotypes (GH891│MspI) in Pes...

show abstract

“…Arango et al (2014) reported significant association (P o0.01) between GH markers and weight at first estrus and weight at first calving in Holstein heifers. Significant effect (P r0.05) between GH and longissimus muscle area was observed by Cardoso et al (2014) in Nellore animals from three different lines (selection, traditional, and control). Furthermore, these authors found significant effect (P r0.04) of the interaction of GH and the growth hormone receptor (GHR) with weight at 550 days of age.…”

Section: Tablementioning

confidence: 96%

“…According to Cardoso et al, (2014), PIT1 markers (named as POU1F1) were monomorphic in Nellore animals, which was not assigned to the allele fixation due to selection. Zhang et al (2009) found statistical difference (Po 0.05) between AA and AB alleles for body weight at 4 months of age in crossed Qinchuan-Germany Yellow animals.…”

Section: Tablementioning

confidence: 99%

Allele substitution effects of IGF1, GH and PIT1 markers on estimated breeding values for weight and reproduction traits in Canchim beef cattle

et al. 2015

View full text Add to dashboard Cite

a b s t r a c tThe aim of this study was to evaluate the allele substitution effects of IGF1, GH and PIT1 markers on the estimated breeding values (EBVs) for birth (BW) and weaning weights (WW), weight at 12 (W12) and 18 (W18) months of age, weight (WFC) and age (AFC) at first calving, and scrotal circumference measured at 12 (SC12) and 18 (SC18) months of age in Canchim beef cattle. Maternal effects were considered for birth (BW maternal ) and weaning (WW maternal ) weight. Regression analyses were carried out considering the EBVs, obtained from multi-trait analyses, and the deviations for each allele studied (four alleles for the IGF1 markers and two for GH and PIT1 markers, respectively). According to the results obtained for IGF1, the substitution effect of the "225" allele was significant (Pr 0.05) and presented higher EBVs for BW, BW maternal , WW, W12, W18, and SC18; and lower EBVs for WW maternal . The allele substitution effect observed for GH gene was significant (P r0.05) on EBVs for AFC and WFC. The "Valine" allele was responsible for lower EBVs for AFC and higher EBVs for WFC. For the PIT1 gene, the substitution effect of the "HinfÀ " allele was significant (Pr 0.05) on the EBVs for WW, WW maternal , AFC, and WFC, respectively. Important allele substitution effects were found for weight and reproduction traits in Canchim cattle. In general, markers on the IGF1 gene are related to higher EBVs for weight, while markers on the GH and PIT1 showed greater influence on the EBVs for age and weight at first calving. Adding molecular markers information on the selection process could result in increased genetic gains in Canchim cattle. Future studies using high-density genotyping platforms may contribute to our understanding of the effects of these genes on traits of economic importance in the Canchim breed. (D.P. Munari). Please cite this article as: Grossi, D.d.A., et al., Allele substitution effects of IGF1, GH and PIT1 markers on estimated breeding values for weight and reproduction traits in Canchim beef cattle. Livestock Science (2015), http://dx.

show abstract

Polymorphism analysis in genes of the somatotropic axis in Nellore cattle selected for growth

Cited by 10 publications

References 29 publications

Detection and characterization of copy number variation in three differentially-selected Nellore cattle populations

Detection and characterization of copy number variation in three differentially-selected Nellore cattle populations

Genotype analysis of partial growth hormone gene (GH891│MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle

Allele substitution effects of IGF1, GH and PIT1 markers on estimated breeding values for weight and reproduction traits in Canchim beef cattle

Contact Info

Product

Resources

About