Polymeric Nanoparticles as siRNA Drug Delivery System for Cancer Therapy: The Long Road to Therapeutic Efficiency

Frère, Antoine; Évrard, Brigitte; Mottet, Denis; Piel, Géraldine

doi:10.1016/b978-0-323-47347-7.00018-5

Cited by 7 publications

(6 citation statements)

References 150 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A large part of a solid tumor possesses a leaky vasculature, with enhanced permeability to increase the supply of nutrients and oxygen necessary for their rapid growth. AuNPs can pass through the fenestration in the blood vessel endothelium and accumulate in the tumor site [111,112]. To determine the pharmacokinetic profile and the half-life of the AuNPs, samples of the blood are usually collected during the testing period of the experiment, and the content of gold is quantitatively estimated.…”

Section: Genotoxicity and Mutagenicitymentioning

confidence: 99%

Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations

Kus‐Liśkiewicz

Fickers

Tahar

2021

IJMS

125

View full text Add to dashboard Cite

Recent advances in the synthesis of metal nanoparticles (MeNPs), and more specifically gold nanoparticles (AuNPs), have led to tremendous expansion of their potential applications in different fields, ranging from healthcare research to microelectronics and food packaging. The properties of functionalised MeNPs can be fine-tuned depending on their final application, and subsequently, these properties can strongly modulate their biological effects. In this review, we will firstly focus on the impact of MeNP characteristics (particularly of gold nanoparticles, AuNPs) such as shape, size, and aggregation on their biological activities. Moreover, we will detail different in vitro and in vivo assays to be performed when cytotoxicity and biocompatibility must be assessed. Due to the complex nature of nanomaterials, conflicting studies have led to different views on their safety, and it is clear that the definition of a standard biosafety label for AuNPs is difficult. In fact, AuNPs’ biocompatibility is strongly affected by the nanoparticles’ intrinsic characteristics, biological target, and methodology employed to evaluate their toxicity. In the last part of this review, the current legislation and requirements established by regulatory authorities, defining the main guidelines and standards to characterise new nanomaterials, will also be discussed, as this aspect has not been reviewed recently. It is clear that the lack of well-established safety regulations based on reliable, robust, and universal methodologies has hampered the development of MeNP applications in the healthcare field. Henceforth, the international community must make an effort to adopt specific and standard protocols for characterisation of these products.

show abstract

Section: Genotoxicity and Mutagenicitymentioning

confidence: 99%

Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations

Kus‐Liśkiewicz

Fickers

Tahar

2021

IJMS

125

View full text Add to dashboard Cite

show abstract

“…It has also been reported that polyplexes with neutral surface charge are more instable, aggregate more easily in solution and form precipitates [ 45 , 46 , 47 ]. NTS-polyplex NPs in PF25 showed a significant decrease in zeta potential as compared with NPs in EF25.…”

Section: Discussionmentioning

confidence: 99%

Development of a Parenteral Formulation of NTS-Polyplex Nanoparticles for Clinical Purpose

Aranda-Barradas¹,

Márquez²,

Quintanar³

et al. 2018

Pharmaceutics

View full text Add to dashboard Cite

Neurotensin (NTS)-polyplex is a nanoparticle system for targeted gene delivery that holds great promise for treatment of Parkinson’s disease and various types of cancer. However, the high instability in aqueous suspension of NTS-polyplex nanoparticles is a major limitation for their widespread clinical use. To overcome this obstacle, we developed a clinical formulation and a lyophilization process for NTS-polyplex nanoparticles. The reconstituted samples were compared with fresh preparations by using transmission electron microscopy, dynamic light scattering, electrophoretic mobility, circular dichroism and transfection assays in vitro and in vivo. Our formulation was able to confer lyoprotection and stability to these nanoparticles. In addition, transmission electron microscopy (TEM) and size exclusion-high performance liquid chromatography (SEC-HPLC) using a radioactive tag revealed that the interaction of reconstituted nanoparticles with fetal bovine or human serum did not alter their biophysical features. Furthermore, the formulation and the lyophilization procedure guaranteed functional NTS-polyplex nanoparticles for at least six months of storage at 25 °C and 60% relative humidity. Our results offer a pharmaceutical guide for formulation and long-term storage of NTS-polyplex nanoparticles that could be applied to other polyplexes.

show abstract

“…Specific attention should be paid to PS and LC of MeP when designing NPs [53,54]. NPs less than 800 nm accumulate in tumor tissues [33]. The level of LC affects the dose regime and the amount of drug administered [54].…”

Section: In Vivo Pharmacokinetic Studymentioning

confidence: 99%

“…The size and charge of NPs are critical parameters affecting their accumulation in the tissue of interest. Particle size plays a crucial role in the enhanced permeability and retention effect in tumor tissues, since the pore size of tumor vessels is in the range from 200 to 800 nm [33]. Moreover, the particle size must be more than 6 nm to escape kidney excretion or less than 500 nm to avoid internalization by macrophages [34].…”

Section: Introductionmentioning

confidence: 99%

Optimization, Characterization and Pharmacokinetic Study of Meso-Tetraphenylporphyrin Metal Complex-Loaded PLGA Nanoparticles

Mollaeva

Yabbarov

Sokół

et al. 2021

IJMS

View full text Add to dashboard Cite

The selection of technological parameters for nanoparticle formulation represents a complicated development phase. Therefore, the statistical analysis based on Box–Behnken methodology is widely used to optimize technological processes, including poly(lactic-co-glycolic acid) nanoparticle formulation. In this study, we applied a two-level three-factor design to optimize the preparation of nanoparticles loaded with cobalt (CoTPP), manganese (MnClTPP), and nickel (NiTPP) metalloporphyrins (MeP). The resulting nanoparticles were examined by dynamic light scattering, X-ray diffraction, Fourier transform infrared spectroscopy, MTT test, and hemolytic activity assay. The optimized model of nanoparticle formulation was validated, and the obtained nanoparticles possessed a spherical shape and physicochemical characteristics enabling them to deliver MeP in cancer cells. In vitro hemolysis assay revealed high safety of the formulated MeP-loaded nanoparticles. The MeP release demonstrated a biphasic profile and release mechanism via Fick diffusion, according to release exponent values. Formulated MeP-loaded nanoparticles revealed significant antitumor activity and ability to generate reactive oxygen species. MnClTPP- and CoTPP-nanoparticles specifically accumulated in tissues, preventing wide tissue distribution caused by long-term circulation of the hydrophobic drug. Our results suggest that MnClTPP- and CoTPP-nanoparticles represent the greatest potential for utilization in in anticancer therapy due to their effectiveness and safety.

show abstract

Polymeric Nanoparticles as siRNA Drug Delivery System for Cancer Therapy: The Long Road to Therapeutic Efficiency

Cited by 7 publications

References 150 publications

Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations

Biocompatibility and Cytotoxicity of Gold Nanoparticles: Recent Advances in Methodologies and Regulations

Development of a Parenteral Formulation of NTS-Polyplex Nanoparticles for Clinical Purpose

Optimization, Characterization and Pharmacokinetic Study of Meso-Tetraphenylporphyrin Metal Complex-Loaded PLGA Nanoparticles

Contact Info

Product

Resources

About