Polymeric microfluidic system for DNA analysis

Sun, Yi; Kwok, Yien Chian

doi:10.1016/j.aca.2005.09.035

Cited by 103 publications

(77 citation statements)

References 160 publications

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“…Because there are many beneficial size effects at the micro and nano scales, such as short mixing times, high efficiency in chemical reactions, and minute amounts of reagents and effluent liquids, micro/nano fluidic devices have been extensively studied for use in biomedical applications [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. In particular, nano fluidic devices that contain nano channels can be used for separation and filtration [21], single-molecule detection [22][23][24][25][26], highly efficient PCR [27], chemical analysis [28,29] and nano-photonic sensors [30], with great help of development of nanofabrication technologies [31][32][33][34][35][36][37][38][39][40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

Effects of Micromachining Processes on Electro-Osmotic Flow Mobility of Glass Surfaces

et al. 2013

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Silica glass is frequently used as a device material for micro/nano fluidic devices due to its excellent properties, such as transparency and chemical resistance. Wet etching by hydrofluoric acid and dry etching by neutral loop discharge (NLD) plasma etching are currently used to micromachine glass to form micro/nano fluidic channels. Electro-osmotic flow (EOF) is one of the most effective methods to drive liquids into the channels. EOF mobility is affected by a property of the micromachined glass surfaces, which includes surface roughness that is determined by the manufacturing processes. In this paper, we investigate the effect of micromaching processes on the glass surface topography and the EOF mobility. We prepared glass surfaces by either wet etching or by NLD plasma etching, investigated the surface topography using atomic force microscopy, and attempted to correlate it with EOF generated in the micro-channels of the machined glass. Experiments revealed that the EOF mobility strongly depends on the surface roughness, and therefore upon the fabrication process used. A particularly strong dependency was observed when the surface roughness was on the order of the electric double layer thickness or below. We believe that the correlation described in this paper can be of great help in the design of micro/nano fluidic devices.

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Section: Introductionmentioning

confidence: 99%

Effects of Micromachining Processes on Electro-Osmotic Flow Mobility of Glass Surfaces

et al. 2013

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“…However, mainly flat surfaces are structured so far, arisen from the usually used production methods, mostly developed for the semiconductor industry [3,[6][7][8]. Additionally, one of the major problems of mass production methods like injection molding is, that free formed surfaces yield undercuts in the demolding direction, leading to a high risk of deforming or in worst case ripping during the demolding process [9].…”

Section: Introductionmentioning

confidence: 99%

Influence of the process parameters on the replication of microstructured freeform surfaces

Burgsteiner

Müller

Lucyshyn

et al. 2014

AIP Conference Proceedings

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-Surfaces of technical parts are getting more and more attention in terms of functionalization. By modification, additional functionality is given to the part, e.g. self-cleaning effect or antireflection behavior. Nowadays mainly flat surfaces are structured which is a consequence of the available production methods. However, the demand of micro structured free form surfaces is increasing, enabling novel products. A major problem in the mass production (e.g. injection molding) of structured freeform surfaces is to demold these structures without ripping or deforming them due to occurring undercuts. Recently a novel concept was developed which overcomes this limitation. A nickel substrate containing a structure composed of lines orientated in two different directions, one orientated in melt flow direction, the other one perpendicular to that, but both with a cross-section of approximately 45 μm x 55 μm (w x h) was used as a premaster to cast a flexible master. This master made of poly(dimethylsiloxane) (PDMS) was mounted on a bending edge in an injection mold cavity. Within this paper the influence of process parameters on the replication grade of the structure lines depending on the structure orientation was evaluated, varying the holding pressure, melt and mold temperature using statistical design of experiment methods. The replication grade was evaluated by characterizing the shape of the structure lines along the entire process chain, using an infinite focus system. The results show, that the melt temperature has the biggest influence on the dimensions of the structures, the mold temperature only a slight one.

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“…These devices utilize microfluidic channels to control fluid flow to regions of the chip where a variety of procedures can take place including reagent mixing, affinity based binding, signal transduction, and cell culturing [1][2][3][4] . Microfluidics provides many advantages over conventional clinical diagnostic tools such as microwell plate readers or electrophoretic gel shift assays.…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Ben‐Yoav

Dykstra

Gordonov

et al. 2014

JoVE

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Miniaturization of analytical benchtop procedures into the micro-scale provides significant advantages in regards to reaction time, cost, and integration of pre-processing steps. Utilizing these devices towards the analysis of DNA hybridization events is important because it offers a technology for real time assessment of biomarkers at the point-of-care for various diseases. However, when the device footprint decreases the dominance of various physical phenomena increases. These phenomena influence the fabrication precision and operation reliability of the device. Therefore, there is a great need to accurately fabricate and operate these devices in a reproducible manner in order to improve the overall performance. Here, we describe the protocols and the methods used for the fabrication and the operation of a microfluidic-based electrochemical biochip for accurate analysis of DNA hybridization events. The biochip is composed of two parts: a microfluidic chip with three parallel micro-channels made of polydimethylsiloxane (PDMS), and a 3 x 3 arrayed electrochemical micro-chip. The DNA hybridization events are detected using electrochemical impedance spectroscopy (EIS) analysis. The EIS analysis enables monitoring variations of the properties of the electrochemical system that are dominant at these length scales. With the ability to monitor changes of both charge transfer and diffusional resistance with the biosensor, we demonstrate the selectivity to complementary ssDNA targets, a calculated detection limit of 3.8 nM, and a 13% cross-reactivity with other non-complementary ssDNA following 20 min of incubation. This methodology can improve the performance of miniaturized devices by elucidating on the behavior of diffusion at the micro-scale regime and by enabling the study of DNA hybridization events. Video LinkThe video component of this article can be found at

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Polymeric microfluidic system for DNA analysis

Cited by 103 publications

References 160 publications

Effects of Micromachining Processes on Electro-Osmotic Flow Mobility of Glass Surfaces

Effects of Micromachining Processes on Electro-Osmotic Flow Mobility of Glass Surfaces

Influence of the process parameters on the replication of microstructured freeform surfaces

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

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