Polymeric film of 6-arm-poly(ethylene glycol) amine graphene oxide with poly (ε-caprolactone): Adherence and growth of adipose derived mesenchymal stromal cells culture on rat bladder

Durán, Nélson; Luzo, Angela C.; Duarte, Adriana Silva Santos; Volpe, Bruno Bosch; Ceragioli, Helder José; Andrade, Patrícia Fernanda; Souza, J. C.; Fávaro, Wagner José

doi:10.1088/1742-6596/838/1/012035

Cited by 4 publications

(7 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, GO-COOH was able to promote an increase in the toxicity of DOX compared to the drug in the free form and could play a satisfactory effect considering the in vivo application for the treatment of NMIBC. 10 Econanotoxicology of GO exhibited nontoxic effect in the in vitro or in vivo assay with the standard organisms, excepting with Hydra for 96 h of contact. 8 …”

Section: Cell Viabilitymentioning

confidence: 97%

“…9 Briefly: a sample of 10 mg of GO (carboxylated) 10 was dispersed in deionized water and 10 mg of previously dispersed six-armed PEG (PEG (6 ARM-poly(ethylene glycol) amine or 6 ARM-PEG-amine) (15 kD) in the deionized water was added to the GO dispersion and kept in an ultrasonic bath for 5 min. Ten mg of hydrochloride 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) dissolved in deionized water was added to the mixture, with stirring for 15 min.…”

Section: Graphene Oxide As Scaffoldmentioning

confidence: 99%

“…11 The oxidized GO and doxorubicin was prepared as also an intermediate following the procedure previously reported. 10 Attenuated total reflectance (ATR), X-ray diffraction analysis (XRD) and X-ray excited photoelectron spectroscopy (XPS) were used for characterization, besides other usual techniques. 10 The size and zeta potential of this nanoparticles were 149.0 ± 10.0 nm and +37.2 ± 0.6 mV, respectively.…”

Section: Graphene Oxide As Scaffoldmentioning

confidence: 99%

“…10 Attenuated total reflectance (ATR), X-ray diffraction analysis (XRD) and X-ray excited photoelectron spectroscopy (XPS) were used for characterization, besides other usual techniques. 10 The size and zeta potential of this nanoparticles were 149.0 ± 10.0 nm and +37.2 ± 0.6 mV, respectively. All the intermediates were also analyzed and described on Table 1.…”

Section: Graphene Oxide As Scaffoldmentioning

confidence: 99%

“…The intermediates in the synthesis of GO-PEG-PEI are shown in the Table 1 and the final product was 153 ± 10 nm and zeta potential of -36.2 ± 0.9 mV ( Figure 5). 10 …”

Section: Graphene Oxide As Scaffoldmentioning

confidence: 99%

See 4 more Smart Citations

Nanopharmaceuticals and Their Applications in Bladder Cancer Therapy: a Mini Review

Durán

Fávaro

2018

J. Braz. Chem. Soc.

Self Cite

View full text Add to dashboard Cite

The primary treatment for high-grade non-muscle invasive bladder cancer (NMIBC) is based on surgery by transurethral resection of bladder tumor (TURBT), followed by intravesical immunotherapy with Bacillus Calmette-Guerin (BCG) to prevent recurrence and to reduce the tumor progression. However, BCG therapy shows several undesirable effects. The current treatment on NMIBC is doxorubicin (DOX), but with high toxicity. Our nanotechnology strategy was done through scaffolds for the NMIBC treatment: graphene oxide (GO) and a nanostructured lipid carrier (NLC). A GO hybrid for administration of DOX and small interfering RNA (siRNA) was developed. This hybrids administered in vivo against NMIBC in rats gave absence of lesions. NLC was prepared by using a mixture of two lipids stabilized by a surfactant and DOX by high homogenization pressure technique. In this case showed a 20% of the animals exhibited benign lesions (papillary hyperplasia), however, in the presence of siRNA reached 40% of rats with benignant lesions. These two scaffolds are potential new drugs for DOX for bladder cancer treatment without any cardiotoxicity problems.

show abstract

Section: Cell Viabilitymentioning

confidence: 97%

Section: Graphene Oxide As Scaffoldmentioning

confidence: 99%

Section: Graphene Oxide As Scaffoldmentioning

confidence: 99%

Section: Graphene Oxide As Scaffoldmentioning

confidence: 99%

“…The intermediates in the synthesis of GO-PEG-PEI are shown in the Table 1 and the final product was 153 ± 10 nm and zeta potential of -36.2 ± 0.9 mV ( Figure 5). 10 …”

Section: Graphene Oxide As Scaffoldmentioning

confidence: 99%

See 3 more Smart Citations

Nanopharmaceuticals and Their Applications in Bladder Cancer Therapy: a Mini Review

Durán

Fávaro

2018

J. Braz. Chem. Soc.

Self Cite

View full text Add to dashboard Cite

show abstract

Short review on the use of graphene as a biomaterial –prospects, and challenges in Brazil

Souza

Cambraia²,

Gomide³

et al. 2022

Journal of Materials Research and Technology

View full text Add to dashboard Cite

Hybrid graphene oxide as carrier of doxorubicin: cytotoxicity and preliminary in vivo assays against bladder cancer

Fávaro

Souza

Ferreira

et al. 2020

Adv. Nat. Sci: Nanosci. Nanotechnol.

Self Cite

View full text Add to dashboard Cite

Two types of graphene oxide (GO) hybrids were synthesised for the administration of doxorubicin (DOX, named GO-CO-DOX) and interfering RNA - siRNA (named GO-PEG-PEI/siRNA). These nanomaterials were used for intervention on vascular endothelial growth factor (VEGF) that represents an important strategy to block the formation of new vessels to inhibit cancer progression. For the delivery of DOX, it was incorporated into GO, while for the delivery of siRNA, GO was covalently bonded with the cationic polyethyleneimine (PEI) and then complexed with siRNA. The nanostructures were characterised by attenuated total reflection ATR-FTIR, zeta potential, x-ray photoelectron spectroscopy (XPS), x-ray diffraction (XRD), and transmission electron microscopy (TEM). The cytotoxicity studies with GO-PEG-PEI and GO-PEG-PEI/siRNA systems showed that both formulations have IC50 values of around 100 μg ml−1. The systems were administered in vivo to investigate their antitumor effects against non-muscle-invasive bladder cancer (NMIBC) and showed to be promising for the treatment of NMIBC.

show abstract

Polymeric film of 6-arm-poly(ethylene glycol) amine graphene oxide with poly (ε-caprolactone): Adherence and growth of adipose derived mesenchymal stromal cells culture on rat bladder

Cited by 4 publications

References 15 publications

Nanopharmaceuticals and Their Applications in Bladder Cancer Therapy: a Mini Review

Nanopharmaceuticals and Their Applications in Bladder Cancer Therapy: a Mini Review

Short review on the use of graphene as a biomaterial –prospects, and challenges in Brazil

Hybrid graphene oxide as carrier of doxorubicin: cytotoxicity and preliminary in vivo assays against bladder cancer

Contact Info

Product

Resources

About