Polymer-coated magnetic nanoparticles for the efficient capture of Mycobacterium tuberculosis (Mtb)

Smit, Marica; Lutz, Marietjie

doi:10.1007/s42452-020-03403-9

Cited by 6 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although both magnetite and maghemite are the most studied due to their electrical and magnetic properties, it is the magnetite that has the strongest magnetic properties (He et al 2015, Smit 2018. Therefore, magnetite has been used in more biomedical applications.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis, characterization and bio-functionalization of magnetic nanoparticles to improve the diagnosis of tuberculosis

et al. 2020

View full text Add to dashboard Cite

Mycobacterium tuberculosis is the cause of one of the diseases with the highest mortality and morbidity rate in the Americas and in the world. In developing countries, the diagnosis of tuberculosis (TB) is based on baciloscopy and bacteriological cultures. The first method has a low sensitivity, and the second can take several weeks to reach a confirmatory diagnosis. The lack of a rapid diagnosis compromises the efforts to control this disease and favors the transmission of tuberculosis to the susceptible population. In this work, we present the synthesis, amine-silanization, characterization and bio-functionalization of magnetic nanoparticles (MNPs) to develop a sandwich ELISA to detect and concentrate antigens from M. tuberculosis. For this purpose, a recombinant mycobacterial heat shock protein Hsp16.3, which contributes to the persistence of TB, was cloned and expressed in the E. coli system. Polyclonal antibodies anti-Hsp16.3 were produced in a rabbit and in mice. Magnetic nanoparticles were synthesized by co-precipitation, amine-functionalized and characterized by several physical-chemical methods. The XRD, Mossbauer spectroscopy, zeta potential, TEM, and FTIR all proved the successful preparation of the MNPs showing a diffraction crystal diameter of 10.48 ± 2.56 nm, superficial net charge of : +23.57 ± 2.87 mV, characteristic patterns of magnetite and a structure similar to a sphere. Additionally, it showed a magnetization saturation of 37.06 emu.g−1. For the functionalization of nanoparticle surfaces with anti-Hsp16.3, the active ester method was used for bond formation, and parameters such as time of incubation, coupling agents ratio (EDC/NHS) and concentration as well as surface saturation level of amine-silanized MNPs (MNP@Si@NH2) were standardized. Finally, bio-functionalized MNPs were used to detect, fix and concentrate the recombinant antigen Hsp16.3 from M. tuberculosis in a sandwich ELISA-MNP assay.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Nanoparticles have been widely studied because they have unique properties compared to bulk. These properties are determined by the size, structure, and physical-chemical composition of the surface and its core (Smit 2018).…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization and bio-functionalization of magnetic nanoparticles to improve the diagnosis of tuberculosis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Nevertheless, due to low stability in an acidic aqueous solution, it is necessary to modify MM to produce a more stable magnetic composite material [26]. The stabilization of MM can be increased by chitosan coating [27], but using an inappropriate amount of chitosan can reduce the magnetic properties of MM [28]. Adding chitosan to MM, which is not proportional, produced an irregular structural morphology [29].…”

Section: ■ Introductionmentioning

confidence: 99%

Preparation and Characterization of Magnetic Material/Chitosan Composite Modified with Glycidyl-Trimethylammonium Chloride

Mukhayani,

Kunarti,

Kamiya

et al. 2024

Indones. J. Chem.

View full text Add to dashboard Cite

Glycidyl-trimethylammonium chloride (GTMAC) containing quaternary ammonium (QA) groups is commonly used as a base catalyst for any organic reaction. This research prepared a novel composite of GTMAC attached to chitosan-coated magnetic material (MM/Chi/GTMAC) using a precipitation method. The effect of chitosan and GTMAC contents on MM/chi/GTMAC properties was studied, where the chitosan content varied from 0, 0.3, 0.5, 1.0, and 3.0 mol, and GTMAC varied from 0, 0.3, 0.8, 1.0, 1.5, and 3 mL with the constant mass of MM (0.4640 g). The physicochemical and morphological properties were characterized with FTIR, SEM-EDX, XRD, TGA, UV-vis, AAS, and zeta-sizer, and the magnetic strength was simply tested with an external magnet. The result showed that a mixture containing chitosan and GTMAC of 0.358 g and 1.5 mL was an optimum composition, in which MM/chi(0.5)/GTMAC(1.5) has high thermal stability, low chitosan and Fe solubility, and optimum content of QA (0.284 mol/g) without loss of magnetic strength. The higher the amount of chitosan, the lower the magnetic properties, and the higher the GTMAC did not increase the QA content. Therefore, the composite produced has the potential to be a novel heterogeneous base catalyst that is quickly recovered from any organic reaction media.

show abstract

Clinically Relevant Metallic Nanoparticles in Tuberculosis Diagnosis and Therapy

Akinnawo,

Dube

2024

Advanced Therapeutics

View full text Add to dashboard Cite

Globally a significant burden of tuberculosis (TB) is faced, which is difficult to eradicate due to patients' non‐adherence, and drug‐resistant strains that are spreading at an alarming rate. Novel approaches are required to improve diagnosis and treatment. Metallic nanoparticles (MNPs) have demonstrated potential as sensor probes and in combination therapy, which combines MNPs with antimycobacterial drugs to develop new treatment and theranostic approaches. To strengthen the theoretical foundation toward the clinical application of TB nanomedicine, this review focuses on the properties and effectiveness of therapeutically relevant MNPs. It also elaborates on their antimycobacterial mechanisms. This review aims to analyze the body of literature on the topic, pinpoint important empirical findings, and identify knowledge gaps that can provide a basis for future research endeavors and translation of the technologies. Current data suggest that MNPs are potential systems for efficient diagnosis and treatment although additional pre‐clinical and clinical research is needed to bring these technologies to the clinic.

show abstract

Polymer-coated magnetic nanoparticles for the efficient capture of Mycobacterium tuberculosis (Mtb)

Cited by 6 publications

References 24 publications

Synthesis, characterization and bio-functionalization of magnetic nanoparticles to improve the diagnosis of tuberculosis

Synthesis, characterization and bio-functionalization of magnetic nanoparticles to improve the diagnosis of tuberculosis

Preparation and Characterization of Magnetic Material/Chitosan Composite Modified with Glycidyl-Trimethylammonium Chloride

Clinically Relevant Metallic Nanoparticles in Tuberculosis Diagnosis and Therapy

Contact Info

Product

Resources

About