1999
DOI: 10.1002/(sici)1522-9505(19990701)268:1<69::aid-apmc69>3.3.co;2-4 View full text |Buy / Rent full text
|
|

Abstract: SUMMARY: Samples of glucans and mannans isolated from yeast, Saccharomyces cerevisiae, were characterized using polymer analytical methods:13 C and 1 H NMR spectroscopy, IR spectroscopy and light scattering coupled with size exclusion chromatography (SEC/MALLS). Despite partially incomplete reactions, high-molar-mass mannans with a trimodal distribution were obtained with all the enzyme systems. Mannoprotein with a molar mass of 410 000 g mol -1 was obtained using a recombinant glucanase that was completely vo… Show more

Help me understand this report

Search citation statements

Order By: Relevance
Select...
1
1
1
1
2
2
0

Year Published

2009
2009
2022
2022

Publication Types

Select...
3

Relationship

0
3

Authors

Journals

2
2
0
Order By: Relevance
“…A strong band between 975 and 1130 cm −1 was assigned to the stretching vibration of pyranose ring. The sharp peak at 814 cm −1 was attributed to the characteristic absorption of α-glycosidic bond of mannan [ 30 , 31 ], which was consistent with the results of monosaccharide composition.…”
Section: Resultssupporting
Create an account to read the remaining citation statements from this report. You will also get access to:
  • Search over 1b+ citation statments to see what is being said about any topic in the research literature
  • Advanced Search to find publications that support or contrast your research
  • Citation reports and visualizations to easily see what publications are saying about each other
  • Browser extension to see Smart Citations wherever you read research
  • Dashboards to evaluate and keep track of groups of publications
  • Alerts to stay on top of citations as they happen
  • Automated reference checks to make sure you are citing reliable research in your manuscripts
  • 14 day free preview of our premium features.

Trusted by researchers and organizations around the world

Over 100,000 students researchers, and industry experts at use scite

See what students are saying

rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…A strong band between 975 and 1130 cm −1 was assigned to the stretching vibration of pyranose ring. The sharp peak at 814 cm −1 was attributed to the characteristic absorption of α-glycosidic bond of mannan [ 30 , 31 ], which was consistent with the results of monosaccharide composition.…”
Section: Resultssupporting
“…Whereas mannoproteins purified from white wines (molecular weight also determined by MALLS) ranged from 30 to 300 kDa (Gonçalves et al, 2002). When testing different methods for mannoprotein extraction from brewery and bakery S. cerevisiae strains, Kulicke & Kath observed that the highest molecular weights (Mw of 410 kDa determined through HPSEC-MALS) were obtained when mannoproteins were extracted with commercial β-glucanases completely free of protease activity (Kath & Kulicke, 1999), as in the present study. where α, νG, and νH are the corresponding hydrodynamic coefficients and Kα, Kg, and Kh the corresponding constants.…”
Section: Molar Mass Distribution and Molecular Propertiessupporting
“…In more controlled systems, enzymatic extractions are usually performed with commercial enzymes. Nevertheless, besides βglucanases, other residual hydrolytic activities have been observed in these commercial cocktails, especially proteases and mannanases that can directly affect the mannoprotein structure (De Iseppi et al, 2019;Kath & Kulicke, 1999;Schiavone et al, 2014). As expected, mannoproteins extracted by physicochemical methods (heat, acid, and alkali extractions) also differ in molar mass range and composition from those extracted through enzymes (De Iseppi et al, 2019;Kath & Kulicke, 1999;Moine-Ledoux & Dubourdieu, 1999;Núñez et al, 2006;Saunier, Mercereaua, & Vezinhet, 1991).…”
Section: Introductionmentioning
“…Mannan of Wt cells (CAF2) and CHK21 were prepared for monosaccharide composition analysis by the alditol acetate method (Dean, 1995; Ciucanu and Kerek, 1984; Kath and Kulicke, 1999; Sawardeker et al, 1967). The glycosyl hydrolyses were carried out with 4M-trifluoroacetic acid at 105°C for 5 h followed by reduction in H 2 O with NaBD 4 overnight at room temperature, and subsequent acetylation by acetic anhydride at 100°C for 2 h. The alditol acetate derivatives were analyzed by gas liquid chromatography (GLC) using a Varian 3400 gas chromatograph equipped with a 30-m DB-17 capillary column [210°C (30 min)→240°C at 2°C/min], and by GLC-mass spectrometry (GLC-MS) in the electron-impact and chemical-ionization modes in a ThermoFinigan PolarisQ instrument.…”
Section: Methodsmentioning