Polyhydroxybutyrate (PHB) production by Cupriavidus necator from sugarcane vinasse and molasses as mixed substrate

Dalsasso, Raul Remor; Pavan, Felipe André; Bordignon, Sidnei Emílio; Aragão, Gláucia Maria Falcão de; Poletto, Patrícia

doi:10.1016/j.procbio.2019.07.007

Cited by 104 publications

(58 citation statements)

References 35 publications

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“…This study used the environmentally-friendly pretreatment method for preparing pineapple peel waste. Many previous publications abundantly used multiple supplements and chemical pretreatment substrates [29,60,[65][66][67]. To the best of the author's knowledge, there is only one report on PHAs production by microorganisms using chemical pretreated pineapple peel as substrates [60].…”

Section: Plos Onementioning

confidence: 99%

“…In addition to the type of microorganism used, the use of inexpensive substrates has become increasingly important because the substrate or carbon source is the major cost factor in PHAs production [28]. For instance, Dalsasso et al used vinasse and sugarcane molasses as substrate for PHB production [29,30]. Additionally, a new Methylobacterium isolate produced 0.55 g/L PHB using methanol as a sole carbon source under two-stage fermentation [31].…”

Section: Introductionmentioning

confidence: 99%

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Response surface method for polyhydroxybutyrate (PHB) bioplastic accumulation in Bacillus drentensis BP17 using pineapple peel

et al. 2020

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Polyhydroxybutyrate (PHB) is a biodegradable biopolymer which is useful for various applications including packing, medical and coating materials. An endospore-forming bacterium (strain BP17) was isolated from composted soil and evaluated for PHB production. Strain BP17, taxonomically identified as Bacillus drentensis, showed enhanced PHB accumulation and was selected for further studies. To achieve maximum PHB production, the culture conditions for B. drentensis BP17 were optimized through response surface methodology (RSM) employing central composite rotatable design (CCRD). The final optimum fermentation conditions included: pineapple peel solution, 11.5% (v/v); tryptic soy broth (TSB), 60 g/ L; pH, 6.0; inoculum size, 10% (v/v) and temperature, 28˚C for 36 h. This optimization yielded 5.55 g/L of PHB compared to the non-optimized condition (0.17 g/L). PHB accumulated by B. drentensis BP17 had a polydispersity value of 1.59 and an average molecular weight of 1.15x10 5 Da. Thermal analyses revealed that PHB existed as a thermally stable semi-crystalline polymer, exhibiting a thermal degradation temperature of 228˚C, a melting temperature of 172˚C and an apparent melting enthalpy of fusion of 83.69 J/g. It is evident that B. drentensis strain BP17 is a promising bacterium candidate for PHB production using agricultural waste, such as pineapple peel as a low-cost alternative carbon source for PHB production.

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Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Response surface method for polyhydroxybutyrate (PHB) bioplastic accumulation in Bacillus drentensis BP17 using pineapple peel

et al. 2020

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“…The main natural sources for obtaining PHA, especially different types of PHB, are heterotrophic bacteria, achieving good yields, with accumulation of PHB up to 85% dry cell weight (dcw) in Cupriavidus necator [ 39 ]. In addition to the good yields obtained, these species proved to be competent in assimilating alternative carbon sources for the production of PHA, using vinasse and molasses from sugar production and even waste frying oil [ 40 , 41 ]. Due to the relative ease in using genetic engineering with these bacteria, several studies deal with the production by recombinant organisms or even heterologous expression in Escherichia coli , with good results, such as the yield of 70–90% (dcw) [ 42 , 43 , 44 ].…”

Section: Bioplasticsmentioning

confidence: 99%

“…One way to mitigate these high costs in cultivation is using renewable and cheap carbon sources, such as domestic and industrial waste, which applies to heterotrophic bacteria [ 40 , 41 , 48 ] as well as photosynthetic organisms, that require fewer nutrients for their growth and production of biomass and biotechnological metabolites, such as bioplastic [ 51 , 52 ], thus, producing biopolymers basically from light and CO 2 [ 14 ]; in this context, we can understand the potential of the so-called blue algae, the cyanobacteria.…”

Section: Bioplasticsmentioning

confidence: 99%

Cyanobacterial Polyhydroxyalkanoates: A Sustainable Alternative in Circular Economy

2020

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Conventional petrochemical plastics have become a serious environmental problem. Its unbridled use, especially in non-durable goods, has generated an accumulation of waste that is difficult to measure, threatening aquatic and terrestrial ecosystems. The replacement of these plastics with cleaner alternatives, such as polyhydroxyalkanoates (PHA), can only be achieved by cost reductions in the production of microbial bioplastics, in order to compete with the very low costs of fossil fuel plastics. The biggest costs are carbon sources and nutrients, which can be appeased with the use of photosynthetic organisms, such as cyanobacteria, that have a minimum requirement for nutrients, and also using agro-industrial waste, such as the livestock industry, which in turn benefits from the by-products of PHA biotechnological production, for example pigments and nutrients. Circular economy can help solve the current problems in the search for a sustainable production of bioplastic: reducing production costs, reusing waste, mitigating CO2, promoting bioremediation and making better use of cyanobacteria metabolites in different industries.

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“…A method to obtain environmentally friendly or compatible with a living organism polyurethane can be to use a natural compound for its synthesis, e.g., polyhydroxybutyrate (PHB). PHB is the most popular compound among all polyhydroxyalkanoates due its biocompatibility, biodegradability and natural origin [1][2][3]. However, despite the undoubted benefits of using PHB, its high crystallinity, brittleness, high production costs, poor mechanical properties and thermal instability cause that its use is limited.…”

Section: Introductionmentioning

confidence: 99%

Predicted Studies of Branched and Cross-Linked Polyurethanes Based on Polyhydroxybutyrate with Polycaprolactone Triol in Soft Segments

et al. 2020

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The number of cross-links in the non-linear polyurethane structure is the basic factor affecting its properties. Selected properties of aliphatic polyurethanes with soft segments made of different amounts of polycaprolactonetriol, polycaprolactonediol and synthetic, telechelic poly([R,S]-3-hydroxybutyrate) were determined. On the basis of changes in polyurethane properties, the correlation between these properties and the construction of soft segments was found. The structure of polyurethanes, their morphology, hydrophilicity, thermal and mechanical properties were examined. These properties were changed linearly up to 15% content of polycaprolactonetriol in soft segments. A further increase in the amount of triol causes that these properties are mainly determined by the high number of cross-links.

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Polyhydroxybutyrate (PHB) production by Cupriavidus necator from sugarcane vinasse and molasses as mixed substrate

Cited by 104 publications

References 35 publications

Response surface method for polyhydroxybutyrate (PHB) bioplastic accumulation in Bacillus drentensis BP17 using pineapple peel

Response surface method for polyhydroxybutyrate (PHB) bioplastic accumulation in Bacillus drentensis BP17 using pineapple peel

Cyanobacterial Polyhydroxyalkanoates: A Sustainable Alternative in Circular Economy

Predicted Studies of Branched and Cross-Linked Polyurethanes Based on Polyhydroxybutyrate with Polycaprolactone Triol in Soft Segments

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