Polyhydroxyalkanoate (PHA) synthase genes and PHA-associated gene clusters in Pseudomonas spp. and Janthinobacterium spp. isolated from Antarctica

Tan, İlhan; Foong, Choon Pin; Tan, Hua; Lim, Hui; Zain, Noor-Afiqah Ahmad; Tan, Yung-Chie; Hoh, Chee Choong; Sudesh, Kumar

doi:10.1016/j.jbiotec.2020.03.006

Cited by 35 publications

(15 citation statements)

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“…Genomes from Janthinobacterium sp. isolates recovered from Antarctica were found to contain hitherto uncharacterized putative PHA synthase genes that were phylogenetically divergent from the known PhaC classes and tentatively labeled as “Class V” PhaCs ( Tan et al, 2020 ). The first occurrence of PHA producers from hypersaline microbial mats were discovered in salt concentration ponds located in Mexico through PCR amplification of phaC genes ( Martínez-Gutiérrez et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Developing Bioprospecting Strategies for Bioplastics Through the Large-Scale Mining of Microbial Genomes

et al. 2021

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The accumulation of petroleum-based plastic waste has become a major issue for the environment. A sustainable and biodegradable solution can be found in Polyhydroxyalkanoates (PHAs), a microbially produced biopolymer. An analysis of the global phylogenetic and ecological distribution of potential PHA producing bacteria and archaea was carried out by mining a global genome repository for PHA synthase (PhaC), a key enzyme involved in PHA biosynthesis. Bacteria from the phylum Actinobacteria were found to contain the PhaC Class II genotype which produces medium-chain length PHAs, a physiology until now only found within a few Pseudomonas species. Further, several PhaC genotypes were discovered within Thaumarchaeota, an archaeal phylum with poly-extremophiles and the ability to efficiently use CO2 as a carbon source, a significant ecological group which have thus far been little studied for PHA production. Bacterial and archaeal PhaC genotypes were also observed in high salinity and alkalinity conditions, as well as high-temperature geothermal ecosystems. These genome mining efforts uncovered previously unknown candidate taxa for biopolymer production, as well as microbes from environmental niches with properties that could potentially improve PHA production. This in silico study provides valuable insights into unique PHA producing candidates, supporting future bioprospecting efforts toward better targeted and relevant taxa to further enhance the diversity of exploitable PHA production systems.

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Section: Discussionmentioning

confidence: 99%

Developing Bioprospecting Strategies for Bioplastics Through the Large-Scale Mining of Microbial Genomes

et al. 2021

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“…This could be explained either by non-specific binding of the degenerate primers or the existence of other copies of the gene in close proximity to each other, simultaneous carrying of two types of synthases or other unusual PHA synthase related genes. Recent studies [ 28 ] have shown, that Pseudomonas spp. isolated from Antarctica carried two classes of synthases (I and II) and some Janthinobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

Biodiversity and Habitats of Polar Region Polyhydroxyalkanoic Acid-Producing Bacteria: Bioprospection by Popular Screening Methods

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et al. 2020

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Polyhydroxyalkanoates (PHAs), the intracellular polymers produced by various microorganisms as carbon and energy storage, are of great technological potential as biodegradable versions of common plastics. PHA-producing microbes are therefore in great demand and a plethora of different environments, especially extreme habitats, have been probed for the presence of PHA-accumulators. However, the polar region has been neglected in this regard, probably due to the low accessibility of the sampling material and unusual cultivation regime. Here, we present the results of a screening procedure involving 200 bacterial strains isolated from 25 habitats of both polar regions. Agar-based tests, microscopy, and genetic methods were conducted to elucidate the biodiversity and potential of polar-region PHA-accumulators. Microscopic observation of Nile Red stained cells proved to be the most reliable screening method as it allowed to confirm the characteristic bright orange glow of the Nile Red–PHA complex as well as the typical morphology of the PHA inclusions. Psychrophilic PHA-producers belonged mostly to the Comamonadaceae family (Betaproteobacteria) although actinobacterial PHA synthesizers of the families, Microbacteriaceae and Micrococcaceae also featured prominently. Glacial and postglacial habitats as well as developed polar region soils, were evaluated as promising for PHA-producer bioprospection. This study highlights the importance of psychrophiles as biodiverse and potent polyhydroxyalkanoate sources for scientific and application-aimed research.

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“…An important predisposition of PHA production by prokaryotes is the presence of genes encoding a crucial enzyme required for PHA synthesis-PHA synthase (PhaC). These enzymes are divided into four classes based on substrate specificity and subunit composition [43]. The thermophilic bacterium Tepidimonas taiwanensis LMG 22826 can be a suitable candidate for PHA production [37].…”

Section: Pha-related Genes Of Strain T Taiwanensis Lmg 22826mentioning

confidence: 99%

Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis

et al. 2021

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Polyhydroxyalkanoates (PHA) are microbial polyesters that have recently come to the forefront of interest due to their biodegradability and production from renewable sources. A potential increase in competitiveness of PHA production process comes with a combination of the use of thermophilic bacteria with the mutual use of waste substrates. In this work, the thermophilic bacterium Tepidimonas taiwanensis LMG 22826 was identified as a promising PHA producer. The ability to produce PHA in T. taiwanensis was studied both on genotype and phenotype levels. The gene encoding the Class I PHA synthase, a crucial enzyme in PHA synthesis, was detected both by genome database search and by PCR. The microbial culture of T. taiwanensis was capable of efficient utilization of glucose and fructose. When cultivated on glucose as the only carbon source at 50°C, the PHA titers reached up to 3.55 g/L, and PHA content in cell dry mass was 65%. The preference of fructose and glucose opens the possibility to employ T. taiwanensis for PHA production on various food wastes rich in these abundant sugars. In this work, PHA production on grape pomace extracts was successfully tested.

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Polyhydroxyalkanoate (PHA) synthase genes and PHA-associated gene clusters in Pseudomonas spp. and Janthinobacterium spp. isolated from Antarctica

Cited by 35 publications

References 56 publications

Developing Bioprospecting Strategies for Bioplastics Through the Large-Scale Mining of Microbial Genomes

Developing Bioprospecting Strategies for Bioplastics Through the Large-Scale Mining of Microbial Genomes

Biodiversity and Habitats of Polar Region Polyhydroxyalkanoic Acid-Producing Bacteria: Bioprospection by Popular Screening Methods

Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis

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