Polyethylenimine-functionalized cationic barley β-glucan derivatives for macrophage RAW264.7 cell-targeted gene delivery systems

Lee, Hye Ji; Park, Jaehong; Lee, Gyeong Jin; Oh, Jae‐Min; Kim, Tae-Il

doi:10.1016/j.carbpol.2019.115324

Cited by 5 publications

(5 citation statements)

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“…The evidences suggest that the derivatives are able to recognize and bind to membrane receptors of such particular cell type. [173] The mannosylation of PEI chains was demonstrated to be useful for targeting specific cell types for self-amplifying RNA (saRNA) delivery and expression. [174] Overall, sugar decoration of PEI is able to enhance the cellular uptake of polyplexes due to the suitable affinity of the moieties to components present in the cell membranes, and the approach typically enhances serum stability and biocompatibility (increased cell viability is normally monitored).…”

Section: Sugar Decoration Of Pei Chainsmentioning

confidence: 99%

Current Designs of Polymeric Platforms Towards the Delivery of Nucleic Acids Inside the Cells with Focus on Polyethylenimine

Oliveira

Albuquerque

Delecourt

et al. 2021

CGT

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Background: Gene delivery is a promising technology for treating diseases linked to abnormal gene expression. Since nucleic acids are the therapeutic entities in such approach, a transfecting vector is required because the macromolecules are not able to efficiently enter the cells by themselves. Viral vectors have been evidenced to be highly effective in this context; however, they suffer from fundamental drawbacks, such as the ability to stimulate immune responses. The development of synthetic vectors has accordingly emerged as an alternative. Objectives: Gene delivery by using non-viral vectors is a multi-step process that poses many challenges, either regarding the extracellular or intracellular media. We explore the delivery pathway and afterwards, we review the main classes of non-viral gene delivery vectors. We further focus on the progresses concerning polyethylenimine-based polymer-nucleic acid polyplexes, which have emerged as one of the most efficient systems for delivering genetic material inside the cells. Discussion: The complexity of the whole transfection pathway, along with a lack of fundamental understanding, particularly regarding the intracellular trafficking of nucleic acids complexed to non-viral vectors, probably justifies the current (beginning of 2021) limited number of formulations that have progressed to clinical trials. Truly, successful medical developments still require a lot of basic research. Conclusion: Advances in macromolecular chemistry and high-resolution imaging techniques will be useful to understand fundamental aspects towards further optimizations and future applications. More investigations concerning the dynamics, thermodynamics and structural parameters of polyplexes would be valuable since they can be connected to the different levels of transfection efficiency hitherto evidenced.

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Section: Sugar Decoration Of Pei Chainsmentioning

confidence: 99%

Current Designs of Polymeric Platforms Towards the Delivery of Nucleic Acids Inside the Cells with Focus on Polyethylenimine

Oliveira

Albuquerque

Delecourt

et al. 2021

CGT

View full text Add to dashboard Cite

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“…Nanomaterials are intensively studied as the carriers for gene delivery in animal cells [12][13][14][15]. However, their potential for plants remains understudied [1,5].…”

Section: Introductionmentioning

confidence: 99%

Transformation of the moss (Ceratodon purpureus) with plasmid DNA delivered by novel block-copolymers of the dimethylaminoethyl methacrylate

et al. 2022

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To investigate the potential of poly(2-dimethylamino)ethyl methacrylate (DMAEMA)based block-like polymers to serve as gene delivery carriers in moss Ceratodon purpureus (Hedw.) Brid. protoplasts, and to evaluate the level of their phytotoxicity. Methods. Organic synthesis; DNA gel retardation assay; adapted PEG-mediated transformation protocol; PCR; light microscopy. Results. The formation of pDNA complex with DMAEMA-based carriers took place at 0.01-0.1 % concentrations of the polymer. The poly-DMAEMA carriers F8-DM1, F8-DM2 (fluorine-containing), LAcr-DM1, LAcr-DM2 (lauryl acrylate-containing), BAcr-DM1, and BAcr-DM2 (butyl acrylate-containing) were effective as carriers of plasmid DNA pSF3 at C. purpureus transformation. PCR analysis confirmed that the transformants of C. purpureus moss contain GFP as a gene of interest after the protoplast transformation by polymers LAcr-DM2, LAcr-DM1, BAcr-DM2, BAcr-DM1 and F8-DM2. The poly-DMAEMA carriers at working concentration (0.0025 %) were relatively non-toxic for protoplasts of C. purpureus moss. 83.1-93.9 % of viable protoplasts of C. purpureus moss were found after the treatment with studied carriers at that dose. However, at 0.25 % i.e. 100 times higher concentration than that used for moss transformation, the poly-DMAEMA carriers reached their IC 50 level. Conclusion. The novel block-like poly-DMAEMA carriers were effective in transformation of C. purpureus moss protoplasts and demonstrated low toxicity. K e y w o r d s: polymeric carrier, poly(2-dimethylamino)ethyl methacrylate, moss Ceratodon purpureus, protoplasts transformation, toxicity.

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Section: Introductionmentioning

confidence: 99%

“…The fluoro-substituents are widely used in drug design [1,[15][16][17][18]. Moreover, the introduction of a trifluoromethyl group into drugs might improve their bioavailability, lipophilicity, metabolic stability, and promotes transport and absorption [15,18,19]. Koppireddi et al reported that 3-(3-trifluoromethylphenyl)-6-phenylimidazo[2,1-b]thiazole possessed higher anti-proliferative properties towards HeLa cells among other 3,6-diphenyl imi dazo[2,1-b]thiazole derivatives [18].…”

Section: Introductionmentioning

confidence: 99%

Study in vitro of the anticancer activity of [3-allyl-4-(41-methoxyphenyl)-3H-thiazole-2-ylidene]-(32-trifluoromethylphenyl)amine hydrobromide toward human tumor cells

et al. 2022

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In vitro study and characterization of [the] anticancer activity of heterocyclic derivative -[3-allyl-4-(4 1 -methoxyphenyl)-3H-thiazole-2-ylidene]-(3 2 -trifluoromethylphenyl)amine hydrobromide. Methods. The cell culture; MTT assay. Results. We synthesized [3-allyl-4-(4 1methoxyphenyl)-3H-thiazole-2-ylidene]-(3 2 -trifluoromethylphenyl)amine hydrobromide, which possessed the cardioprotective, as well as the hypolipidemic, anti-inflammatory, analgesic, antihypertensive and antioxidant effects. Here, we investigated its growth inhibitory action towards tumor cell lines of various tissue origin[s]: leukemia (HL-60, Jurkat), liver (HepG2), breast (MCF-7), lung (A549), cervical (KB3-1) and glioma (U251, U373, T98G) cells. We found that the leukemia cells were the most sensitive to the action of [3-allyl-4-(4 1methoxyphenyl)-3H-thiazole-2-ylidene]-(3 2 -trifluoromethylphenyl)amine hydrobromide with a mean of IC 50 values at 7.5-8.9 μg/mL. Conclusions. The anti-proliferative activity of [3-allyl-4-(4 1 -methoxyphenyl)-3H-thiazole-2-ylidene]-(3 2 -trifluoromethylphenyl)amine hydrobromide dropped in the order: leukemia > hepatocarcinoma ~ cervix > lung carcinoma > glioblastoma > breast carcinoma cells. Thus, we revealed in one molecule of ([3-allyl-4-(4 1methoxyphenyl)-3H-thiazole-2-ylidene]-(3 2 -trifluoromethylphenyl)amine hydrobromide) a combination of both the cardioprotective and anticancer activities that is of great significance for this agent as a potent anticancer medicine. K e y w o r d s: [3-allyl-4-(4 1 -methoxyphenyl)-3H-thiazole-2-ylidene]-(3 2 -trifluoromethylphenyl)amine hydrobromide, cytotoxicity in vitro, anticancer activity.

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Polyethylenimine-functionalized cationic barley β-glucan derivatives for macrophage RAW264.7 cell-targeted gene delivery systems

Cited by 5 publications

References 50 publications

Current Designs of Polymeric Platforms Towards the Delivery of Nucleic Acids Inside the Cells with Focus on Polyethylenimine

Current Designs of Polymeric Platforms Towards the Delivery of Nucleic Acids Inside the Cells with Focus on Polyethylenimine

Transformation of the moss (Ceratodon purpureus) with plasmid DNA delivered by novel block-copolymers of the dimethylaminoethyl methacrylate

Study in vitro of the anticancer activity of [3-allyl-4-(41-methoxyphenyl)-3H-thiazole-2-ylidene]-(32-trifluoromethylphenyl)amine hydrobromide toward human tumor cells

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