Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection

McBain, Stuart C; Yiu, Humphrey H. P.; Haj, Alicia J. El; Dobson, Jon

doi:10.1039/b617402g

Cited by 137 publications

(119 citation statements)

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“…For example, Plank and co-workers synthesized magnetic particles in situ in PEI solutions [8,15,16], Hottiger and coworkers directly mixed maghemite dispersions with PEI solutions [10], whilst McBain and co-workers exploited covalent bonds to attach PEI to magnetic particles [17]. The MP-PEI prepared by these methods are all capable of enhancing gene delivery.…”

Section: Nano Researchmentioning

confidence: 99%

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

et al. 2009

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Using magnetic nanoparticles to enhance gene transfection, a recently developed technique termed magnetofection, has been shown to be a powerful technology in gene delivery. The most widely used magnetic nanoparticles in this area are those coated with polyethyleneimine, which is a well known nonviral transfection agent. In this article, we report methods to control the aggregate size of polyethyleneiminecoated magnetite particles. These particles were then used to enhance transfection of green fl uorescent protein (GFP) into NIH 3T3 cells in vitro. We find that the aggregate size of the particles has a great effect on their performance in magnetofection, with less aggregated magnetic particles being more effective in enhancing the gene transfection.

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Section: Nano Researchmentioning

confidence: 99%

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

et al. 2009

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“…[20][21][22] However, most previous studies of magnetofection have focused on transfection using high MW PEI because the efficiency of low MW PEI is comparably lower. 8,9,[11][12][13][14] Therefore, development of a high-efficiency low MW PEI-mediated magnetofection method is of considerable interest for practical applications due to its relatively low cytotoxicity. Another problem in magnetofection is using aggregated PEI-conjugated Fe 3 O 4 (PEI-Fe 3 O 4 ) nanoparticles, which are usually induced by random cross-linking between the positive PEI and anion molecules bonded to the Fe 3 O 4 nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

“…[5][6][7] To make the magnetofection more effective, the magnetic nanoparticles are usually conjugated with cationic polymers because the positive charge of the cationic polymers help form stable complexes with the negatively charged DNA via electrostatic interaction. [8][9][10] The PEI is one of the most extensively studied cationic polymers. 8,9,[11][12][13][14] It is generally believed that the positively charged amine groups in PEI increase the proton concentration in the endosome through proton pumping.…”

Section: Introductionmentioning

confidence: 99%

“…[8][9][10] The PEI is one of the most extensively studied cationic polymers. 8,9,[11][12][13][14] It is generally believed that the positively charged amine groups in PEI increase the proton concentration in the endosome through proton pumping. [15][16][17] Consequently, DNA release could be enhanced by osmotic swelling/rupture of the endosomal membrane, which is known to be a proton sponge effect.…”

Section: Introductionmentioning

confidence: 99%

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Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe₃O₄@SiO₂Nanoparticles

Jang¹,

Park²

2012

Bulletin of the Korean Chemical Society

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We successfully synthesized Fe 3 O 4 @SiO 2 nanoparticles with ultrathin silica layer of 1.0 ± 0.5 nm that polyethyleneimine (PEI) with low molecular weight of 2.0-4.0 kDa was covalently conjugated with the resulting Fe 3 O 4 @SiO 2 nanoparticles by silane coupling reaction. The PEI-Fe 3 O 4 @SiO 2 nanoparticles were further used as gene delivery vector for a human fibroblast cell (IMR-90) line. Gene transfection efficiency of the PEI-Fe 3 O 4 @SiO 2 complexes did not increase remarkably after magnetofection; however, the addition of Lipofectamine 2000 significantly increased the transfection efficiency of the PEI-Fe 3 O 4 @SiO 2 complexes. We believe that the present approach could be utilized for magnetofection as alternative to Fe 3 O 4 nanoparticles conjugated with the PEI of high molecular weight thanks to its relatively low cytotoxicity and high transfection efficiency.

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“…e "proton sponge" nature of PEI is thought to provide buering within the endosomes, improving PEI bound GNP stability a er cellular internalization [23][24][25][26][27]. Furthermore, the steric hindrance imparted by PEI surface functionalization imparts added colloidal stability for the gold in solution [27].…”

mentioning

confidence: 99%

The biodistribution and pharmacokinetic evaluation of choline-bound gold nanoparticles in a human prostate tumor xenograft model

Razzak¹,

Zhou²,

Yang³

et al. 2013

CIM

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e biodistribution and pharmacokinetic evaluation of choline-bound gold nanoparticles in a human prostate tumor xenogra model Abstract Purpose: Gold nanoparticles (GNPs) have attracted signi cant attention in the treatment of cancer due to their potential as novel radiation enhancers, particularly when functionalized with various targeting ligands. e aim of this study was to assess the biodistribution and pharmacokinetic characteristics of a novel choline-bound GNP (choline-GNP) stabilized with polyethelenimine (PEI).Methods: Choline bound to 27 nm diameter GNPs was characterized using transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). Toxicity of choline-GNPs was examined on DU-145 prostate cancer cells using an MTT assay. Using balb/c mice bearing ank DU-145 prostate tumors, choline-GNPs bio-distribution was measured using inductively coupled mass spectroscopy (ICP-MS). Blood, heart, lung, liver, spleen, brain, kidney and tumor gold content were examined at multiple time points over a 24-hour period a er tail vein injection.Results: An MTT assay using DU-145 prostate cancer cells yielded a 95% cell viability 72 hours a er choline-GNP administration. e tumor GNP area under the concentrationtime curve during the rst 4 hours (AUC0-4) was 2.2 µg/ml h, representing 13% of the circulating blood GNP concentration over the same time period. e maximum intratumor GNP concentration observed was 1.4% of the injected dose per gram of tumor tissue (%ID/g) one hour post injection.Conclusions: GNPs functionalized with choline demonstrates a viable future nanoparticle platform with increased intra-tumor uptake as compared to unconjugated GNPs. Decreased intra-hepatic accumulation appears to be the reason for the improved systemic bioavailability. e next logical translational investigation will incorporate external beam radiation with the observed maximum intra-tumor uptake.

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Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection

Cited by 137 publications

References 16 publications

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe₃O₄@SiO₂Nanoparticles

The biodistribution and pharmacokinetic evaluation of choline-bound gold nanoparticles in a human prostate tumor xenograft model

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Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection

Cited by 137 publications

References 16 publications

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe3O4@SiO2Nanoparticles

The biodistribution and pharmacokinetic evaluation of choline-bound gold nanoparticles in a human prostate tumor xenograft model

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Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe₃O₄@SiO₂Nanoparticles