Polycyclic aromatic hydrocarbons (PAHs) – Method for the determination of semi‐volatile PAHs in workplace air using high performance liquid chromatography (HPLC) [Air Monitoring Methods, 2018]

Abstract

This analytical method is a validated measurement procedure for the determination of six semi‐volatile polycyclic aromatic hydrocarbons (PAHs) from the EPA list (US Environmental Protection Agency) such as benzo[ b ]fluoranthene, benzo[ k ]fluoranthene, benzo[ a ]pyrene, dibenzo[ a,h ]anthracene, benzo[ ghi ]perylene, indeno[1,2,3‐cd]pyrene and also benzo[ e ]pyren in workplace air averaged over the sampling period after personal or stationary sampling. Sampling is performed by drawing a defined volume of air through a PTFE filter, which is inserted in a GSP sampling system. The collected PAH components are extracted with a mixture of acetonitrile/methanol and analysed by means of high performance liquid chromatography (HPLC) with fluorescence detection. The quantitative determination of the PAHs is based on calibration functions obtained by means of multiple‐point calibrations. The limit of quantification for an individual PAH is in the range from 0.0012 to 0.0043 µg/m 3 . Joint Publication of the Analytical Subcommittee of the Chemistry Board of Experts of the Expert Committee Raw Materials and Chemical Industry of the German Social Accident Insurance and the working group “Air Analyses” of the Permanent Senate Commission of the Deutsche Forschungsgemeinschaft for the Investigation of Health Hazards of Chemical Compounds in the Work Area.

The Use of Synchronous Fluorescence Technique in Environmental Investigations of Polycyclic Aromatic Hydrocarbons in Airborne Particulate Matter from an Industrial Region in Poland

“…Also high performed liquid chromatographs (HPLC method) are widely applied in these investigations (e.g., [20][21][22]). However, it is often not cost effective to apply these methods routinely to large numbers of samples.…”

Assay developments for detection of biological analytes

“…The current method of measuring PAH and odorant concentrations is through the use of analytical equipment such as the gas chromatography, high performance liquid chromatography or mass spectrometry [198][199][200][201][202]. These methods yield low limit of detection of down to 0.001 g mL -1 .…”