Polycyclic aromatic hydrocarbons (PAHs) degradation by laccase from a tropical white rot fungus Ganoderma lucidum

Punnapayak, Hunsa; Prasongsuk, Sehanat; Messner, Kurt; Danmek, Khanchai; Lotrakul, Pongtharin

doi:10.5897/ajb09.1073

Cited by 33 publications

(2 citation statements)

References 12 publications

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“…These ligninolytic enzymes generate water-soluble polar compounds after the ligninolytic compounds’ catalytic cleavage and aromatic compounds’ catalytic cleavage, which become available for fungal metabolism [ 81 ]. Punnapayak et al [ 82 ] demonstrated the degradation of 16 PAHs by the laccase enzyme produced by the white-poor fungus Ganoderma lucidum Chaaim-001 BCU. This enzyme completely degraded anthracene and benzo[a]pyrene, using 1-hydroxybenzotriazole as a redox mediator.…”

Section: Biosolid Treatmentmentioning

confidence: 99%

Enzymatic Treatments for Biosolids: An Outlook and Recent Trends

Quintero-García

Pérez-Soler

Amezcua‐Allieri

2023

IJERPH

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Wastewaters are nutrient-rich organic materials containing significant concentrations of different nutrients, dissolved and particulate matter, microorganisms, solids, heavy metals, and organic pollutants, including aromatic xenobiotics. This variety makes wastewater treatment a technological challenge. As a result of wastewater treatment, biosolids are generated. Biosolids, commonly called sewage sludge, result from treating and processing wastewater residuals. Increased biosolids, or activated sludge, from wastewater treatment is a major environmental and social problem. Therefore, sustainable and energy-efficient wastewater treatment systems must address the water crisis and environmental deterioration. Although research on wastewater has received increasing attention worldwide, the significance of biosolids treatments and valorization is still poorly understood in terms of obtaining value-added products. Hence, in this review, we established some leading technologies (physical, chemical, and biological) for biosolids pretreatment. Later, the research focuses on natural treatment by fungal enzymes to end with lignocellulosic materials and xenobiotic compounds (polyaromatic hydrocarbons) as a carbon source to obtain biobased chemicals. Finally, this review discussed some recent trends and promising renewable resources within the biorefinery approach for bio-waste conversion to value-added by-products.

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Section: Biosolid Treatmentmentioning

confidence: 99%

Enzymatic Treatments for Biosolids: An Outlook and Recent Trends

Quintero-García

Pérez-Soler

Amezcua‐Allieri

2023

IJERPH

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“…This finding was similar to that of Thongkred et al (2011) who discovered that adding ABTS to the reaction only slightly enhanced the degradation of some PAHs while decreasing the degradation of others. Trametes versicolor and Ganoderma lucidum Chaaim-001 BCU were found to be capable of degrading PAH in the absence of a redox mediator (Punnapayak et al, 2009). Another finding was revealed that without the presence of a redox mediator (ABTS), purified laccase from Trametes versicolor 95122 was unable to breakdown phenanthrene (Han et al, 2004).…”

Section: Degradation Of Pahs Using Live Mycelia and Crude Laccase Of ...mentioning

confidence: 99%

Biodegradation of polycyclic aromatic hydrocarbons commonly found in coal slurry using white rot fungi isolated from east kalimantan

Wulandari¹

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Fifty specimens of white-rot fungi were identified and placed into 6 families and 30 species. Two new records of resupinate fungi (Basidiomycota) were discovered and reported for Indonesia: Ceriporia inflata and Ceriporia lacerata. Furthermore, a four-step screening protocol to select WRF has been conducted. These stepwise protocols have selected Trametes polyzona PBURU 12 based on its superior performance among the 30 isolates tested in this experiment with laccase as the major LMEs produced. Plackett-Burman’s design was used for screening multiple factors at a time. The result suggested that the highest positive effects influencing laccase production by PBURU 12 were glucose, peptone, CuSO4 and pH. Furthermore, the response surface methodology (RSM) showed that the optimized medium induced a nearly 3-folds increase in laccase activity compared to the basal medium. The optimum pH and stability were shown at pH 4.5 and 5.0, respectively. PBURU 12 acted in the broad temperature range of 20-70°C and stable at 20-40°C for 60 minutes. Cu2+ was showed a stimulatory effect on laccase activity, while Fe2+ and NaN3 were found to be potent inhibitors. Degradation of PAHs using crude laccase (1 U/ml) of PBURU 12 was able to degrade PAHs up to 50% within 24 hours. PAHs degradation were increased to 1-fold when ABTS was added, and up to 2-fold when laccase concentration was increased to 10 U/ml. The crude laccase was capable to degrade the PAHs mixture up to 70%. The genotoxicity experiment using Allium cepa L. revealed that the laccase-treated PAHs were less toxic compared to untreated. While the results of the AMES test with Salmonella typhimurium revealed that strain TA98 and TA100 showed different mutagenic activity toward untreated and treated-PAHs.

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