Polyclonal Antisera Against Estuarine Crustacean Vitellins: A Molecular Approach to Reproductive Endocrinology and Toxicology

Mysid crustaceans as standard models for the screening and testing of endocrine-disrupting chemicals

“…The production of vitellin is under direct control of peptide hormones like the 'vitellogenesis-inhibiting hormone' (VIH) and the 'vitellogenesis-stimulating hormone' (VSH). The complex hormonal regulation of vitellogenesis makes it an excellent model for studying mechanisms of hormone signaling at the cellular and molecular level (Tuberty et al, 2002). An increasing number of studies have evaluated endocrine toxicity to vitellogenesis in crustaceans (Lee and Noone, 1995;Volz and Chandler, 2004;Sanders et al, 2005).…”

Mysid crustaceans as standard models for the screening and testing of endocrine-disrupting chemicals

“…The production of vitellin is under direct control of peptide hormones like the 'vitellogenesis-inhibiting hormone' (VIH) and the 'vitellogenesis-stimulating hormone' (VSH). The complex hormonal regulation of vitellogenesis makes it an excellent model for studying mechanisms of hormone signaling at the cellular and molecular level (Tuberty et al, 2002). An increasing number of studies have evaluated endocrine toxicity to vitellogenesis in crustaceans (Lee and Noone, 1995;Volz and Chandler, 2004;Sanders et al, 2005).…”

Mysid crustaceans as potential test organisms for the evaluation of environmental endocrine disruption: A review

“…In general, few studies have been conducted on the effects of contaminants on gonadal maturation of crustaceans [8]; however, much attention has been given recently to vitellogenin, the precursor to the yolk protein vitellin in egg‐laying invertebrates and vertebrates, as an indicator of exposure to estrogenic xenobiotics [5,209–216]. Control of vitellogenesis is being studied intensively because yolk is an excellent model for studying mechanisms of hormonal control at the cellular and molecular levels [5,215]. To assess the potential adverse effects of xenobiotics on crustacean reproduction, it is important to measure accurately vitellogenin and vitellin in crustacean models (an overview of crustacean species from which vitellin, vitellogenin, or lipovitellin has been isolated or partially characterized is given by Tuberty et al [215]).…”

“…Control of vitellogenesis is being studied intensively because yolk is an excellent model for studying mechanisms of hormonal control at the cellular and molecular levels [5,215]. To assess the potential adverse effects of xenobiotics on crustacean reproduction, it is important to measure accurately vitellogenin and vitellin in crustacean models (an overview of crustacean species from which vitellin, vitellogenin, or lipovitellin has been isolated or partially characterized is given by Tuberty et al [215]). Recently, a quantitative enzyme‐linked immunosorbent assay was developed for the mysid A. bahia by using polyclonal antisera [215].…”

“…To assess the potential adverse effects of xenobiotics on crustacean reproduction, it is important to measure accurately vitellogenin and vitellin in crustacean models (an overview of crustacean species from which vitellin, vitellogenin, or lipovitellin has been isolated or partially characterized is given by Tuberty et al [215]). Recently, a quantitative enzyme‐linked immunosorbent assay was developed for the mysid A. bahia by using polyclonal antisera [215]. In addition, studies are under way to characterize and purify vitellin of the mysid N. integer (A. Ghekiere et al, Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Ghent, Belgium, unpublished data).…”

Vitellogenin and Lipovitellin from the prawn Macrobrachium borellii as hydrocarbon pollution biomarker

“…This can be largely attributed to the shortage of basic knowledge on their endocrine systems. Nevertheless, in the last few years, knowledge on the control of vitellogenesis in arthropods has been enlarged mainly in insects and crustacean, that have become appropriate models to investigate those mechanisms dealing with the hormonal control at cellular and molecular levels (Billinghurst et al, 2000;Tuberty et al, 2002).…”

Mysid crustaceans as standard models for the screening and testing of endocrine-disrupting chemicals

“…The production of vitellin is under direct control of peptide hormones like the 'vitellogenesis-inhibiting hormone' (VIH) and the 'vitellogenesis-stimulating hormone' (VSH). The complex hormonal regulation of vitellogenesis makes it an excellent model for studying mechanisms of hormone signaling at the cellular and molecular level (Tuberty et al, 2002). An increasing number of studies have evaluated endocrine toxicity to vitellogenesis in crustaceans (Lee and Noone, 1995;Volz and Chandler, 2004;Sanders et al, 2005).…”

Mysid crustaceans as potential test organisms for the evaluation of environmental endocrine disruption: A review

“…In general, few studies have been conducted on the effects of contaminants on gonadal maturation of crustaceans [8]; however, much attention has been given recently to vitellogenin, the precursor to the yolk protein vitellin in egg‐laying invertebrates and vertebrates, as an indicator of exposure to estrogenic xenobiotics [5,209–216]. Control of vitellogenesis is being studied intensively because yolk is an excellent model for studying mechanisms of hormonal control at the cellular and molecular levels [5,215]. To assess the potential adverse effects of xenobiotics on crustacean reproduction, it is important to measure accurately vitellogenin and vitellin in crustacean models (an overview of crustacean species from which vitellin, vitellogenin, or lipovitellin has been isolated or partially characterized is given by Tuberty et al [215]).…”

“…Control of vitellogenesis is being studied intensively because yolk is an excellent model for studying mechanisms of hormonal control at the cellular and molecular levels [5,215]. To assess the potential adverse effects of xenobiotics on crustacean reproduction, it is important to measure accurately vitellogenin and vitellin in crustacean models (an overview of crustacean species from which vitellin, vitellogenin, or lipovitellin has been isolated or partially characterized is given by Tuberty et al [215]). Recently, a quantitative enzyme‐linked immunosorbent assay was developed for the mysid A. bahia by using polyclonal antisera [215].…”

“…To assess the potential adverse effects of xenobiotics on crustacean reproduction, it is important to measure accurately vitellogenin and vitellin in crustacean models (an overview of crustacean species from which vitellin, vitellogenin, or lipovitellin has been isolated or partially characterized is given by Tuberty et al [215]). Recently, a quantitative enzyme‐linked immunosorbent assay was developed for the mysid A. bahia by using polyclonal antisera [215]. In addition, studies are under way to characterize and purify vitellin of the mysid N. integer (A. Ghekiere et al, Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Ghent, Belgium, unpublished data).…”

Vitellogenin and Lipovitellin from the prawn Macrobrachium borellii as hydrocarbon pollution biomarker

“…This can be largely attributed to the shortage of basic knowledge on their endocrine systems. Nevertheless, in the last few years, knowledge on the control of vitellogenesis in arthropods has been enlarged mainly in insects and crustacean, that have become appropriate models to investigate those mechanisms dealing with the hormonal control at cellular and molecular levels (Billinghurst et al, 2000;Tuberty et al, 2002).…”

Mysid crustaceans as standard models for the screening and testing of endocrine-disrupting chemicals

“…The production of vitellin is under direct control of peptide hormones like the 'vitellogenesis-inhibiting hormone' (VIH) and the 'vitellogenesis-stimulating hormone' (VSH). The complex hormonal regulation of vitellogenesis makes it an excellent model for studying mechanisms of hormone signaling at the cellular and molecular level (Tuberty et al, 2002). An increasing number of studies have evaluated endocrine toxicity to vitellogenesis in crustaceans (Lee and Noone, 1995;Volz and Chandler, 2004;Sanders et al, 2005).…”

Mysid crustaceans as potential test organisms for the evaluation of environmental endocrine disruption: A review

“…In general, few studies have been conducted on the effects of contaminants on gonadal maturation of crustaceans [8]; however, much attention has been given recently to vitellogenin, the precursor to the yolk protein vitellin in egg‐laying invertebrates and vertebrates, as an indicator of exposure to estrogenic xenobiotics [5,209–216]. Control of vitellogenesis is being studied intensively because yolk is an excellent model for studying mechanisms of hormonal control at the cellular and molecular levels [5,215]. To assess the potential adverse effects of xenobiotics on crustacean reproduction, it is important to measure accurately vitellogenin and vitellin in crustacean models (an overview of crustacean species from which vitellin, vitellogenin, or lipovitellin has been isolated or partially characterized is given by Tuberty et al [215]).…”

“…Control of vitellogenesis is being studied intensively because yolk is an excellent model for studying mechanisms of hormonal control at the cellular and molecular levels [5,215]. To assess the potential adverse effects of xenobiotics on crustacean reproduction, it is important to measure accurately vitellogenin and vitellin in crustacean models (an overview of crustacean species from which vitellin, vitellogenin, or lipovitellin has been isolated or partially characterized is given by Tuberty et al [215]). Recently, a quantitative enzyme‐linked immunosorbent assay was developed for the mysid A. bahia by using polyclonal antisera [215].…”

“…To assess the potential adverse effects of xenobiotics on crustacean reproduction, it is important to measure accurately vitellogenin and vitellin in crustacean models (an overview of crustacean species from which vitellin, vitellogenin, or lipovitellin has been isolated or partially characterized is given by Tuberty et al [215]). Recently, a quantitative enzyme‐linked immunosorbent assay was developed for the mysid A. bahia by using polyclonal antisera [215]. In addition, studies are under way to characterize and purify vitellin of the mysid N. integer (A. Ghekiere et al, Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Ghent, Belgium, unpublished data).…”

Vitellogenin and Lipovitellin from the prawn Macrobrachium borellii as hydrocarbon pollution biomarker

“…This can be largely attributed to the shortage of basic knowledge on their endocrine systems. Nevertheless, in the last few years, knowledge on the control of vitellogenesis in arthropods has been enlarged mainly in insects and crustacean, that have become appropriate models to investigate those mechanisms dealing with the hormonal control at cellular and molecular levels (Billinghurst et al, 2000;Tuberty et al, 2002).…”