Polyamines in Cell Walls of Chlorococcalean Microalgae

Abstract

Biotechnology of microalgae represents a very attractive alternative as a source of energy and substances of high value when compared with plant cultivation. Cell walls of green microalgae have an extraordinary chemical and mechanical resistance and may impede some steps in the biotechnological/industrial exploitation of algae. The aim of the present contribution was to check the presence of polyamines in the cell walls of chlorococcalean green microalgae. Polyamines are nitrogenous compounds synthesized normally in cells and may affect the properties of the cell wall. Our work included strains either forming or not forming the polymer algaenan, allowing us to conclude that algaenan is not a prerequisite for the presence of polyamines in the cell walls. Polyamines were detected in isolated cell walls of Scenedesmus obliquus, Chlorella fusca, Chlorella saccharophila, and Chlorella vulgaris. Their concentration in isolated cell walls ranged between 0.4 and 8.4 nmol/mg dry weight.

Applications of microalga Chlorella vulgaris in aquaculture

“…The algaenan makes about 33–41% of the outer wall (Burczyk et al . ). On the other hand, type III is surrounded by a single layer (inner layer), which is made up of sparsely aggregated microfibrillar (Takashi & Kenji ; Rodrigues & Bon ).…”

“…Chlorella vulgaris is reported to have higher polyamines content in the cell wall, which assist in cross‐linking of the cell wall biopolymers (Burczyk et al . ). Such polyamines may be regulating the cell wall rheology of the microalga (Martínez et al .…”

“…Trilaminar outer wall contains an acetolysis-resistant biopolymer called algaenan, the layer is dubbed as the maternal cell wall. The algaenan makes about 33-41% of the outer wall (Burczyk et al 2014). On the other hand, type III is surrounded by a single layer (inner layer), which is made up of sparsely aggregated microfibrillar (Takashi & Kenji 1982;Rodrigues & Bon 2011).…”

“…It is worth mentioning that composition of polysaccharide which are the building blocks of C. vulgaris cell wall is quite dynamic, as it might be a combination of different sugars; mannose and fucose, galactose and fucose or galactose and xylose (Rodrigues & Bon 2011). Chlorella vulgaris is reported to have higher polyamines content in the cell wall, which assist in cross-linking of the cell wall biopolymers (Burczyk et al 2014). Such polyamines may be regulating the cell wall rheology of the microalga (Mart ınez et al 1991;Safi et al 2014a).…”

Cell Wall Structure of Coccoid Green Algae as an Important Trade-Off Between Biotic Interference Mechanisms and Multidimensional Cell Growth

“…Coccoid green algae have two different outer cell wall structure types with regard to chemical resistance ( Burczyk et al, 1995 ). Burczyk et al (2014) could clearly differentiate between taxa of low and high chemical resistance. HR-cell wall structures were found in several species of the genera Chlorella, Scenedesmus , and Botryococcus ( Allard and Templier, 2000 ; Scholz et al, 2014 ).…”