Polyamines and psoriasis

Voorhees, J. J.

doi:10.1001/archderm.115.8.943

Cited by 9 publications

(5 citation statements)

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“…These amines are polycationic and are elevated in actively proliferating cells. Various investigators have demonstated elevated levels of polyamine in the skin of patients with psoriasis (Voorhees, 1979). Glucocorticoids, which are potent inhibitors of polyamine synthesis, reduce polyamine levels of psoriasic skin to normal (Russell et ai, 1978).…”

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confidence: 99%

Distribution of polyamines in human epidermis

Pe¹,

Milano

Verrando³

et al. 1985

Br J Dermatol

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Consecutive sections through the epidermis were cut parallel to its surface, and the polyamine (PA) concentration in each section was measured by liquid chromatography. There was a constant decrease in PA concentration (expressed as nmol/mg DNA) from the deepest layer to the more superficial layers. Putrescine showed the greatest increase (+83%) (P less than 0.02). The elevations of spermidine and spermine levels were less marked, respectively +30% (P less than 0.05) and +27% (NS). Proliferating cells in the basal layer possess high polyamine levels and as they mature in the superficial layers their polyamine content decreases.

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confidence: 99%

Distribution of polyamines in human epidermis

Pe¹,

Milano

Verrando³

et al. 1985

Br J Dermatol

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“…The statistical data corresponding to these results are reported in table II. Much lower results were obtained for the level before incubation of cAMP and cGMP by grinding in liquid nitrogen and homogenizing in TCA ( Voorhees' [1979] method). These levels are, respectively, 4 and 0.35 pmol/mg for cAMP and cGMP in normal epidermis.…”

Section: Resultsmentioning

confidence: 96%

“…Immunological abnor malities comprise humoral [Guilhou et al, 1976a and cellular [Cormane et al, 1976;Guilhou et al, 1976b] immunity. Modifications of agents of biochemical regulation have been implicated in several ways: imbalance of prostaglandins [Hammarstr 'om et al, 1975;Michel et al, 1980], polyamines [Bohlen et al, 1978;Voorhees, 1979] and cyclic nucle otides. Concerning the latter compounds, several studies suggest a control of cellular division by the antagonist action of cyclic 3':5'-adenosine…”

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confidence: 99%

Cyclic AMP and Cyclic GMP Production in Normal and Psoriatic Epidermis

Royer

Chaintreul²,

Meynadier

et al. 1982

Dermatology

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cAMP and cGMP production was determined in normal and psoriatic (involved or uninvolved) epidermis. After homogenization, epidermal strips were incubated with saturating concentrations of ATP or GTP, respectively, for cAMP and cGMP production. Cyclic nucleotides were measured by radioimmunoassay before and after 5, 10,20 min of incubation. The kinetics of cAMP and cGMP production were linear during the 20 min of incubation. Comparison of normal skin, uninvolved and involved psoriatic skin, prior to and after 20 min of incubation showed the following results: cAMP levels were significantly higher in normal skin than in psoriatic skin at zero time (p < 0.01) and after incubation (p < 0.05). cGMP levels were not significantly different in the three tissues at zero time. After incubation, psoriatic involved epidermis exhibited a higher production of cGMP than normal skin (p < 0.05). Thus, the ratio cAMP/cGMP was higher in normal skin than in psoriatic skin at the basic level (p < 0.05) and after incubation (p < 0.01). These results indicate an imbalance in cyclic nucleotides metabolism in psoriatic skin without a significant difference between uninvolved and involved epidermis.

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“…Retinoids can inhibit the expression of ODC activity, and one proposed mechanism of action of the retinoids is by their specific inhibition of the induction of ODC activity (Haddox & Russell, 1979;Voorhees, 1979). We have measured the ability of different retinoids, applied topically to mouse skin shortly before tape stripping, to inhibit the induction of ODC activity.…”

Section: Assays For Retinoids Modulation Of Ornithine Decarboxylase Amentioning

confidence: 99%

Animal assays for anti-psoriatic., retinoid and sunprotective agents

et al. 1984

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It is possible to evaluate different dermatological therapeutic agents intended for human use in a variety of animal assays. This review will discuss some of these assays, and attempt to correlate animal and human skin responses. Psoriasis is a disease where changes in epidermal proliferation may be an important factor. It is possible to assay potential anti-psoriatic agents by measuring their ability to suppress DNA synthesis in the epidermis of hairless mice. This assay is predictive of the anti-psoriatic effectiveness of numerous agents including a variety of anti-proliferative drugs and anthralin, and has been used to evaluate the potential efficacy of purified coal tar shampoos and body preparations. The activity of the polyamine biosynthesis enzyme ornithine decarboxylase (ODC) is elevated in psoriatic skin, and it is induced in mouse epidermis by tape stripping. Retinoids can inhibit the induction of ODC activity, and this inhibition may be used to evaluate novel synthetic retinoids. Retinoids have beneficial effects on the abnormal keratinization found in various diseases. Rhino mice have multiple keratin-filled epidermal utricles, and the size of these is reduced by retinoid treatment. Observing the changes in the size of the utricles can be utilized to evaluate the effects of retinoids on keratinization. Sunscreen agents are tested on human volunteers by observing their ability to inhibit the erythema induced by exposure to solar-simulated light, to obtain a sun protection factor (SPF). It is possible to utilize the ability of sunscreens to inhibit other actinic-induced changes in the skin using animals. Parameters that may be measured include changes in DNA synthesis and ODC activity in the epidermis following ultraviolet irradiation. Some of these assays correlate well with human SPF determinations.

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Polyamines and psoriasis

Cited by 9 publications

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Distribution of polyamines in human epidermis

Distribution of polyamines in human epidermis

Cyclic AMP and Cyclic GMP Production in Normal and Psoriatic Epidermis

Animal assays for anti-psoriatic., retinoid and sunprotective agents

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