Polyacrylamide Gel Separation and Molecular Weight Determination of the Components of Cucumber Mosaic Virus RNA

Kaper, J.M.; West, Corinna

doi:10.1080/00327487208061475

Cited by 53 publications

(5 citation statements)

References 13 publications

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“…TAV was a Canadian isolate (Stace-Smith and Tremaine, 1973) and produces stunting, mild chlorosis, leaf puckering, and a reduction in apical dominance in tomato. Virions were purified from infected tomato plants and viral RNA was extracted as described (Kaper and West, 1972;Lot et al, 1972). Plants were mechanically inoculated at the cotyledon stage using RNA at 10 µg•ml -1 in 0.03 M Na 2 HPO 4 or virions at 20 µg•ml -1 in water.…”

Section: Methodsmentioning

confidence: 99%

Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

McGarvey¹,

Montasser²,

Kaper³

1994

jashs

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Transgenic tomato plants (Lycopersicon esculentum Mill.) expressing cucumber mosaic virus (CMV) satellite RNA fused to a gene for β-glucuronidase were produced using Agrobacterium-mediated transformation. The R1 progeny of self-crossed R0 plants were challenge-inoculated with virion or RNA preparations of CMV or tomato aspermy virus (TAV). The transgenic plants challenged with CMV-1 showed mild disease symptoms in the first 2 weeks postchallenge followed by a decrease in symptoms, resulting in little difference between the transgenic and uninfected control group by the fourth week. Enzyme-linked immunosorbent assay results showed about a 10-fold decrease in virus accumulation in the transgenic plants compared to controls. Tolerance was evident only in plants that contained the recombinant insert and produced mature unit-length satellite RNA after CMV infection. Plants challenged with TAV showed no significant tolerance to virus-induced symptoms.

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Section: Methodsmentioning

confidence: 99%

Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

McGarvey¹,

Montasser²,

Kaper³

1994

jashs

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“…The genomic RNAs of the CMV isolates from China were prepared using the routine method of SDS-phenol extraction (Kaper &West, 1972). After precipitation with ethanol, the preparations were stored at -20°C.…”

Section: Construction Of the Mild Strains A) Preparation Of CMV Totalmentioning

confidence: 99%

Satellite RNA for the control of plant diseases caused by cucumber mosaic virus

Tien

Zhang

Qiu

et al. 1987

Annals of Applied Biology

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AND GUSUI wu SUMMARY Two virus-protecting strains, S,, and S5?, were obtained for the control of cucumber mosaic virus (CMV) by local lesion selection after adding satellite RNA to the RNA genome of CMV. Both were found to protect pepper plants against a virulent strain of CMV under greenhouse and field conditions. Results from 14 localities in China indicated that the use of protective strains decreased the disease index by 21.6% to 82.8% and increased fruit yields by 10.8% to 55.6%. The host reactions and safety of S5, and Ss2 were tested, and the effects of the strains on plant growth were also investigated. Possible mechanisms of control of CMVcaused plant diseases by mild strains are discussed.

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“…When CMV RNA is fractionated by electrophoresis on 2•4 % polyacrylamide gels, four major and at least two minor RNA species are found (Kaper and West 1972; Peden and Symons 1973;Lot et al 1974; Lot and Kaper 1976) and these are designated RNAs 1-6 in order of decreasing molecular weight. The four major RNAs, RNAs 1-4, have molecular weights of 1• 35 x 10 6 , 1•16 X 10 6 , O' 85 X 10 6 and O' 35 x 10 6 respectively, whilst the two minor RNAs, RNAs 5 and 6, have molecular weights of about 0•12 x 10 6 and 0•05 x 10 6 respectively (Peden and Symons 1973).…”

Section: Introductionmentioning

confidence: 99%

The Two-Step Purification of Ribosomal RNA and Plant Viral RNA by Polyacrylamide Slab Gel Electrophoresis

Symons¹

1978

Aust. Jnl. Of Bio. Sci.

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The requirement for purified plant viral RNAs for sequence characterization by hybridization analysis using complementary DNA led to the development of a routine two-step procedure for their purification. The method was worked out using Escherichia coli ribosomal RNAs and then applied to four plant viral RNAs, all of which contain four major RNA components. The first of the two steps involves the electrophoresis of native RNA on 2�8 % polyacrylamide slab gels, the location of the RNA bands by brief staining and the recovery of the RNA from each gel band by electrophoretic elution. The eluted RNA, concentrated by ethanol precipitation, was then run on a second gel after a denaturation step to release nicked and aggregated RNA fragments. Each RNA band was again located by staining and recovered by electrophoretic elution and ethanol precipitation. The 16-S and 23-S ribosomal RNAs and the plant viral RNAs were obtained in overall yields of 40 and 8-16 % respectively. RNAs so purified have been successfully used for the preparation of complementary DNA by two different methods.

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Polyacrylamide Gel Separation and Molecular Weight Determination of the Components of Cucumber Mosaic Virus RNA

Cited by 53 publications

References 13 publications

Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

Satellite RNA for the control of plant diseases caused by cucumber mosaic virus

The Two-Step Purification of Ribosomal RNA and Plant Viral RNA by Polyacrylamide Slab Gel Electrophoresis

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