Poly-phenolic fraction of <i>Chrysophyllum cainito</i> extract induces cell death in osteosarcoma cells

Abstract

MaterialsGallic acid, vincristine, propidium iodide, formalin, crystal violet, crystal violet, fetal bovine serum (FBS), chloroquine, penicillin, streptomycin, 2', 7'-dichlorofluorescein diacetate, Folin-Ciocalteu's phenol reagent and annexin V-FITC apoptosis detection kit were purchased from Sigma-Aldrich. Remaining chemicals and reagents utilized in this research work were of analytical grade and were used as such. AbstractOsteosarcoma shows resistance to chemotherapy and many side effects. The ethyl acetate fraction of ethanolic extract of Chrysophyllum cainito was taken in the present work. The extract was characterized for its phenolic content, antioxidant capacity (ferric reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl assays), ability to form reactive oxygen species and annexin V assay. The extract contained phenolic content of 30.1 ± 0.2 mg GAE/g extract. Ferric reducing antioxidant power assay and DPPH assay showed 213.2 ± 12.4 mM Fe 2+ equivalent per gram extract and 85.6 ± 4.9 TE/g respectively. For production of reactive oxygen species in the osteosarcoma cells, extract at 50 µg GAE/mL concentration was statistically equivalent to 100 µM H2O2. EC50 of extract was calculated to be 133 µg GAE/mL in cell viability studies. Article Info

Chrysophyllum cainito: A Tropical Fruit with Multiple Health Benefits

“…Extracts with 25 to 200 GAE μg/mL showed major linear relations with U-2 osteosarcoma (ATCC HTB-96 ™ ) cell death, where the value of half-maximal effective concentration (EC 50 ) was 133 GAE μg/ml. e supposed mechanism of cell death was oxidative stress, Evidence-Based Complementary and Alternative Medicine leading the cells to enter apoptosis and ultimately to cell death thereafter [10].…”

“…Traditional medicinal plants play vital roles not only in disease prevention but also in treatments [4]. e biological activity of C. cainito has been researched heavily lately, which can be seen by the overall health benefits and properties of C. cainito being studied which includes but is not limited to antioxidants [5,6], antidiabetic [7,8], anti-inflammatory [9], anticancer [10], and antihypertensive properties [11]. is review is to summarize the valuable information of C. cainito where medicinal benefits have been proven.…”

Chrysophyllum cainito stem bark extract induces apoptosis in Human hepatocarcinoma HepG2 cells through ROS-mediated mitochondrial pathway

“…In traditional medicine, the leaf, fruit and stem bark of C. cainito have been used for its antidiabetes Koffi, Konan Édouard & Kouassi, 2009), antioxidant (Luo, Basile & Kennelly, 2002), anti-inflammation, anti-hypertension (Meira et al, 2014) and antibacterial activities (Oranusi, Braide & Umeze, 2015). A polyphenolic fraction from C. cainito pulp was shown to inhibit the growth of osteosarcoma cells (Li et al, 2015), indicating a potential anticancer effect.…”

“…A polyphenolic fraction from C . cainito pulp was shown to inhibit the growth of osteosarcoma cells ( Li et al, 2015 ), indicating a potential anticancer effect.…”

Comparative leaf and wood anatomical characteristics of Chrysophyllum (Sapotaceae) relate to taxonomy of the species in Thailand

“…Koffi et al (2009) claimed that alkaloids, flavonoids, phenols, sterols and triterpenes had been chemically examined in leaf of C. cainito, this has been applied to resist diabetes and rheumatoid arthritis. Nonetheless, a number of research reveal that leaf extracts of many Chysophyllum species have been used in the application of anticancer, antioxidant, antimicrobial and so on (Mallikarjun et al 2011;Meira et al 2014;Li et al 2015;Mao et al 2015;Hegde et al 2016;Ningsih et al 2016;Doan et al 2018;George et al 2018) In spite of a great number of benefits of the genus to the society, identification of Chrysophyllum has been found to be untenable. Most identification keys were constructed from their leaf and floral features (Chayamarit 2014) which are difficult for the users in identifying the plant because of the non-availability of the flowering stage and morphological similarities of a variety of species.…”