Poly(ethylene glycol)–avidin bioconjugates: suitable candidates for tumor pretargeting

Caliceti, Paolo; Chinol, Marco; Roldo, Marta; Veronese, Francesco M.; Semenzato, Alessandra; Salmaso, Stefano; Paganelli, Giovanni

doi:10.1016/s0168-3659(02)00199-2

Cited by 43 publications

(38 citation statements)

References 28 publications

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“…Modification of avidin/streptavidin by pegylation may reduce this immunogenicity. 35 Avidin/streptavidin-free cross-linking systems based on bispecific antibodies can be a more feasible option for clinical application of this induced internalization method. …”

Section: Discussionmentioning

confidence: 99%

Controlled internalization of Her-2/neu receptors by cross-linking for targeted delivery

Zhu¹,

Okollie²,

Artemov³

2007

Cancer Biology & Therapy

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Receptor mediated internalization is a crucial step for targeted intracellular delivery of therapeutic and imaging agents. It was recently demonstrated that trastuzumab, an FDA approved humanized monoclonal antibody against Her-2/neu tyrosine kinase receptor, did not induce endocytosis of the internalization resistant Her-2/neu receptor. Here we report that accelerated internalization of trastuzumab can be induced by cross-linking the cell membrane bound antibody-receptor complex with an avidin/streptavidin-biotin system. We demonstrated that internalization was achieved both in vitro and in vivo in Her-2/neu expressing human breast cancer cell lines (BT-474, SK-BR-3 and AU-565) and that repetitive labeling cycles further amplified the loading of cargo molecules within the targeted cells. No trastuzumab binding and internalization was observed in Her-2/neu negative MDA-MB-231 cells, whereas weak membrane binding and negligible internalization were detected in MCF-7 cells with low expression level of Her-2/neu receptor. The method was used to noninvasively image Her-2/neu receptors in isolated cells and in a preclinical breast cancer model with MRI. The controlled internalization of Her-2/neu receptors can potentially enhance intracellular delivery of drugs and imaging probes, and improve imaging sensitivity and selectivity as well as therapeutic efficacy, through antibody-directed binding and internalization using a pretargeting approach.

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Section: Discussionmentioning

confidence: 99%

Controlled internalization of Her-2/neu receptors by cross-linking for targeted delivery

Zhu¹,

Okollie²,

Artemov³

2007

Cancer Biology & Therapy

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“…The data in Fig. 7 show that OXavidin HABA -treated limbs exhibited a highly efficient uptake of radiolabeled biotin, both when the radiolabeled biotin was injected 16 Considering that polyethylene glycol (PEG) conjugation of avidin was previously attempted to improve its pharmacokinetic and biodistribution properties (27,46) and to investigate the extraordinary tissue binding properties of oxidized avidin, 125 I-PEG-avidin (hydrazone) was previously produced by reacting CHO groups of 125 I-oxidized avidin with a molar excess of 10-kDa PEG-hydrazide. After purification by size exclusion chromatography, 125 I-PEG-avidin was injected in the muscle of mice as described previously, and its tissue residence was compared with the one of radiolabeled avidin and oxidized avidin.…”

Section: Residency Of Oxidized Avidin In Tissue and Uptake Ofmentioning

confidence: 99%

Biochemical and Biological Characterization of a New Oxidized Avidin with Enhanced Tissue Binding Properties

Verdoliva¹,

Bellofiore²,

Rivieccio³

et al. 2010

Journal of Biological Chemistry

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Chicken avidin and bacterial streptavidin are widely employed in vitro for their capacity to bind biotin, but their pharmacokinetics and immunological properties are not always optimal, thereby limiting their use in medical treatments. Here we investigate the biochemical and biological properties of a new modified avidin, obtained by ligand-assisted sodium periodate oxidation of avidin. This method allows protection of biotin-binding sites of avidin from inactivation caused by the oxidation step and delay of avidin clearance from injected tissue by generation of aldehyde groups from avidin carbohydrate moieties. Oxidized avidin shows spectroscopic properties similar to that of native avidin, indicating that tryptophan residues are spared from oxidation damage. In strict agreement with these results, circular dichroism and isothermal titration calorimetry analyses confirm that the ligand-assisted oxidation preserves the avidin protein structure and its biotin binding capacity. In vitro cell binding and in vivo tissue residence experiments demonstrate that aldehyde groups provide oxidized avidin the property to bind cellular and interstitial protein amino groups through Schiff's base formation, resulting in a tissue half-life of 2 weeks, compared with 2 h of native avidin. In addition, the efficient uptake of the intravenously injected 111 In-Biotin-DOTA (ST2210) in the site previously treated with modified avidin underlines that tissue-bound oxidized avidin retains its biotin binding capacity in vivo. The results presented here indicate that oxidized avidin could be employed to create a stable artificial receptor in diseased tissues for the targeting of biotinylated therapeutics.

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“…Although avidin-biotin binding is an easy and straightforward method to obtain nanoparticle decorated microbubbles, it limits the in vivo application of nanoparticle loaded microbubbles due to the immunogenic nature of the avidin molecule [24]. Furthermore, the loading procedure requires several washing steps that influence microbubble stability and interbatch reproducibility.…”

Section: Drug Reservoirsmentioning

confidence: 99%

Crucial factors and emerging concepts in ultrasound-triggered drug delivery

Geers

Dewitte

Smedt

et al. 2012

Journal of Controlled Release

111

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Poly(ethylene glycol)–avidin bioconjugates: suitable candidates for tumor pretargeting

Cited by 43 publications

References 28 publications

Controlled internalization of Her-2/neu receptors by cross-linking for targeted delivery

Controlled internalization of Her-2/neu receptors by cross-linking for targeted delivery

Biochemical and Biological Characterization of a New Oxidized Avidin with Enhanced Tissue Binding Properties

Crucial factors and emerging concepts in ultrasound-triggered drug delivery

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