Pollination control technologies for hybrid breeding

Kempe, Katja; Gils, Mario

doi:10.1007/s11032-011-9555-0

Cited by 102 publications

(66 citation statements)

References 140 publications

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“…On a production site, the intended maternal line is planted in strips alternating with strips of the intended pollen donor lines and maternal plants are sprayed with CHA, while treatment of paternal plants is strictly avoided. Hybrid seeds are then harvested from the pollinated mother plants to give rise to F 1 progeny that then displays the heterosis (hybrid vigor) effect (Kempe and Gils 2011 ). The plant growth regulator Croisor®100 (Sintofen, former Dupont-Hybrinova, Saaten Union Recherche, France) is currently the only CHA for wheat registered in Europe for commercial production (Hybridwheat 2013 ).…”

Section: Hybridization Systems In Wheatmentioning

confidence: 99%

“…Alternatively, the exploitation of transgene technologies may be promising for the establishment of hybrid wheat production systems (Kempe and Gils 2011 ;Whitford et al 2013 ). As an example, a recessive split-gene transgene system was suggested that utilizes complimentary fragments of barnase to induce male-sterility in maternal plants with simultaneously retaining pollen fertility and thus grain yield in the resulting F 1 hybrids (Gils et al 2008 ;Kempe et al 2009 ).…”

Section: Hybridization Systems In Wheatmentioning

confidence: 99%

“…Functional barnase formation in the tapetum layer of anthers and corresponding male-sterility should exclusively be effective in heterozygous plants, which can eventually serve as the mother plants in the hybrid cross. With both barnase gene fragments located on allelic chromosomal positions and thus "linked in repulsion", hybrid F 1 plants inherit only one of the barnase fragments and, as a result, remain fully fertile (Kempe and Gils 2011 ).…”

Section: Hybridization Systems In Wheatmentioning

confidence: 99%

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Hybrid Breeding in Wheat

Mette

Gils

Longin

et al. 2015

Advances in Wheat Genetics: From Genome to Field

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Despite promising superior performance, hybrid wheat currently occupies only a niche sector in commercial wheat production. However, with the recent development of practicable hybrid seed production systems, a switch from line to hybrid breeding in wheat seems realistic. Here, we discuss what consequence this may have for wheat breeding programs and provide suggestions on how quantitative genetic analysis can contribute to design optimal selection strategies.

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Section: Hybridization Systems In Wheatmentioning

confidence: 99%

Section: Hybridization Systems In Wheatmentioning

confidence: 99%

Section: Hybridization Systems In Wheatmentioning

confidence: 99%

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Hybrid Breeding in Wheat

Mette

Gils

Longin

et al. 2015

Advances in Wheat Genetics: From Genome to Field

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“…Among the limited options available, the principle of heterosis is being exploited for generating hybrids which show approximately 20-30% increase in yields as compared to the high yielding varieties along with other benefits such as improved physical stability, higher responsiveness to fertilizers and better root penetration etc. (Budar and Pelletier 2001;Kempe and Gils 2011). A prerequisite for hybrid seed production is a robust pollination control system that avoids self-fertilization.…”

Section: Introductionmentioning

confidence: 99%

Development of male sterile transgenic lines in rice by tapetum specific expression of barnase gene

et al. 2017

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The key to development of barnase-barstar transgene based hybrid seed technology is the availability of tightly regulated tapetum specific promoter, as any leaky expression of the barnase gene leads to several unintended effects. In the present study, we used two different tapetum specific promoters i.e. promoter of the RTS gene isolated from rice cultivar IR64 and the OsG6b promoter from japonica rice cultivar Hayayuki to express the barnase gene in rice transgenic lines. While viable male sterile transgenic lines could not be obtained with RTS promoter we could develop single copy male sterile lines when the barnase gene was expressed under the OsG6b promoter.Keywords Hybrid seed production, male sterile lines, barnase, barstar, tapetum specific promoter, Oryza sativa IntroductionRice is the second largest food crop after maize being grown globally. The yield of the high yielding varieties (HYVs) obtained during green revolution has reached a plateau and there is a need for newer and better ways to achieve enhanced crop production (Kropff et al. 1994). Among the limited options available, the principle of heterosis is being exploited for generating hybrids which show approximately 20-30% increase in yields as compared to the high yielding varieties along with other benefits such as improved physical stability, higher responsiveness to fertilizers and better root penetration etc. (Budar and Pelletier 2001; Kempe and Gils 2011). A prerequisite for hybrid seed production is a robust pollination control system that avoids self-fertilization. The most efficient way of promoting crosspollination in bisexual plants is by using male sterility-restorer system. Cytoplasmic male sterility (CMS) due to spontaneous mutations or from interspecific crosses have been widely exploited for hybrid production in many of agricultural and agronomical crops like maize, rice, wheat, sorghum, rye, Brassica etc. (Yuan and Fu 1995;Budar and Pelletier 2001;Harvey 2004).In case of rice, the Wild Abortive (WA) -CMS system is the only system being widely used for hybrid seed production mainly in China but also in a few other countries including India since last 50 years (Singh et al. 2015). In order to avoid a situation as witnessed in case of maize T-cytoplasm (1950-1970) The barnase-barstar transgene based hybrid seed production technology has been commercially deployed in crops like Brassica napus (Mariani et al. 1990;1992). The system utilizes the expression of barnase gene, encoding cytotoxic RNase protein in the tapetum cells to make one of the combiner's male sterile. Seed produced in F 1 hybrid is restored by expression of the barstar gene, which is also expressed in the tapetum tissue brought into the F 1 through the other combiner. In the previous work on developing male sterile lines in tobacco (Mariani et al. 1990;1992) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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“…Several prerequisites must be fulfilled for commercial hybrid seed production. First, for self-pollinating (autogamous) species, self-pollination of the "female" must be precluded to ensure that the plant will only be cross-pollinated by the chosen crossing partner (i.e., the "male" line) (2). Second, because male-sterile plants cannot be multiplied by selfing, technical solutions must be available for the multiplication of the sterile crossing partner (3).…”

mentioning

confidence: 99%

Split-gene system for hybrid wheat seed production

Kempe

Rubtsova

Gils

2014

Proc. Natl. Acad. Sci. U.S.A.

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Hybrid wheat plants are superior in yield and growth characteristics compared with their homozygous parents. The commercial production of wheat hybrids is difficult because of the inbreeding nature of wheat and the lack of a practical fertility control that enforces outcrossing. We describe a hybrid wheat system that relies on the expression of a phytotoxic barnase and provides for male sterility. The barnase coding information is divided and distributed at two loci that are located on allelic positions of the host chromosome and are therefore "linked in repulsion." Functional complementation of the loci is achieved through coexpression of the barnase fragments and intein-mediated ligation of the barnase protein fragments. This system allows for growth and maintenance of male-sterile female crossing partners, whereas the hybrids are fertile. The technology does not require fertility restorers and is based solely on the genetic modification of the female crossing partner.hybrid wheat breeding | split-gene approach | site-specific recombination | intein-mediated protein splicing

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Pollination control technologies for hybrid breeding

Cited by 102 publications

References 140 publications

Hybrid Breeding in Wheat

Hybrid Breeding in Wheat

Development of male sterile transgenic lines in rice by tapetum specific expression of barnase gene

Split-gene system for hybrid wheat seed production

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