Pollen morphology of species of Dipsacus (Dipsacaceae) in the flora of Ukraine: significance for taxonomy and spore-pollen analysis

Abstract

Pollen morphology of six species of the genus Dipsacus (Dipsacaceae) in the flora of Ukraine was studied using light and scanning electron microscopy. Pollen grains of these species are 3-porate, spheroidal, ellipsoidal or oblate-spheroidal; largesized. Their outline in equatorial view is circular or oval, in polar view circular-triangular. Pores are distinct, lolongate, circular and elliptical, with an annulus and operculum. Sculpture exine is spinate-spinulate. Additional characters of taxa of Dipsacus diagnostic at the species level for the purposes of taxonomy are the dimensions of pollen and pores, shape of pores, width of the annulus, structure of the operculum, structure of the exine, and the size and location of spines. The size of pollen and pores, shape of pores, and the width of the annulus are promising for the pollen identification in the spore-pollen analysis. The exine sculpture can be used for identification of pollen at the generic level. Palynomorphological data are partly consistent with the traditional system of genus. The significance of morphological features of pollen grains of representatives of the genus Dipsacus is more obvious at the species level. The available paleofloristic data on the participation of pollen grains of Dipsacaceae in the composition of subfossil spore-pollen spectra and palynofloras of the deposits of the Upper Pleistocene-Holocene of Ukraine are generalized. The high degree of fluctuation in participation of pollen of representatives of Dipsacus in paleofloristic material has been revealed. The generalized data allowed to substantiate the possibility and significance of the use of the presented palynomorphological data for taxa of Dipsacus of the flora of Ukraine in the practice of palynological studies of the Pleistocene and Holocene deposits.

Pollen morphology of some species of the genus Cephalaria Schrad. (Caprifoliaceae) and its significance for taxonomy

“…It is well known that morphological characteristics of pollen grains as additional diagnostic features are often used in taxonomy and in reconstructing possible phylogenetic patterns of various plant groups (Çelenk, Dirmenci, Malyer, & Bicakci, 2008; Cui, Hu, Ren, & Liu, 2020; Jacobs et al, 2011; Tsymbalyuk, Bezusko, Mosyakin, & Nitsenko, 2019; Tsymbalyuk, Bezusko, & Nitsenko, 2018; Tsymbalyuk, Mosyakin, & Nitsenko, 2019; Yildiz, Arabaci, Dirmenci, & Çelenk, 2011; etc.). Studies on pollen morphology of Сephalaria are limited.…”

“…of the flora of Iran (Mostafa, Sedigheh, & Rosa, 2017) were investigated using both light microscopy and scanning electron microscopy. Pollen morphology of some Dipsacus L. species was studied by Tsymbalyuk, Bezusko, Mosyekun & Nitsenko (2019).…”

Taxonomic significance of pollen morphology in Succisa and Succisella

“…& Schult. usually have a triporate aperture type (Clarke & Jones 1981;Tsymbalyuk et al 2018Tsymbalyuk et al , 2019, and references therein). Palynological evidence, thus, supports the phylogenetic hypothesis (Caputo et al 2004;Carlson et al 2009) that the ‛Knautieae' group is more closely related to Dipsacus and Cephalaria than to Succisa and Succisella.…”

“…Terminology used in descriptions of pollen grains mainly follows the glossary by Punt et al (2007), with some necessary minor adjustments (Tsymbalyuk et al 2018(Tsymbalyuk et al , 2019. Original and published data on quantitative pollen characters representatives of Succiseae are summarized in Table 1.…”

Morphological peculiarities of flowers, pollen grains and fruits of Valeriana tuberosa (Valerianaceae)

“…Постійні препарати пилкових зерен передані на зберігання в палінотеку Національного гербарію України KW-P (Bezusko, Tsymbalyuk, 2011). Для дослідження під сканувальним електронним мікроскопом сухі пилкові зерна фіксували 96%-м етанолом та напилювали шаром золота за стандартною методикою (Tsymbalyuk et al, 2018(Tsymbalyuk et al, , 2019a…”