Pollen composition and standardisation of analytical methods

Campos, Maria Graça; Bogdanov, Stefan; Almeida-Muradian, Lígia Bicudo de; Szczesna, T.; Mancebo, Yanina; Frigerio, Christian; Ferreira, F.A.

doi:10.1080/00218839.2008.11101443

Cited by 336 publications

(208 citation statements)

References 28 publications

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“…and Staphylococcus aureus, as described by Feás et al (2012). The values obtained for all samples were below the stated on the hygienic standards (Campos et al, 2008).…”

Section: Bee Pollen Sampling and Preparationmentioning

confidence: 88%

“…Despite its promising applications, it is important to state that in nature, BP contains 20-30% water and a water activity between 0.66 and 0.82, being a favorable matrix for the proliferation of microorganisms and to the occurrence of chemical and enzymatic reactions (Campos et al, 2008). As such, the use of conservation techniques that allow an increase in BP shelf life is a generalized practice.…”

Section: Introductionmentioning

confidence: 99%

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Effect of processing conditions on the bioactive compounds and biological properties of bee pollen

Dias

Tolentino

Pascoal

et al. 2016

Journal of Apicultural Research

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Bee pollen has had a valued place in traditional medicine for centuries, even though its use in modern medicine is still limited by the lack of scientific evidence. In this study, we characterized and compared nine types of bee pollen, submitted to two preservation methods (lyophilization and drying), in relation to the content of phenolic compound and total flavonoids. The biological properties were also evaluated and compared. Pollen analysis allowed us to classify two samples as heterofloral, whilst the remaining were monofloral. The concentrations of flavonoid and phenolic compounds were greater in the lyophilized samples. Even though all samples presented antimicrobial activity, the lyophilized ones induced stronger inhibition against both Gram-negative and Gram-positive bacteria. For the yeasts studied, the difference between the two preservation procedures was not so relevant. The antioxidant activity was also greater in the lyophilized samples. Antimutagenic capacity was observed in all samples, independently of the preservation method, even though some bee pollen types decreased the number of gene conversion and mutant colonies more efficiently. In conclusion, the results suggest that lyophilization is better to preserve the bioactive compounds and biological properties of this natural product.Efecto de las condiciones de procesado sobre los compuestos bioactivos y las propiedades bioló gicas del polen de abeja El polen de abeja ha tenido un lugar valioso en la medicina tradicional durante siglos, aunque su uso en la medicina moderna sigue siendo limitado por la falta de evidencia científica. En este estudio se caracterizaron y compararon nueve tipos de polen de abeja, sometidos a dos métodos de conservació n (liofilizació n y secado), en relació n con el contenido de compuestos fenó licos y flavonoides totales. Las propiedades bioló gicas también fueron evaluadas y comparadas. El análisis del polen nos permitió clasificar dos muestras como heteroflorales, mientras que las restantes fueron monoflorales. Las concentraciones de flavonoides y compuestos fenó licos fueron mayores en las muestras liofilizadas. A pesar de que todas las muestras presentaron actividad antimicrobiana, las liofilizadas indujeron una inhibició n más fuerte contra las bacterias Gram-negativas y Gram-positivas. Para las levaduras estudiadas, la diferencia entre los dos procedimientos de conservació n no fue tan relevante. La actividad antioxidante fue también mayor en las muestras liofilizadas. Se observó capacidad antimutagénica en todas las muestras, independientemente del método de preservació n, aunque algunos tipos de polen de abeja redujeron el número de conversió n de genes y de colonias mutantes de manera más eficiente. En conclusió n, los resultados sugieren que la liofilizació n es mejor para conservar los compuestos bioactivos y las propiedades bioló gicas de este producto natural.

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“…and Staphylococcus aureus, as described by Feás et al (2012). The values obtained for all samples were below the stated on the hygienic standards (Campos et al, 2008).…”

Section: Bee Pollen Sampling and Preparationmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Effect of processing conditions on the bioactive compounds and biological properties of bee pollen

Dias

Tolentino

Pascoal

et al. 2016

Journal of Apicultural Research

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“…Indeed, several studies ascertain the pharmacological properties of this product including antioxidant, antimicrobial, antiviral, anti-inflammatory, antimutagenic, hepatoprotective and antiallergenic, that have been linked in great extent to the content in phenolic compounds (Campos et al, 2008;Feás, Vázquez-Tato, Estevinho, Seijas, & Iglesias, 2012;Pascoal, Rodrigues, Teixeira, Feás, & Estevinho, 2014).…”

Section: Introductionmentioning

confidence: 99%

Application of FTIR-ATR spectroscopy on the bee pollen characterization

Anjos¹,

Santos²,

Dias

et al. 2017

Journal of Apicultural Research

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Bee pollen contains almost all nutrients required by the human organism as well as diverse health-promoting substances. However, its composition and nutritional value greatly depend on the botanical origin. As such, it is important to develop a rapid and non-expensive methodology that allows studying its characteristics, making labelling more objective and easier. The FTIR-ATR technique was used to predict some nutritional parameters in 126 bee pollen samples. FTIR-ATR spectrum obtained in the region between 4000 and 400 cm −1 with PLS Regression models were used to correlate spectral information with the data obtained using reference methods. In this first approach with pollen samples, good correlation models with appropriate accuracy were obtained for the evaluated parameters with r 2 varying from 74.8 to 97% and residual prediction deviation between 2.0 and 5.8. These results suggest that FTIR-ATR may be a useful technique for assessing bee pollen's composition.Aplicació n de la espectroscopía FTIR-ATR a la caracterizació n del polen de abeja El polen de abeja contiene casi todos los nutrientes requeridos por el organismo humano, así como diversas sustancias promotoras de la salud. Sin embargo, su composició n y valor nutricional dependen en gran medida del origen botánico. Como tal, es importante desarrollar una metodología rápida y econó mica que permita estudiar sus características, haciendo el etiquetado más objetivo y más fácil. La técnica FTIR-ATR se utilizó para predecir algunos parámetros nutricionales en 126 muestras de polen de abeja. Se utilizó el espectro FTIR-ATR obtenido en la regió n entre 4000 y 400 cm-1 con modelos de regresió n PLS para correlacionar informació n espectral con los datos obtenidos usando métodos de referencia. En este primer abordaje con muestras de polen, se obtuvieron buenos modelos de correlació n con una precisió n adecuada para los parámetros evaluados con r2 variando de 74,8% a 97% y una desviació n de predicció n residual entre 2,0 y 5,8. Estos resultados sugieren que el FTIR-ATR puede ser una técnica útil para evaluar la composició n del polen de abeja.

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“…The physicochemical parameters were within the standard ranges of previously analysed pollen samples without any obvious outliers (Bogdanov, 2004;Carpes et al, 2007;Szczęsna, 2007;Campos et al, 2008;Mărghitaş et al, 2009), guaranteeing the freshness and high quality of the used pollen. Sample characterisation was completed by fatty acid profiles, carotenoid spectrum, and total carotenoid content (previously published in Mărgăoan et al, 2014).…”

Section: Discussionmentioning

confidence: 83%

Antiproliferative activity and apoptotic effects of Filipendula ulmaria pollen against C26 mice colon tumour cells

Mărgăoan

Zăhan

Mărghitaș

et al. 2016

Journal of Apicultural Science

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A b s t r a c t Honeybee collected pollen exhibits high nutritional and pharmaceutical benefits for the human diet and medicine. Pollen's antioxidant, anti-ageing, anti-inflammatory, anti-atherosclerosis, and cardioprotective activity, depending on the floral origin, are well known. Recent studies proposed that pollen may also be an excellent cancer-fighting candidate, as pollen harbours high amounts of phenolic substances. In our study, Filipendula ulmaria pollen (bee collected) was methanol-water extracted and used to verify its in vitro pharmacological activities on C26 mice cancer tumour cells. Three different concentrations of the extract were tested in antitumour assays. Monitoring was done after 6, 12, 24, and 48 hours. Promising results were obtained for antiproliferative and apoptotic activity of the pollen extracts, with high efficiency for the highest concentration (1 mg/mL). For both activities, time and concentration-dependent effects were observed. Pollen extracts or bee collected pollen has a high potential as an antitumour agent for use in human medicine, because they are both rich in bioactive compounds.

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Pollen composition and standardisation of analytical methods

Cited by 336 publications

References 28 publications

Effect of processing conditions on the bioactive compounds and biological properties of bee pollen

Effect of processing conditions on the bioactive compounds and biological properties of bee pollen

Application of FTIR-ATR spectroscopy on the bee pollen characterization

Antiproliferative activity and apoptotic effects of Filipendula ulmaria pollen against C26 mice colon tumour cells

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