Pollen characters as taxonomic evidence in some species of Dipsacaceae from Iran

Abstract

Pollen morphology of nine species representing four genera: Cephalaria Schrad, Dipsacus L., Pterocephalus Vaill. and Scabiosa L. of the family Dipsacaceae in Iran has been investigated by means of scanning electron microscopy (SEM). The results showed that pollen grains were triporate and tricolpate. The pollen type of Scabiosa rotata Bieb. (tri-and tetraporate) is the first report in the world. The sizes of pollen grains fall into the classification group magna (pollen grain diameter 50-100 µm). Pollen shapes vary from preoblate to prolate and their polar views were triangulate and lobate. The exine ornamentation varies from gemmate in S. rotata to spinulate in the rest studied species. Species of Scabiosa have been dispersed in UPGMA tree that this confirmed the previous studies about taxonomic problems and species complexity in this genus. These results show the transfer of the some Scabisoa species to Lomelosia Raf. based on palynological characters. Pollen morphology of the family is helpful at the generic and specific level.

Pollen morphology of some species of the genus Cephalaria Schrad. (Caprifoliaceae) and its significance for taxonomy

“…Pollen sizes of C. aytachii are given by Göktürk and Sümbül (2003) as P = 99.48 μm, E = 92.30 μm. Pollen sizes of C. kotschyi and C. procera are given by Mostafa et al (2017) as P = 57.52 μm, E = 86.89 μm and P = 55.73 μm, E = 70.05 μm, respectively. Pollen sizes of C. alpina ( P = E = 110 μm) and C. acaulis ( P = 95 μm, E = 85 μm) are given by Erdtman (1952).…”

“…& Schult. ( P = 90.43 μm, E = 74.59 μm) were investigated by Vinokurova (1959) and Mostafa et al (2017). Also the pollen grains of five species of the genus Dipsacus were studied by Clarke and Jones (1981).…”

“…The outline of the pollen grains in C. leucantha 2.6-3.3 1.6-1.8 0.6-0.7 0.6-0.7 C. syriaca 1.0-1.2 0.7-0.8 0.3 0.3-0.4 C. transsylvanica 2.0-2.3 1.5-1.7 0.4-0.5 0.4-0.7 C. demetrii 1.9-2.6 1.4-1.7 0.4-0.5 0.4-0.6 C. coriacea 2.2-2.7 1.5-2.2 0.3-0.4 0.4-0.5 C. media 1.4-1.7 1.1-1.3 0.3-0.4 0.3-0.4 C. uralensis 1.9-2.6 1.4-1.7 0.4-0.5 0.5-0.6 C. alpina 1.6-2.0 1.3-1.4 0.4-0.6 0.5-0.6 C. aristata 1.3-1.7 1.2-1. (P = 90.43 μm, E = 74.59 μm) were investigated by Vinokurova (1959) and Mostafa et al (2017). Also the pollen grains of five species of the genus Dipsacus were studied by Clarke and Jones (1981).…”

“…& Avé‐Lall. of the flora of Iran (Mostafa, Sedigheh, & Rosa, 2017) were investigated using both light microscopy and scanning electron microscopy. Pollen morphology of some Dipsacus L. species was studied by Tsymbalyuk, Bezusko, Mosyekun & Nitsenko (2019).…”

Pollen morphology of species of Dipsacus (Dipsacaceae) in the flora of Ukraine: significance for taxonomy and spore-pollen analysis

“…Вивчено морфологічні особливості пилкових зерен D. inermis Wall. флори Пакистану (Perveen, Qaiser, 2011) та D. strigosus флори Ірану (Mostafa et al, 2017).…”