Polarizing microscope study of a contractile nanofilament system: the Acantharian myoneme

Pennart, H. de; Houliston, Evelyn; Maro, Bernard

doi:10.1016/0248-4900(88)90012-3

Cited by 29 publications

(5 citation statements)

References 45 publications

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“…He concluded that the undulation is generated by the axoneme using ATP hydrolysis and that the retraction can be induced by Ca 2+ without any requirement for ATP. The mechanism of contraction could be explained, as shown by polarizing microscopy by Febvre et al [23] in the case of the myonemes of Acantharia, through the pitch variations of the filaments coiling.…”

Section: Discussionmentioning

confidence: 96%

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Analysis of the mechanism of dinoflagellate flagella contraction‐relaxation cycle

Cachon

Greuet

Cosson

et al. 1992

Biology of the Cell

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Dinoflagellates possess two flagella. One of them, the longitudinal flagellum, retracts from time to time in some species, such as Ceratium and Peridinium. Additional structures which run along the axoneme seem to be responsible for this particular behaviour. The retraction which is rapid (less than 60 ms) may be subdivided into several steps: i) the undulating movement stops; ii) the flagellum appears then as a jagged line during 20 ms; iii) finally a rapid retraction (20 ms) takes place, the flagellum being folded 20 times inside the cylindrical flagellar pocket. The measurements on video-records suggest that the R-fibre shortens to 30°7o of its original length. The contraction and relaxation mechanism of nanofilaments is proposed to be through coiling and uncoiling dependent on Ca 2+ concentration. protist / flagellar contraction / nanofilaments

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Section: Discussionmentioning

confidence: 96%

“…Other organelles such as the myonemes of Acantharia [19][20][21][22][23] appear similar both in their structure and behaviour. They are able to shorten to 3 0 -4 0 % of their length within 10-15 ms.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the mechanism of dinoflagellate flagella contraction‐relaxation cycle

Cachon

Greuet

Cosson

et al. 1992

Biology of the Cell

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“…ac-α-tubulin is present in various microtubule structures, including primary cilia, centrioles, and the mitotic spindle, and may have a role in stabilizing these structures [ 30 ]. In addition, ac-tubulin has been found in oocytes [ 31 , 32 ]. Our data showed that specific KD of Kif4a reduced acetylation of tubulin in oocytes.…”

Section: Discussionmentioning

confidence: 99%

Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes

Tang¹,

Pan²,

Lu³

et al. 2018

Aging and disease

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Kif4a, a member of the kinesin superfamily, has been reported to participate in a series of cellular processes such as chromosome condensation and cytokinesis during mitosis. However, the roles of KIF4a in meiosis are still unknown. In present study we found that the Kif4a protein expression decreased in maternal aged mouse oocytes. We then explored the roles of Kif4a in mouse oocyte meiosis by knockdown analysis. Kif4a was enriched at the spindle during mouse oocyte maturation. By specific knock down of the Kif4a using morpholino microinjection, we found that the disruption of Kif4a caused the failure of polar body extrusion. Further analysis indicated that Kif4a might affect the spindle morphology and chromosome alignment in the mouse oocytes, and this might be due to the regulation of tubulin acetylation. Moreover, our results showed that an increased proportion of aneuploidy in the Kif4a knock down oocytes, and this might be due to the loss of kinetochore-microtubule attachment. Taken together, these results suggested that Kif4a possibly regulated mouse oocyte meiosis through its effects on the spindle organization and accurate chromosome segregation, and the loss of Kif4a might be related with aneuploidy of aging oocytes.

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“…Even, SIRT2 has been found on the spindle, however, in contrast to our observation, SIRT2 occupies uniquely metaphase I spindle [48]. Surprisingly, the deacetylating action of HDACs on the spindle tubulin is considered a phenomenon essential for metaphase II spindle assembly in oocytes [44, 46], although tubulin acetylation is a marker of stable microtubules [49]. On the other hand, SIRT1 may contribute to microtubule polymerisation via alternative PLK1 regulation [50] through equal localisation on the oocyte spindle.…”

Section: Discussionmentioning

confidence: 64%

Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression

Nevoral

Landsmann

Štiavnická

et al. 2019

J Animal Sci Biotechnol

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Background SIRT1 histone deacetylase acts on many epigenetic and non-epigenetic targets. It is thought that SIRT1 is involved in oocyte maturation; therefore, the importance of the ooplasmic SIRT1 pool for the further fate of mature oocytes has been strongly suggested. We hypothesised that SIRT1 plays the role of a signalling molecule in mature oocytes through selected epigenetic and non-epigenetic regulation. Results We observed SIRT1 re-localisation in mature oocytes and its association with spindle microtubules. In mature oocytes, SIRT1 distribution shows a spindle-like pattern, and spindle-specific SIRT1 action decreases α-tubulin acetylation. Based on the observation of the histone code in immature and mature oocytes, we suggest that SIRT1 is mostly predestined for an epigenetic mode of action in the germinal vesicles (GVs) of immature oocytes. Accordingly, BML-278-driven trimethylation of lysine K9 in histone H3 in mature oocytes is considered to be a result of GV epigenetic transformation. Conclusions Taken together, our observations point out the dual spatiotemporal SIRT1 action in oocytes, which can be readily switched from the epigenetic to non-epigenetic mode of action depending on the progress of meiosis. Electronic supplementary material The online version of this article (10.1186/s40104-019-0372-3) contains supplementary material, which is available to authorized users.

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Polarizing microscope study of a contractile nanofilament system: the Acantharian myoneme

Cited by 29 publications

References 45 publications

Analysis of the mechanism of dinoflagellate flagella contraction‐relaxation cycle

Analysis of the mechanism of dinoflagellate flagella contraction‐relaxation cycle

Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes

Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression

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