Point mutations in the 23S rRNA gene of Helicobacter pylori associated with different levels of clarithromycin resistance

Abstract

Fifty-four of 59 (91.5%) clarithromycin-resistant isolates of Helicobacter pylori from different patients possessed either the A2143G (formerly A2058G) or the A2144G (formerly A2059G) mutation in the gene encoding 23S rRNA. The A2143G mutation was significantly more likely to occur in isolates with MICs exceeding 64 mg/L (65% versus 30% with the A2144G mutation; P = 0.01). The majority (26 of 31; 83.9%) of isolates with the A2143G mutation had MICs exceeding 64 mg/L. Peptic ulcer disease recurred in a substantial proportion of patients infected with H. pylori strains containing either the A2143G or the A2144G mutation.

Determinación de mutaciones de un solo nucleótido en el gen 23S rRNA de Helicobacter pylori relacionadas con resistencia a claritromicina en una población del departamento del Cauca, Colombia

“…El mecanismo de resistencia fue descubierto por primera vez por Versalovic, et al, quienes demostraron que ciertas mutaciones puntuales (adenina → guanina, adenina → citocina) en las posiciones 2142 (A2142G, A2142C) y 2143 (A2143G) (15,16) están asociadas con la pérdida de unión de la claritromicina a los ribosomas.…”

Cell Elongation and Cell Death of Helicobacter pylori Is Modulated by the Disruption of cdrA (Cell Division‐Related Gene A)

“…H. pylori becomes resistant to clarithromycin and metronidazole more frequently than amoxicillin due to point mutations of 23S rRNA (33) and rdxA (5,13,27), respectively. However, reports on amoxicillin-resistant H. pylori without β-lactamase are increasing worldwide (9,32,34), leading to a serious problem in eradication (10).…”

Clarithromycin-Susceptible and -Resistant Helicobacter pylori Isolates with Identical Randomly Amplified Polymorphic DNA-PCR Genotypes Cultured from Single Gastric Biopsy Specimens Prior to Antibiotic Therapy

“…The 23S rRNA gene is present in two copies in the H. pylori genome (12,13). In H. pylori, seven different point mutations (A21153G, G21413A, A21423G, A21423C, A21433G, A21433C, and A21423T) in the peptidyltransferase region of the V domain of the 23S rRNA gene have been found to be associated with resistance to CLR (5,9,11,12,18). In a recent study, it was found that among CLR resistant H. pylori, the predominant mutations were A21433G and A21423G, while virtually all CLR-susceptible H. pylori had no mutation in their 23S rRNAs (17).…”