Ploidy variation in multinucleate cells changes under stress

Anderson, Cori A.; Roberts, Samantha E.; Zhang, Huaiying; Kelly, Courtney M.; Kendall, A. J.; Lee, ChangHwan; Gerstenberger, John; Koenig, Aaron B.; Kabeche, Ruth; Gladfelter, Amy S.

doi:10.1091/mbc.e14-09-1375

Cited by 45 publications

(47 citation statements)

References 50 publications

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“…Septation and A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 7 nuclear division are uncoupled events in A. gossypii, and thus each hyphal compartment encloses multiple nuclei (Kaufmann and Philippsen, 2009;. These exhibit asynchronous division cycles and variable ploidy, despite sharing a common cytoplasm (Anderson et al, 2015;Gladfelter et al, 2006). At the opposite side of the germ bubble a second germ tube is formed, giving rise to a bipolar germling .…”

Section: Life Cyclementioning

confidence: 99%

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Aguiar

Silva

Domingues

2015

Biotechnology Advances

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The filamentous fungus Ashbya gossypii has been safely and successfully used for more than two decades in the commercial production of riboflavin (vitamin B2). Its industrial relevance combined with its high genetic similarity with Saccharomyces cerevisiae together promoted the accumulation of fundamental knowledge that has been efficiently converted into a significant molecular and in silico toolbox for its genetic engineering. This synergy has enabled a directed and sustained exploitation of A. gossypii as an industrial riboflavin producer. Although there is still room for optimizing riboflavin production, the recent years have seen an abundant advance in the exploration of A. gossypii for other biotechnological applications, such as the production of recombinant proteins, single cell oil and flavour compounds. Here, we will address the biotechnological potential of A. gossypii beyond riboflavin production by presenting (a) a physiological and metabolic perspective over this fungus; (b) the molecular toolbox available for its manipulation; and (c) commercial and emerging biotechnological applications for this industrially important fungus, together with the approaches adopted for its engineering.

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Section: Life Cyclementioning

confidence: 99%

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Aguiar

Silva

Domingues

2015

Biotechnology Advances

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“…Interestingly, in response to cellular stress (cell wall stress, osmotic stress, excess zinc, the antifungal fluconazole, and increased temperature), polyploid nuclei diminished and haploid nuclei predominated. These results suggest that nuclei with different ploidies are tolerated within single syncytia, and that there may be costs associated with ploidy level variation, as stress homogenizes the genome content of nuclei (63). Remarkably, ploidy reduction and homogenization of ploidy under stress is the opposite of what has been observed for the human pathogens C. albicans and C. neoformans , where stress led to genomic diversification within populations and increased aneuploidy (43, 59, 61).…”

Section: Ploidy Changes and Aneuploidy In The Context Of Experimenmentioning

confidence: 96%

“…This fungus has multiple nuclei in the same cell (syncytia) that undergo asynchronous nuclear division (139). Originally thought to be a predominantly haploid fungus, Gladfelter and colleagues recently demonstrated frequent ploidy variations for different nuclei within the same syncytium including aneuploidy (63). To identify ploidy changes, they used lac operator arrays to track the copy number of individual chromosomes within the nuclei of the same syncytia and found nuclear ploidies ranging from 1N to >4N (63).…”

Section: Ploidy Changes and Aneuploidy In The Context Of Experimenmentioning

confidence: 99%

“…Originally thought to be a predominantly haploid fungus, Gladfelter and colleagues recently demonstrated frequent ploidy variations for different nuclei within the same syncytium including aneuploidy (63). To identify ploidy changes, they used lac operator arrays to track the copy number of individual chromosomes within the nuclei of the same syncytia and found nuclear ploidies ranging from 1N to >4N (63). Interestingly, in response to cellular stress (cell wall stress, osmotic stress, excess zinc, the antifungal fluconazole, and increased temperature), polyploid nuclei diminished and haploid nuclei predominated.…”

Section: Ploidy Changes and Aneuploidy In The Context Of Experimenmentioning

confidence: 99%

“…Ashbya gossypii , a filamentous fungus, has a single syncytia with multiple nuclei, and each nucleus can have a different ploidy (63). In A. gossypii , ploidy increases with age while stress exposure can shift a population with high ploidy variation towards a more homogenous, haploid population (63). Ploidy level variation and aneuploidy are also common in isolates of the amphibian chytrid pathogen, Batrachochytrium dendrobatidis ( B.d. )…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ploidy Variation in Fungi: Polyploidy, Aneuploidy, and Genome Evolution

2017

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The ability of an organism to replicate and segregate its genome with high fidelity is vital to its survival and for the production of future generations. Errors in either of these steps (replication or segregation) can lead to a change in ploidy or chromosome number. While these drastic genome changes can be detrimental to the organism, resulting in decreased fitness, they can also provide increased fitness during periods of stress. A change in ploidy or chromosome number can fundamentally change how a cell senses and responds to its environment. Here, we discuss current ideas within fungal biology that illuminate how eukaryotic genome size variation can impact the organism at a cellular and evolutionary level. One of the most fascinating observations from the last two decades of research is that some fungi have evolved the ability to tolerate large genome size changes and generate vast genomic heterogeneity without undergoing canonical meiosis.

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The Isoflavanoid (+)‐PTC Regulates Cell‐Cycle Progression and Mitotic Spindle Assembly in a Prostate Cancer Cell Line

Farias

Rosa‐Ribeiro

Souza

et al. 2022

Chemistry & Biodiversity

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Prostate cancer is the second most common malignancy in men and the development of effective therapeutic strategies remains challenging when more advanced, androgen-independent or insensitive forms are involved. Accordingly, we have evaluated, using flow cytometry, confocal microscopy and image analysis, the antiproliferative effects of (+)-2,3,9-trimethoxypterocarpan [(+)-PTC, 1] on relevant human prostate cancer cells as well as its capacity to control mitosis within them. In particular, the studies reported herein reveal that (+)-PTC exerts anti-proliferative activity against the PC-3 cell lines by regulating cell-cycle progression with mitosis being arrested in the prophase or prometaphase. Furthermore, it emerges that treatment of the target cells with this compound results in the formation of monopolar spindles, disorganized centrosomes and extensively disrupted γ-tubulin distributions while centriole replication remains unaffected. Such effects suggest (+)-PTC should be considered as a possible therapy for androgen-insensitive/independent prostate cancer.

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Ploidy variation in multinucleate cells changes under stress

Cited by 45 publications

References 50 publications

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Ploidy Variation in Fungi: Polyploidy, Aneuploidy, and Genome Evolution

The Isoflavanoid (+)‐PTC Regulates Cell‐Cycle Progression and Mitotic Spindle Assembly in a Prostate Cancer Cell Line

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