PLFA and EL‐FAME Indicators of Microbial Community Composition

Veum, Kristen S.; Acosta-Martínez, Verónica; Lehman, R. Michael; Li, Chenhui; Cano, Amanda; Nunes, Márcio Renato

doi:10.1002/9780891189831.ch12

Cited by 2 publications

(2 citation statements)

References 86 publications

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“…Phospholipid Fatty Acids (PLFA) have several properties that make them useful as indicators of environmental stress [9]. They are key components of the microbial membrane and as such can respond to stress from both the intracellular and extracellular environment [10]. Several researchers used PLFA to evaluate the soil biomass and determine the structure of microbiota for examining the effects of agricultural fertility [11], soil pollution [12], for ecotoxicological testing [13], or inspecting the incorporation of marked substrates [14].…”

Section: Introductionmentioning

confidence: 99%

“…Several researchers used PLFA to evaluate the soil biomass and determine the structure of microbiota for examining the effects of agricultural fertility [11], soil pollution [12], for ecotoxicological testing [13], or inspecting the incorporation of marked substrates [14]. Several microbial taxa can be analyzed by their whole biomarker patterns [10,11]. PLFA pattern measurement is usually used in soil studies to measure differences between bacterial and fungal biomass.…”

Section: Introductionmentioning

confidence: 99%

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Biomonitoring and Assessment of Dumpsites Soil Using Phospholipid Fatty Acid Analysis (PLFA) Method—Evaluation of Possibilities and Limitations

et al. 2022

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Dumped waste is not only a problem from an aesthetic point of view, but also has an environmental polluting effect, or can even pose a direct danger if the waste is dumped in illegal landfills in an uncontrolled manner with unknown composition. In the case of soil pollution, the assessment of the changing microbial state can be used as an indicator of initial changes, since waste as a pollutant impacts the diversity of the landfill’s microbial community. The degree of change depends on the qualitative and quantitative composition of the pollutants, which can be measured through the microbial phospholipid fatty acid (PLFA) pattern. The aim was a comprehensive assessment of the soil microbiological and toxicological hazards of various illegal landfill. Cluster-analysis of the average principal component revealed significant differences between the experimental sites. In comparison with the control site, the percentage of fatty acid biomarkers of Gram-positive bacteria was significantly higher in the contaminated areas, as well as the ratio of trans/cis isomerization in the case of 16:1ω7 and 18:1ω7 fatty acids. The inverse tendency was observed in the relative quantities of fatty acid biomarkers of Gram-negative bacteria compared to Actinomycetes, and in the fungal-bacterial ratio.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biomonitoring and Assessment of Dumpsites Soil Using Phospholipid Fatty Acid Analysis (PLFA) Method—Evaluation of Possibilities and Limitations

et al. 2022

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Soil health influenced more by conservation tillage and cropping practice than irrigation level in a sandy semiarid cotton system

Petermann,

Lewis,

Acosta‐Martinez

et al. 2024

Soil Science Soc of Amer J

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Cropping systems in semiarid regions have frequently relied on continuous tillage and irrigation, but declining groundwater resources have prompted a greater focus on conservation practices to improve soil health and water storage. We compared soil health responses from cotton production systems in semiarid, coarse‐textured soils with different crop management strategies under high or low irrigation levels. Management systems included continuous cotton with conventional tillage (CCCT) compared to no‐till cotton with a rye cover crop (NTCR) and no‐till cotton with a wheat‐fallow rotation (NTCW), including high or low irrigation zones within each system. Samples were collected annually from two bulk soil depths (0–10 cm and 10–20 cm) and root‐associated soils 7 years after systems were established and continued for 2 years. We found that cropping system, but not irrigation level, altered soil microbial communities and other soil health indicators. Despite variation between study years and sampling zones, the conservation systems had greater soil microbial community size via ester‐linked fatty acid methyl ester (EL‐FAME or FAME) analysis, extracellular enzyme activities, and soil organic matter than the CCCT system. The NTCW system also had greater arbuscular mycorrhizal fungi FAME abundance. Our study suggests that no‐till and conservation strategies such as cover cropping and rotation can improve biological soil health indicators in these sandy semiarid soils even with limited irrigation.

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PLFA and EL‐FAME Indicators of Microbial Community Composition

Cited by 2 publications

References 86 publications

Biomonitoring and Assessment of Dumpsites Soil Using Phospholipid Fatty Acid Analysis (PLFA) Method—Evaluation of Possibilities and Limitations

Biomonitoring and Assessment of Dumpsites Soil Using Phospholipid Fatty Acid Analysis (PLFA) Method—Evaluation of Possibilities and Limitations

Soil health influenced more by conservation tillage and cropping practice than irrigation level in a sandy semiarid cotton system

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