2011
DOI: 10.1186/1750-1326-6-40
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: BackgroundPleiotrophin is known to promote the survival and differentiation of dopaminergic neurons in vitro and is up-regulated in the substantia nigra of Parkinson's disease patients. To establish whether pleiotrophin has a trophic effect on nigrostriatal dopaminergic neurons in vivo, we injected a recombinant adenovirus expressing pleiotrophin in the substantia nigra of 6-hydroxydopamine lesioned rats.ResultsThe viral vector induced pleiotrophin over-expression by astrocytes in the substantia nigra pars com… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

2
28
0

Year Published

2014
2014
2023
2023

Publication Types

Select...
4
4

Relationship

0
8

Authors

Journals

citations
Cited by 35 publications
(30 citation statements)
references
References 37 publications
2
28
0
Order By: Relevance
“…The sum of TH-ir neurons in the four nigral levels was expressed as a percentage with respect to the contralateral SNpc. The percentage of TH-ir area was determined on the lesioned striatum on every tenth 40-mm thick coronal section (a total of seven sections covering the striatum between 1.6 and À1.3 mm related to bregma) (Paxinos and Watson, 1986;Taravini et al, 2011). Optical density measurements were performed using the National Institutes of Health ImageJ software (http://rsbweb.nih.gov/ij/, NIH, USA).…”
Section: Quantificationmentioning
confidence: 99%
See 2 more Smart Citations
“…The sum of TH-ir neurons in the four nigral levels was expressed as a percentage with respect to the contralateral SNpc. The percentage of TH-ir area was determined on the lesioned striatum on every tenth 40-mm thick coronal section (a total of seven sections covering the striatum between 1.6 and À1.3 mm related to bregma) (Paxinos and Watson, 1986;Taravini et al, 2011). Optical density measurements were performed using the National Institutes of Health ImageJ software (http://rsbweb.nih.gov/ij/, NIH, USA).…”
Section: Quantificationmentioning
confidence: 99%
“…The proportion of TH-ir cells remaining in the SNpc injected with 6-OHDA or vehicle was estimated from direct counts on nigral sections using the software Mercator Pro (Explora Nova, France). TH-ir neurons were blindly counted using a 40Â objective in the ipsilateral and contralateral SNpc of four 40-mm thick coronal section (4.80, 5.30, 5.80 and 6.04 mm posterior to bregma) (Paxinos and Watson, 1986;Taravini et al, 2011). The sum of TH-ir neurons in the four nigral levels was expressed as a percentage with respect to the contralateral SNpc.…”
Section: Quantificationmentioning
confidence: 99%
See 1 more Smart Citation
“…Briefly, three categories of AIMs were observed Immunohistochemistry: From the totality of mice included into the analysis of AIMs, twenty per genotype were perfused transcardially with 4% paraformaldehyde 1 h after the last dose of L-DOPA (the remaining 10/genotype were prepared for WB). Brains were processed, and immunodetection performed in both striatum and substancia nigra as previously reported [33,34]. Briefly, 30 μm free-floating coronal sections were incubated with the following primary antibody: rabbit anti-tyrosine hydroxylase (Pel-Freez Biologicals USA Cat# P40101 RRID:AB_2313713; TH, 1/1000) and rabbit anti-FosB/ΔFosB (Santa Cruz Biotechnology Cat# sc-48 RRID:AB_631515; 1/10000).…”
Section: Dopaminergic Depletion With 6-hydroxydopamine (6-ohda)mentioning
confidence: 99%
“…The quantitative assessment of TH-positive neurons was performed essentially as described [43]. Shortly, TH immunoreactive cell bodies were counted in 3 consecutive coronal sections by an operator blind to the treatment using a ×40 objective in the ipsilateral and contralateral substantia nigra pars compacta .…”
Section: Methodsmentioning
confidence: 99%