Platelets in blood stored in untreated and siliconed glass bottles and plastic bags: I Studies in vitro

Accumulation of di-2-ethylhexyl phthalate (DEHP) in whole blood, platelet concentrates, and platelet-poor plasma. 1. Effect of DEHP on platelet survival and function.

“…Various investigators have studied the effect of plastic containers on platelet survival and function (8)(9)(10)(11). The accumulation of DEHP in the supernatant of platelet concentrates stored at 4°C and at 22°C for up to 72 hr prompted us to study the effects in vitro of the addition of DEHP to plateletrich plasma.…”

“…The widespread use of plastic bags in routine blood bank procedures has been attended by concern about the accumulation of substances from the plastic containers in the blood products and the biologic effects of these substances (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). Most poly(vinyl chloride) plastic bags contain di-2-ethylhexyl phthalate (DEHP) to make them pliable enough to prevent breakage (2,5).…”

The Use of Plastics in Blood Transfusion Equipment with Special Regard to Toxicity Problems

“…Different criteria have been used by different in vestigators in evaluating the influence of storage time, preservation solution and different containers. After some initial reports [9,13,37,43] claiming the superiority of plastic or siliconed glass containers more critical investigations have been published [5,19,20,21,26,31]. Kissmeyer-Nielsen [20] in a comparative study of the effects on thrombocytes in contact with untreated and siliconed glass and plastics found no essential differences between these materials after different periods of storage.…”

“…It is of interest to note that in the cited investigations [19,20,21,31] only one type of plastics has been used, i.e. PVC manufactured by Fenwal.…”

Chemical and Biological Test-Methods Applied to Plastic Products Used in Transfusion Equipment

“…thrombocytes [2,7,8,16], but the technical difficulties have made them unsuitable for use in standards.…”