Platelet Storage: An Assessment of the Requirements for Plasma and Oxygen

Rock, Gail; Tittley, P.; Sherring, V A; Culley, Charles M.; Wong, Shuk-Ching

doi:10.1046/j.1537-2995.1981.21281178152.x

Cited by 26 publications

(12 citation statements)

References 11 publications

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“…It is known that mitochondrial metabolism can be very sub stantial in platelets [12]. The data presented in this report does not support the recent suggestion that oxidative metabolism is of minimal importance in preventing in creased lactate formation during platelet concentrate storage [11],…”

Section: Discussioncontrasting

confidence: 96%

“…Our data empha sizes that pC02 levels overall are closely related to the pH of the plasma of a platelet concentrate. The association in stored plate lets between pC02 and pH levels has also been described in another recent report [11]. The pC02 values determined for concen trates stored for 24 h (table II) are somewhat higher than those reported in one study [1] but similar to those reported in a second study [13].…”

Section: Discussionsupporting

confidence: 82%

“…In either case, the p02 levels did not change substantially during the 72-hour storage period. The constancy of p02 levels during 72 h storage at 20-24°C storage has also been reported in a recent study by Rock et al [11]. We have found in paired experiments involving modified platelet concentrates that a substantial difference in pH was not associated with a difference in plasma p02 levels.…”

Section: Changes In Metabolic Parameters During Storagesupporting

confidence: 89%

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Factors Influencing Changes in pH during Storage of Platelet Concentrates at 20-24 °C

Moroff¹,

Friedmann²,

Robkin-Kline³

1982

Vox Sanguinis

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The magnitude of the pH change during platelet concentrate storage at 20-24 °C in polyvinyl chloride containers is not determined solely by platelet count per cubic millimeter of plasma, since a wide variation in pH was observed with similar platelet concentrations. In modified platelet concentrates having lost through centrifugation 3-15% of total platelets and 61-92% of residual leukocytes, the pH was maintained at substantially higher levels than in the paired control platelet concentrates. Leukocyte levels do not appear to determine the magnitude of the pH fall. Continuous oxygen ulilization is needed if the pH is to be maintained near 7.0. However, oxygen tension per se is not the factor which influences the extent of pH change. It has been concluded that a specific platelet subpopulation comprising a small proportion of the total platelets in concentrates and having an enhanced capacity to form lactate may be responsible for a major part of the pH reduction which occurs during storage of many platelet concentrates.

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Section: Discussioncontrasting

confidence: 96%

Section: Discussionsupporting

confidence: 82%

Section: Changes In Metabolic Parameters During Storagesupporting

confidence: 89%

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Factors Influencing Changes in pH during Storage of Platelet Concentrates at 20-24 °C

Moroff¹,

Friedmann²,

Robkin-Kline³

1982

Vox Sanguinis

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“…lower pH, higher lactate) already suggested by previous investiga tions by ourselves [5,6,12,13] and others [1,3,4] (tables for all measured responses (table IV), although only the change in serotonin uptake from 80 to 59% was statisti cally significant (p<0.001).…”

Section: Resultssupporting

confidence: 51%

5-Day Storage of Human Platelet Concentrates in 30 ml of Plasma or Artificial Medium

Adams¹,

Swenson²,

Rock³

1987

Vox Sanguinis

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Optimal conditions for the storage of platelet concentrates were studied by changing 5 environmental parameters: bag composition (PL 146 vs. PL732), volume of plasma (60 vs. 30 ml), anticoagulant (CPDA^-1 vs. heparin), nutrient (glucose vs. fructose) and medium (plasma vs. artificial medium). A full bilevel factorial study was conducted to evaluate each variable alone and in combination with the other variables for their effects on platelet aggregation and release in response to single and pairs of stimuli. Serotonin uptake, pCO(2), platelet count, lactate, glucose, pO(2), pH and white blood cell concentration were also measured after 3 and 5 days of storage. Platelets that were stored in PL146 bags had reduced responses to stimulation by 3 days and markedly impaired responses after 5 days relative to platelets that were stored in PL732 bags. There was a large drop in pH and platelet responsiveness when platelets were stored in a volume of 30 ml in PL146 bags; these were not found when platelets were stored in 30 ml in PL732 bags. Replacing plasma with an artificial medium or adding fructose or heparin and calcium to plasma yielded platelets that were equally functional as routine controls in CPD-A1 plasma. It was concluded that replacement of plasma with 60 ml of artificial medium or a reduction of plasma volume with storage in PL732 bags are two possible mechanisms of obtaining more plasma from blood donations without compromising maximum platelet storage life.

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“…

In their recent paper, Högman et al [1] have shown that when red cells were kept in an anaerobic environ ment in the presence of nitrogen, the red cell ATP and adenylate energy charge were significantly better main tained than when red cells were stored in an aerobic environment.They also reported impaired posttransfusion survival and higher spontaneous hemolysis when red cells were stored in some of the newer more permeable plastics rather than traditional polyvinyl chloride (PVC) bags.We had earlier reported similar effects during storage of platelets with impaired in vitro function when platelets were kept in an enriched aerobic environment in which additional oxygen was either added to the storage bag or present in a high concentration in the storage environ ment [2]. In contrast, platelets kept in a nitrogen environ ment -as the red cells in the experiment of Hogman et al

[1] -showed a better in vitro function at all periods of storage.

…”

mentioning

confidence: 65%