Platelet-rich plasma loaded hydrogel scaffold enhances chondrogenic differentiation and maturation with up-regulation of CB1 and CB2

The Addition of Platelet-Rich Plasma to Scaffolds Used for Cartilage Repair: A Review of Human and Animal Studies

“…The types of scaffolds used in the eligible studies varied from malleable gel-like plugs [57][58][59][60][61][62][63][64] to solid scaffolds [65][66][67] or bone-or cartilage-mimicking inserts [68][69][70][71][72][73][74][75][76] (Tables 1 and 2). One study used a tissue-engineered cartilage construct as the implant, 77 66 The use of cell-seeded scaffolds with PRP provided favorable results because 80% of these studies (8 of 10) showed positive regenerative outcomes (structural and biochemical) when using this strategy for cartilage repair.…”

“…69 Of the 12 studies, 5 showed an improved appearance with PRP 59-61,65,66 whereas 6 reported no difference from control 57,67,68,70,71,77 (Tables 1 and 4). Some studies were able to see less prominent sites of integration between the implanted scaffold and native tissue, 58,60,62,65 whereas distinct margins were usually seen in control groups. Histologic analysis was performed in all studies except one 58 and commonly showed significantly improved repair cartilage in the presence of PRP (8 of 12 studies) 57,[59][60][61][65][66][67]70 ( Tables 1 and 4).…”

“…Some studies were able to see less prominent sites of integration between the implanted scaffold and native tissue, 58,60,62,65 whereas distinct margins were usually seen in control groups. Histologic analysis was performed in all studies except one 58 and commonly showed significantly improved repair cartilage in the presence of PRP (8 of 12 studies) 57,[59][60][61][65][66][67]70 ( Tables 1 and 4). Histologic samples of the repair cartilage were quantitatively analyzed using the modified O'Driscoll histologic score scale, 80 ICRS histologic scale, 54 or Niederauer histologic score.…”

Regenerative and innovative joint medication in the horse – Part 2: Efficacy and adverse effects of joint medication in the horse - A review of the literature - The pathogenesis of osteoarthritis in the horse

“…Das so gewonnene PRP enthält 2-8 mal mehr Thrombozyten/µl als natives Blutplasma (1,1x10 6 Thrombozyten/µl) und wird zur Behandlung von Wunden, Tendopathien, knöchernen Defekten oder OA eingesetzt (Gonshor 2002, Miller et al 2007, Textor 2011, Anitua et al 2012. Eine Vielzahl von Wachstumsfaktoren wie insulin-like growth factor 1 und 2 (IGF-1/2), epidermal growth factor (EGF) oder connective tissue growth factor (CTGF) werden von den a-Granula der Thrombozyten ausgeschüttet (Marx 2004, Blair und Flaumenhaft 2009 Anhand von In vitro-Untersuchungen, durchgeführt mit Chondrozyten von Schweinen und Kaninchen sowie humanen Stammzellen konnte gezeigt werden, dass PRP die Zellproliferation sowie die Synthese von Proteoglykan und Kollagen Typ II fördert (Akeda et al 2006, Mishra et al 2009, Lee et al 2012. Es liegen derzeit jedoch noch keine wissenschaftlichen Studien zur Behandlung der OA des Pferdes mit PRP vor.…”

Long-term stable hydrogels for biorelevant dissolution testing of drug-eluting stents

“…They are used for cell encapsulation [8], tissue engineering [9] and in controlled release dosage forms [10,11]. Recently, hydrogels have been used for biorelevant dissolution testing of dosage forms which are injected or implanted into tissue [12][13][14][15][16] to simulate some properties of the tissue surrounding the drug delivery systems such as drug transport via diffusion.…”