“…Ann-Kathrin Börger, 1,5* Dorothee Eicke, 1,5* Christina Wolf, 1 Christiane Gras, 1,5 Susanne Aufderbeck, 1 Kai Schulze, 2 Lena Engels, 3,5 Britta Eiz-Vesper, 1 Axel Schambach, 4,5 Carlos A Guzman, 2,5 Nico Lachmann, 4,5 Thomas Moritz, 4,5 Ulrich Martin, 3,5 Rainer Blasczyk, 1,5 and Constança Figueiredo iPSCs were seeded on Laminin-521-coated plates in StemMACS medium. On d 0, the medium was replaced by StemMACS and APEL medium (STEMCELL Technologies; 1:1, vol:vol) containing vascular endothelial growth factor (VEGF; 50 ng/mL) and bone morphogenetic protein 4 (BMP4; 50 ng/mL).…”