Platelet function of whole blood after short‐term cold storage: A prospective in vitro observational study

Kusudo, Eriko; Murata, Yutaka; Matsumoto, Tsuguhiro; Kawamoto, Shuji; Egi, Moritoki

doi:10.1111/trf.17216

Cited by 4 publications

(3 citation statements)

References 39 publications

(62 reference statements)

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“…It was found that platelets released approximately 50% β‐TG when stored at room temperature for more than 5 days [30]. Cold storage of whole blood for 6 h will cause platelet aggregation and the release of more β‐TG and PF4 [31]. The reason for the reduction in β‐TG release in the 24‐PTW group was thought to be because the β‐TG release from frozen platelets decreases along with the reduction in the activation function of platelets.…”

Section: Discussionmentioning

confidence: 99%

Cryopreserved platelets washed with a dialysis machine for dimethyl sulphoxide removal

Huang

Lin

et al. 2023

Vox Sanguinis

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Background and ObjectivesCryopreserved platelets (cPLTs) can be stored for years and are mainly used in military settings. However, the commonly used cryoprotectant dimethyl sulphoxide (DMSO) has toxic side effects when utilized in high quantities. We developed a novel method to aseptically remove DMSO from thawed cPLTs by dialysis.Materials and MethodsOne unit of platelets (N = 6) was mixed with 75 mL of 27% DMSO within 4 days after collection and stored at −80°C for 1 week. The platelet counts, platelet distribution width, mean platelet volume (MPV), platelet activity, platelet release, platelet aggregation, platelet metabolism indicators and platelet ultrastructural features (determined by electron microscopy) of the samples at the pre‐freeze, post‐thaw wash (post‐TW) and 24 h post‐thaw wash (24‐PTW) stages were determined and compared.ResultsThe DMSO clearance rate from the post‐TW platelets was 95.56 ± 1.3%, and the platelet recovery rate after washing was 74.66 ± 6.34%. The total count, activity, release factors, aggregation and thrombolytic ability of the post‐TW platelets were lower, whereas the MPV and apoptosis rates were higher compared with those of the pre‐freeze platelets. The lactic acid, glucose and potassium ions released from the platelets during washing were filtered away by the dialyser, which significantly reduced their concentration. However, 24‐PTW platelets were metabolically active, resulting in a decrease in pH and glucose content and an increase in lactic acid content. The level of potassium ions remained low after 24 h of storage and washing. The pre‐freeze platelets maintained their normal disc shape and exhibited an open canalicular system (OCS) and a dense tubular system. The cPLTs appeared irregular after washing, with protruding pseudopodia and an extensive OCS, which increased the release of their contents.ConclusionWe developed a novel dialysis method to effectively remove DMSO from cPLTs under aseptic conditions and maintain platelet quality. The clinical efficacy of our method remains to be determined. However, the function of the platelets declined 24 h after washing, making them unsuitable for transfusion.

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Section: Discussionmentioning

confidence: 99%

Cryopreserved platelets washed with a dialysis machine for dimethyl sulphoxide removal

Huang

Lin

et al. 2023

Vox Sanguinis

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“…ANH is considered as a safe and effective blood conservation technique [ 18 ]. First, unlike preoperative autologous blood donation, it is not needed to collect autologous blood repeatedly and save it for several days before surgery in the ANH group [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

The role of acute normovolemic hemodilution in reducing allogeneic blood transfusion in glioblastoma surgery: a case–control study

Chen,

Zhang,

Wang

et al. 2023

Chin Neurosurg Jl

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Background Acute normovolemic hemodilution (ANH) was first introduced in glioblastoma surgery, and its role in reducing allogeneic blood transfusion was investigated in this study. Methods This study enrolled supratentorial glioblastoma patients who received total resection. In the ANH group, the patients were required to draw blood before the operation, and the blood will be transfused back to the patient during the operation. The association between ANH and clinical features was investigated. Results Sixty supratentorial glioblastoma patients were enrolled in this study, 25 patients were allocated in the ANH group, and another 35 patients were included in the control group. ANH dramatically reduced the need for allogeneic blood transfusion (3 [12%] vs 12 [34.3%], P = 0.049), and the blood transfusion per total of patients was dramatically decreased by the application of ANH (0.40 ± 1.15 units vs 1.06 ± 1.59 units, P = 0.069). Furthermore, ANH also markedly reduced the requirement of fresh frozen plasma (FFP) transfusion (2 [8%] vs 11 [31.4%], P = 0.030) and the volume of FFP transfusion per total of patients (32.00 ± 114.46 mL vs 115.71 ± 181.00 mL, P = 0.033). The complication rate was similar between the two groups. Conclusions ANH was a safe and effective blood conservation technique in glioblastoma surgery.

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“…21 While ANH-collected blood stored at room temperature for up to 8 hours has a hemostatic effect, 22 preserving it at 4 °C helps maintain platelet activity. 23 In our study, we used a 4 °C refrigerator to maximize blood clotting function preservation. Transfusing fresh whole blood can partially enhance coagulation function.…”

Section: Discussionmentioning

confidence: 99%

Safe and Effective Blood Preservation Through Acute Normovolemic Hemodilution and Low-Dose Tranexamic Acid in Open Partial Hepatectomy

Yang,

Zhang,

Luo

et al. 2023

JPR

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In this study, we evaluated the efficacy of tranexamic acid (TXA) and acute normovolemic hemodilution (ANH) with 6% hydroxyethyl starch (130/0.4) in minimizing blood loss during open partial liver resection. Coagulation function was assessed using thromboelastography (TEG) and hemostasis tests, while renal function changes were tracked through serum creatinine values postsurgery. Methods: Thirty patients undergoing open partial liver resection were allocated to two groups: Group T received TXA + ANH, and Group A received ANH alone. Blood was drawn from the radial artery under general anesthesia. Both groups received peripheral vein injections of 6% hydroxyethyl starch 130/0.4. Group T additionally received intravenous TXA. Primary outcomes included blood loss and allogeneic blood transfusions. TEG assessed coagulation status and renal function was monitored. Results: Group T demonstrated superior outcomes compared to Group A. Group T had significantly lower intraoperative blood loss (700 mL vs 1200 mL) and a lower bleeding rate per kilogram of body weight (13.3 mL/kg vs 20.4 mL/kg). Coagulation parameters favored Group T, with higher TEG maximum amplitude (55.91 mm vs 45.88 mm) and lower activated partial thromboplastin time (38.04 seconds vs 41.49 seconds). Neither group experienced acute renal injury or kidney function deficiency during hospitalization. Conclusion: TXA and ANH in a small dose during liver resection stabilize clotting, reduce blood loss by 6% compared to hydroxyethyl starch 130/0.4, and do not affect renal function.

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Platelet function of whole blood after short‐term cold storage: A prospective in vitro observational study

Cited by 4 publications

References 39 publications

Cryopreserved platelets washed with a dialysis machine for dimethyl sulphoxide removal

Cryopreserved platelets washed with a dialysis machine for dimethyl sulphoxide removal

The role of acute normovolemic hemodilution in reducing allogeneic blood transfusion in glioblastoma surgery: a case–control study

Safe and Effective Blood Preservation Through Acute Normovolemic Hemodilution and Low-Dose Tranexamic Acid in Open Partial Hepatectomy

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