Plate‐shape carbonated hydroxyapatite/collagen nanocomposite hydrogel via in situ mineralization of hydroxyapatite concurrent with gelation of collagen at pH = 7.4 and 37°C

Abstract

The objective of this study was to develop a collagen/hydroxyapatite (HA) nanocomposite scaffold for bone tissue engineering applications. For this purpose, in situ mineralization of HA was accompanied with formation of collagen hydrogel at condition similar to the physiological condition, pH = 7.4, and 37 C. The physicochemical and biological properties of the in situ scaffold were compared with nanocomposite fabricated by mixing HA powder and collagen hydrogel (powder-mixed scaffold). The HA in this method was formed in the same condition as the in situ method. X-ray diffraction and FTIR analysis of in situ scaffold showed the formation of carbonated HA, similar to bone, while the HA powder in powder-mixed scaffold showed non-carbonated structure. Scanning electron microscopy revealed the formation of fibrillated collagen in both composites. HA was observed in both scaffolds, but with different morphology. The in situ formed HA had a plate-like morphology while the preformed HA showed spherical morphology in the powder-mixed scaffold. The invitro cytocompatibility and osteogenesis activity of scaffolds using osteoblast-like cells (MG-63) showed higher cytocompatibility and more osteogenesis capability of the in situ scaffold in comparison with the powder-mixed scaffold. The results suggest the in situ method as a proper approach for fabrication of HA/collagen scaffolds with similar properties like bone.

Collagen/chitosan-functionalized graphene oxide hydrogel provide a 3D matrix for neural stem/precursor cells survival, adhesion, infiltration and migration

“…The diffraction pattern of pure Col hydrogel showed a broad peak that indicated an amorphous structure of collagen. 52 The peaks at 2θ values of 31° and 45° in the XRD patterns of Col and Col/CSGO hydrogels might be due to presence of NaCL in hydrogels. 53 As PBS is used for hydrogel preparation, the presence of PBS salts in the final dried hydrogel is possible.…”

Current Insights into Collagen Type I

“…A low level of WVTR will cause the accumulation of exudates while an extreme level of WVTR water evaporates at the injury site, leading to a state of dehydration. An optimum range of WVTR within the range of 2028.3 ± 237.8 g/m 2 /day is essential to maintain a moist environment, as well as to enhance the normal healing phase [ 76 ].…”

Comparison capacity of collagen hydrogel, mix‐powder and in situ hydroxyapatite/collagen hydrogelscaffolds with and without mesenchymal stem cells and platelet‐rich plasma in regeneration of critical sized bone defect in a rabbit animal model

“…Collagen hydrogel, Co/HA hydrogel and in-situ collagen/HA scaffolds were prepared and fabricated as previously described by Takallu et al, (2019). 11 A total of 13.9 mg collagen was dissolved in 1.8 ml acetic acid (0.02 M) for preparation of collagen scaffold. To reach a transparent solution with collagen concentration of 7.72 mg.ml-1 the solution was HA/Co under magnetic stirring.…”

“…10 Incorporation of inorganic materials such as hydroxyapatite in polymeric hydrogels has been shown to be a suitable option to overcome these problems. 11 Hydroxyapatite (Ca10(PO4)6(OH)2) is one of the main inorganic components of bone. It is widely applied in the field of BTE owing to its excellent osteoconductivity, biocompatibility, and biointegration properties.…”

“…14 The in situ method has been recently introduced as a proper approach for fabrication of HA/collagen scaffolds with similar properties like bone. 11 The scaffolds can be seeded with living stem cells and growth factors to support the organization of the damaged tissues. 15 BM-MSCs have been shown to be in the spotlight of cell therapy due to their ability to differentiation of many types of cell lineages.…”