Plasmonic Nanostructures for SERRS Multiplexed Identification of Tumor‐Associated Antigens

Meneghetti, Moreno; Scarsi, Alessia; Litti, Lucio; Marcolongo, Gabriele; Amendola, Vincenzo; Gobbo, Marina; Chio, Marzia Di; Boscaini, Anita; Fracasso, Giulio; Colombatti, Marco

doi:10.1002/smll.201201196

Cited by 36 publications

(67 citation statements)

References 24 publications

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“…In the present case, we exploited the surface chemistry of the alloy nanoparticles to add stealth properties by conjugation with thiolated polyethylene glycol (PEG) . In addition, bright SERS signals require stable anchoring of Raman active dyes in the electromagnetic hot spots at the junction between two plasmonic nanoparticles, hence we also exploited Au‐S bonding for loading nanoalloys with a di‐thiolated sulforhodamine 101 (DTSR) . In our synthetic procedure, the addition of DTSR and PEG to the Au‐Fe alloy dispersion takes place simultaneously, yielding small nanoparticle aggregates with overall size of 30‐60 nm (Figure b and Figure S2 in S.I.).…”

Section: Resultsmentioning

confidence: 99%

“…Less information is available about the biocompatibility of Raman‐active dyes connected to the surface of metal nanoparticles, although no exposure limits are prescribed for these dyes in their material safety data sheet (MSDS), and negligible in vivo toxicity was observed up to 20 mg/Kg and 5 mg/Kg of injected sulforhodamine 101 and Si‐naphthalocyanine, respectively . In general, no evidence of toxicity has been reported in case of Raman‐active dyes conjugated to the surface of Au NPs . The cytotoxicity of our MCAs was assessed with XTT assays on mouse mammary tumor cells (4T1, Figure a) and also human histiocytic lymphoma cells (U937, Figure b).…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, taking advantage of the easy reaction between free thiol groups and Au atoms in our Au‐Fe nanoalloy, several future improvements are possible in these MCAs, for instance to increase their selectivity for diseased tissues by adding targeting functionalities (i.e. antibodies, peptides or DNA strands derivatized with sulfhydryl groups), or by engineering their surface to enhance the ability to penetrate cell membranes …”

Section: Discussionmentioning

confidence: 99%

“…The solution was kept at 60 °C for 1 hour and then the NPs conjugates were purified by several dialysis steps (10 kDa membranes, from Sartorius) and resuspended in deionized water. DTSR was synthesized according to a previously described procedure . For the synthesis of DTNC, we dissolved 49.5 mg of Silicon 2,3‐naphthalocyanine dichloride (NPCyCl 2 , from Sigma) in 3 mL of mercaptoethanol (Sigma) and a drop of triethilamine (TEA).…”

Section: Methodsmentioning

confidence: 99%

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Magneto‐Plasmonic Au‐Fe Alloy Nanoparticles Designed for Multimodal SERS‐MRI‐CT Imaging

Amendola

Scaramuzza

Litti

et al. 2014

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Diagnostic approaches based on multimodal imaging are needed for accurate selection of the therapeutic regimens in several diseases, although the dose of administered contrast drugs must be reduced to minimize side effects. Therefore, large efforts are deployed in the development of multimodal contrast agents (MCAs) that permit the complementary visualization of the same diseased area with different sensitivity and different spatial resolution by applying multiple diagnostic techniques. Ideally, MCAs should also allow imaging of diseased tissues with high spatial resolution during surgical interventions. Here a new system based on multifunctional Au-Fe alloy nanoparticles designed to satisfy the main requirements of an ideal MCA is reported and their biocompatibility and imaging capability are described. The MCAs show easy and versatile surface conjugation with thiolated molecules, magnetic resonance imaging (MRI) and computed X-ray tomography (CT) signals for anatomical and physiological information (i.e., diagnostic and prognostic imaging), large Raman signals amplified by surface enhanced Raman scattering (SERS) for high sensitivity and high resolution intrasurgical imaging, biocompatibility, exploitability for in vivo use and capability of selective accumulation in tumors by enhanced permeability and retention effect. Taken together, these results show that Au-Fe nanoalloys are excellent candidates as multimodal MRI-CT-SERS imaging agents.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Magneto‐Plasmonic Au‐Fe Alloy Nanoparticles Designed for Multimodal SERS‐MRI‐CT Imaging

Amendola

Scaramuzza

Litti

et al. 2014

Small

Self Cite

169

174

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“…By fitting this spectrum one estimates that the average dimension of the AuNPs is 20 nm and their concentration 10 −9 M. The aggregated AuNPs present a characteristic red-shifted plasmon resonance band, according to the Mie-Gans model used for size and concentration evaluation of gold colloidal solution [28]. Aggregates composed by few tens of nanoparticles, 10-20 AuNPs, cause a typical band at 600-800 nm, as just reported before [33,34].…”

Section: Characterisation Of Au Colloidal Solutionsmentioning

confidence: 79%

Use of nano gold obtained by laser ablation for SEIRA analyses of colorants

et al. 2014

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The analysis of dyes in cultural heritage samples is a well-known challenging task, due to their inherent high tinting strength and consequent low concentration in the carrying matrix a fact that severely limits the number of analytical techniques that can be efficiently and micro-destructively employed for their detection and unambiguous identification. In the present study, an advanced and alternative SEIRA based analytical protocol for the analysis of small quantities of synthetic colorants has been proposed. The method has been set up for the identification of Acid Orange 7 (AO7) using Au nanoparticles obtained by laser ablation in solution (LASiS). Analyses have been performed applying a drop containing a mixture between the colorant and the Au colloidal solution in its unaggregated state on a gold coated glass slide for RAS (Reflection Absorption Spectroscopy) analysis. The first results showed that, thanks to the enhancement produced by the nanoparticles, it is possible to analyze small amount of diluted solutions containing the colorant. Thus, the method has been successfully applied for the analysis of few pieces of dyed wool, after the development of a suitable micro extraction procedure.

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Enhanced EGFR Targeting Activity of Plasmonic Nanostructures with Engineered GE11 Peptide

Biscaglia

Rajendran

Conflitti

et al. 2017

Adv Healthcare Materials

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Plasmonic nanostructures show important properties for biotechnological applications, but they have to be guided on the target for exploiting their potentialities. Antibodies are the natural molecules for targeting. However, their possible adverse immunogenic activity and their cost have suggested finding other valid substitutes. Small molecules like peptides can be an alternative source of targeting agents, even if, as single molecules, their binding affinity is usually not very good. GE11 is a small dodecapeptide with specific binding to the epidermal growth factor receptor (EGFR) and low immunogenicity. The present work shows that thousands of polyethylene glycol (PEG) chains modified with lysines and functionalized with GE11 on clusters of naked gold nanoparticles, obtained by laser ablation in water, achieves a better targeting activity than that recorded with nanoparticles decorated with the specific anti-EGFR antibody Cetuximab (C225). The insertion of the cationic spacer between the polymeric part of the ligand and the targeting peptide allows for a proper presentation of GE11 on the surface of the nanosystems. Surface enhanced resonance Raman scattering signals of the plasmonic gold nanoparticles are used for quantifying the targeting activity. Molecular dynamic calculations suggest that subtle differences in the exposition of the peptide on the PEG sea are important for the targeting activity.

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Plasmonic Nanostructures for SERRS Multiplexed Identification of Tumor‐Associated Antigens

Cited by 36 publications

References 24 publications

Magneto‐Plasmonic Au‐Fe Alloy Nanoparticles Designed for Multimodal SERS‐MRI‐CT Imaging

Magneto‐Plasmonic Au‐Fe Alloy Nanoparticles Designed for Multimodal SERS‐MRI‐CT Imaging

Use of nano gold obtained by laser ablation for SEIRA analyses of colorants

Enhanced EGFR Targeting Activity of Plasmonic Nanostructures with Engineered GE11 Peptide

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