Plasmid-mediated colistin resistance encoded by mcr-1 gene in Escherichia coli co-carrying blaCTX-M-15 and blaNDM-1 genes in pediatric patients in Qatar

Tsui, Clement K. M.; Sundararaju, Sathyavathi; Mana, Hassan Al; Hasan, Mohammad Rubayet; Tang, Patrick; Pérez-López, Andrés

doi:10.1016/j.jgar.2020.06.029

Cited by 9 publications

(11 citation statements)

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“…Contrary to the diversity of species and sequence types of MCR expressing isolates encountered, mcr-carrying plasmids identified in this study belonged to only three Inc types, i.e., IncX4, IncI2 and IncHI2. This relative paucity of the plasmid scaffolds compared to the variation of the diversity of clones is in agreement with global observations [2,5], as well as with those from other Middle Eastern countries outside of the Arabian Peninsula [13,14,16,18,20,22]. With the exception of E. coli ST1196 carrying either IncI2 or IncX4 plasmids, the various types of plasmids were associated with specific clones.…”

Section: Discussionsupporting

confidence: 88%

“…Resistance to colistin has been extensively documented in countries of the Arabian Peninsula [8][9][10]. Although mcr-mediated colistin resistance among clinical isolates have been encountered in almost all countries of the region, these were usually sporadic MDR, often carbapenem resistant strains [10][11][12][13][14][15][16][17][18] and the colistin resistance among the majority of them seemed to be chromosomally encoded [9,10]. Contrary of the paucity of mcr harboring clinical isolates, we found that fecal specimens of broiler poultry contained a surprising variety of MCR-producing colistin resistant strains (E. coli of 16 different STs, E. albertii, K. pneumoniae and S. minnesota ), matching or exceeding the results of similar studies from countries of the extended neighborhood [19][20][21][22][23][24].…”

Section: Discussionmentioning

confidence: 99%

“…Carbapenem resistant Enterobacterales (CRE) infections have been a major problem in countries of the Arabian Peninsula [7,8], with colistin resistance rate of >20% reported in certain CRE collections [9,10]. Although various mcr genes have been documented among sporadic human isolates [11][12][13][14][15][16][17][18], other studies did not find them at all [9], suggesting that colistin resistance of clinical isolates in the region is mainly due to chromosomal mutations [10]. While the presence and variety of mcr-carrying strains in poultry has been extensively documented in Egypt, Lebanon and Pakistan [19][20][21][22][23][24], the only similar study in the Arabian Peninsula described 14 mcr-carrying Escherichia coli from Qatar without any further characterization [25].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of MCR-1 Producing Enterobacterales Isolated in Poultry Farms in the United Arab Emirates

et al. 2022

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Data on the prevalence of MCR-producing Enterobacterales of animal origin are scarce from the Arabian Peninsula. We investigated the presence and variety of such strains from fecal specimens of poultry collected in four farms in the United Arab Emirates. Colonies from ten composite samples per farm grown on colistin-supplemented plates were PCR-screened for alleles of the mcr gene. Thirty-nine isolates selected based on species, colony morphology, and plasmid profile were subjected to whole genome sequencing. The panel of their resistance and virulence genes, MLST and cgMLST were established. Transferability and incompatibility types of the MCR-plasmids were determined. mcr-1.1 positive strains were identified in 36 of the 40 samples. Thirty-four multi-drug resistant Escherichia coli of 16 different sequence types, two Escherichia albertii, two Klebsiella pneumoniae and one Salmonella minnesota were identified. Beyond various aminoglycoside, tetracycline, and co-trimoxazole resistance genes, seven of them also carried ESBL genes and one blaCMY-2. Six IncHI2, 26 IncI2 and 4 IncX4 MCR-plasmids were mobilized, in case of the IncHI2 plasmids co-transferring ampicillin, chloramphenicol and tetracycline resistance. The diversity of mcr-1 positive strains suggest a complex local epidemiology calling for a coordinated surveillance including animals, retail meat and clinical cases.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of MCR-1 Producing Enterobacterales Isolated in Poultry Farms in the United Arab Emirates

et al. 2022

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“…Finally, the detection of mcr-1 genes in two E. coli isolates resistant to colistin, the last resort for the treatment of Enterobacterales resistant to broad-spectrum cephalosporins and carbapenems, was worrying but not surprising as this gene has been sporadically reported in clinical specimens in the Arabian Peninsula carried by IncI2 and IncHI2 plasmids (Dandachi et al, 2019). It is worth noting that these isolates belonged to the STs ST115 and ST540, which have mainly been detected from the poultry and environment (Tsui et al, 2020). As fecal carriage of ESBL-producing E. coli has also been described from food handlers in Qatar (Eltai et al, 2018b), our findings suggest that a silent transmission from animals to humans via the food chain may be also playing a role in the spread of ESBL and other resistance determinants among our population.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Extended-Spectrum β-Lactamase–Producing Escherichia coli and Klebsiella pneumoniae Among the Pediatric Population in Qatar

et al. 2020

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Introduction Although extended-spectrum β-lactamase (ESBL)–producing Enterobacterales are a public health problem in the Arabian Peninsula, data on the molecular characteristic of their antimicrobial resistance determinants in children is limited. Aim To determine the molecular characteristics of ESBL-producing Escherichia coli and Klebsiella pneumoniae in the pediatric population of Qatar. Methods Whole-genome sequencing was performed on ESBL-producing E. coli and K. pneumoniae isolates recovered from screening and clinical specimens from pediatric patients at Sidra Medicine in Doha from January to December 2018. Results A total of 327 ESBL producers were sequenced: 254 E. coli and 73 K. pneumoniae . Non-susceptibility rates to non-β-lactam antibiotics for both species were 18.1 and 30.1% for gentamicin, 0.8 and 4.1% for amikacin, 41.3 and 41.1% for ciprofloxacin, and 65.8 and 76.1% for cotrimoxazole. The most common sequence types (STs) were ST131 (16.9%), ST38 and ST10 (8.2% each) in E. coli and ST307 (9.7%), and ST45 and ST268 (6.9% each) in K. pneumoniae . CTX-M type ESBLs were found in all but one isolate, with CTX-M-15 accounting for 87.8%. Among other β-lactamases, TEM-1B and OXA-1 were coproduced in 41 and 19.6% of isolates. The most common plasmid-mediated quinolone resistance genes cocarried were qnr A/B/E/S (45.3%). Ninety percent of gentamicin non-susceptible isolates harbored genes encoding AAC(3) enzymes, mainly aac(3)-IIa . Only two of 57 isolates harboring aac(6′)-Ib-cr were non-susceptible to amikacin. Chromosomal mutations in genes encoding DNA gyrase and topoisomerase IV enzymes were detected in 96.2% fluoroquinolone-non-susceptible E. coli and 26.7% fluoroquinolone-non-susceptible K. pneumoniae . Conclusion Our data show that CTX-M enzymes are largely the most prevalent ESBLs in children in Qatar with a predominance of CTX-M-15. Carbapenem-sparing options to treat ESBL infections are limited, given the frequent coproduction of OXA-1 and TEM-1B enzymes and coresistance to antibiotic classes other than β-lactams.

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“…We detected high abundances of the mcr-1 , bla CMY-2 and bla CTX-M genes in untreated wastewater, which is worrying since they confer resistance to HPCIA in humans 20 . Their potential incorporation into clinically relevant bacteria might narrow antimicrobial treatment options and compromise their efficacy in cases of infection, especially if resistance against different last resort antibiotics (e.g., carbapenemases, mcr ) occurs 34 , 35 . The human impact of livestock as a reservoir for combinations of antimicrobials of the last resort (i.e.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-resistant bacteria, antibiotic resistance genes, and antibiotic residues in wastewater from a poultry slaughterhouse after conventional and advanced treatments

Savin

Alexander

Bierbaum

et al. 2021

Sci Rep

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Slaughterhouse wastewater is considered a reservoir for antibiotic-resistant bacteria and antibiotic residues, which are not sufficiently removed by conventional treatment processes. This study focuses on the occurrence of ESKAPE bacteria (Enterococcus spp., S. aureus, K. pneumoniae, A. baumannii, P. aeruginosa, Enterobacter spp.), ESBL (extended-spectrum β-lactamase)-producing E. coli, antibiotic resistance genes (ARGs) and antibiotic residues in wastewater from a poultry slaughterhouse. The efficacy of conventional and advanced treatments (i.e., ozonation) of the in-house wastewater treatment plant regarding their removal was also evaluated. Target culturable bacteria were detected only in the influent and effluent after conventional treatment. High abundances of genes (e.g., blaTEM, blaCTX-M-15, blaCTX-M-32, blaOXA-48, blaCMY and mcr-1) of up to 1.48 × 106 copies/100 mL were detected in raw influent. All of them were already significantly reduced by 1–4.2 log units after conventional treatment. Following ozonation, mcr-1 and blaCTX-M-32 were further reduced below the limit of detection. Antibiotic residues were detected in 55.6% (n = 10/18) of the wastewater samples. Despite the significant reduction through conventional and advanced treatments, effluents still exhibited high concentrations of some ARGs (e.g., sul1, ermB and blaOXA-48), ranging from 1.75 × 102 to 3.44 × 103 copies/100 mL. Thus, a combination of oxidative, adsorptive and membrane-based technologies should be considered.

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Plasmid-mediated colistin resistance encoded by mcr-1 gene in Escherichia coli co-carrying blaCTX-M-15 and blaNDM-1 genes in pediatric patients in Qatar

Cited by 9 publications

References 5 publications

Molecular Characterization of MCR-1 Producing Enterobacterales Isolated in Poultry Farms in the United Arab Emirates

Molecular Characterization of MCR-1 Producing Enterobacterales Isolated in Poultry Farms in the United Arab Emirates

Molecular Characterization of Extended-Spectrum β-Lactamase–Producing Escherichia coli and Klebsiella pneumoniae Among the Pediatric Population in Qatar

Antibiotic-resistant bacteria, antibiotic resistance genes, and antibiotic residues in wastewater from a poultry slaughterhouse after conventional and advanced treatments

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