volume 32, issue 4, P1072-1077 1993
DOI: 10.1021/bi00055a012
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Ken J. Hampel, Gary D. Burkholder, Jeremy S. Lee

Abstract: The ability of independent pyr.pur tracts to participate in triplex formation has been investigated in linear plasmids. The pyr.pur tract could be positioned at the ends of the plasmids or internally by a suitable choice of restriction enzyme. Dimer formation between plasmids was monitored by mobility shifts on agarose gels as well as by direct visualization in the electron microscope. Linear dimers and X and Y structures were observed. Control experiments showed that a pyr.pur tract was essential and was cons…

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