Plasmid-borne armA methylase gene, together with bla
            CTX-M-15 and bla
            TEM-1, in a Klebsiella oxytoca isolate from China

Zhang, Ying; Zhou, Hua; Shen, Ping; Yu, Yunsong; Li, Lanjuan

doi:10.1099/jmm.0.2008/001271-0

Cited by 20 publications

(10 citation statements)

References 16 publications

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“…FJ715937), pMUR050 of Escherichia coli (AY522431), pCOP1 of E. coli (FJ187822), pCTX-M3 of Citrobacter freundii (AF550415), and pKSMA0710 of Serratia marcescens (FJ917355). The armA ge-netic environment of MDR-ZJ06 is also identical to those of Klebsiella oxytoca and K. pneumoniae, which were isolated from the same region as MDR-ZJ06 (15,33).…”

Section: Resultsmentioning

confidence: 69%

Genomic Analysis of the Multidrug-Resistant Acinetobacter baumannii Strain MDR-ZJ06 Widely Spread in China

Zhou

Zhang

et al. 2011

Antimicrob Agents Chemother

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107

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We previously reported that the multidrug-resistant (MDR) Acinetobacter baumannii strain MDR-ZJ06, belonging to European clone II, was widely spread in China. In this study, we report the whole-genome sequence of this clinically important strain. A 38.6-kb AbaR-type genomic resistance island (AbaR22) was identified in MDR-ZJ06. AbaR22 has a structure similar to those of the resistance islands found in A. baumannii strains AYE and AB0057, but it contained only a few antibiotic resistance genes. The region of resistant gene accumulation as previously described was not found in AbaR22. In the chromosome of the strain MDR-ZJ06, we identified the gene bla oxa-23 in a composite transposon (Tn2009). Tn2009 shared the backbone with other A. baumannii transponsons that harbor bla oxa-23 , but it was bracketed by two ISAba1 elements which were transcribed in the same orientation. MDR-ZJ06 also expressed the armA gene on its plasmid pZJ06, and this gene has the same genetic environment as the armA gene of the Enterobacteriaceae. These results suggest variability of resistance acquisition even in closely related A. baumannii strains.

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Section: Resultsmentioning

confidence: 69%

Genomic Analysis of the Multidrug-Resistant Acinetobacter baumannii Strain MDR-ZJ06 Widely Spread in China

Zhou

Zhang

et al. 2011

Antimicrob Agents Chemother

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107

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“…In different studies, the phenotypic and genotypic resistance of Enterococcus and Salmonella isolates against aminoglycosides has been reported from Iran and all over the world (10)(11)(12)(13)(14)(15)(16)(17). This study aimed to investigate the occurrence of aminoglycoside resistance and the prevalence of the resistance-modifying enzyme genes, acc(3)-Ia, aac(6′)-Ib, acc(3)-IIa, armA and rat in Enterococcus and Salmonella strains isolated from two hospitals in Iran (Imam Khomeni , Ilam and Milad, Tehran, Respectively).…”

Section: Objectivesmentioning

confidence: 99%

Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

Samadi

Pakzad

Sefidan

et al. 2015

Jundishapur J Microbiol

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Background: Aminoglycosides are a group of antibiotics that have been widely used in the treatment of life-threatening infections of Gram-negative bacteria. Objectives: This study aimed to evaluate the frequency of aminoglycoside resistance genes in Enterococcus and Salmonella strains isolated from clinical samples by PCR. Materials and Methods: In this study, 140 and 79 isolates of Enterococcus and Salmonella were collected, respectively. After phenotypic biochemical confirmation, 117 and 77 isolates were identified as Enterococcus and Salmonella, respectively. After the biochemical identification of the isolates, antibiotic susceptibility for screening of resistance was done using the Kirby-Bauer method for gentamicin, amikacin, kanamycin, tobramycin and netilmycin. DNA was extracted from resistant strains and the presence of acc (3)-Ia, aac (3′)-Ib, acc (6)-IIa ,16SrRNA methylase genes (armA and rat) was detected by PCR amplification using special primers and positive controls. Results: Enterococcus isolates have the highest prevalence of resistance to both kanamycin and amikacin (68.4%), and Salmonella isolates have the highest prevalence of resistance against kanamycin (6.9%). Ninety-three and 26 isolates of Enterococcus and Salmonella at least were resistant against one of the aminoglycosides, respectively. Moreover, 72.04%, 66.7%, and 36.6% of the resistant strains of Enterococcus had the aac (3′)-Ia, aac (3′)-IIa, and acc (6′)-Ib genes, respectively. None of the Salmonella isolates have the studied aminoglycoside genes. Conclusions: Our results indicate that acetylation genes have an important role in aminoglycoside resistance of the Enterococcus isolates from clinical samples. Moreover, Salmonella strains indicate very low level of aminoglycoside resistance, and aminoglycoside resistance genes were not found in Salmonella isolates. These results indicate that other resistance mechanisms, including efflux pumps have an important role in aminoglycoside resistance of Salmonella.

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“…The isolate with the highest number of acquired resistance genes (n ϭ 16) was the NDM-1-producing K. oxytoca isolate, which had several genes encoding resistance to ␤-lactams, aminoglycosides, sulfonamides, quinolones, chloramphenicol, macrolides, rifampin, tetracycline, and trimethoprim; in addition, the genome of this isolate encoded the methyltransferase gene armA, which is of special relevance since it confers high levels of resistance to all clinically important aminoglycosides (14). ArmA has been associated with K. oxytoca in Japan and China (15,16); however, ArmA has only sporadically been recovered from Enterobacteriaceae isolates in Spain, and these were mainly obtained from animals (17,18), but it has not been recovered from K. oxytoca. The association of the bla NDM-1 and armA genes in the same plasmid has been described previously (19).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Carbapenemase-Producing Klebsiella oxytoca in Spain, 2016–2017

Pérez-Vázquez

Oteo

Sola‐Campoy

et al. 2019

Antimicrob Agents Chemother

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There is little information about carbapenemase-producing (CP) Klebsiella oxytoca, an important nosocomial pathogen. We characterized CP K. oxytoca isolates collected from different Spanish hospitals between January 2016 and October 2017. During the study period, 139 nonduplicate CP K. oxytoca isolates were identified; of these, 80 were studied in detail. Carbapenemase and extendedspectrum ␤-lactamase genes were identified by PCR and sequencing. Genetic relatedness was studied by pulsed-field gel electrophoresis (PFGE). Whole-genome sequencing (WGS), carried out on 12 representative isolates, was used to identify the resistome, to elucidate the phylogeny, and to determine the plasmids harboring carbapenemase genes. Forty-eight (60%) isolates produced VIM-1, 30 (37.5%) produced OXA-48, 3 (3.7%) produced KPC-2, 2 (2.5%) produced KPC-3, and 1 (1.2%) produced NDM-1; 4 isolates coproduced two carbapenemases. By PFGE, 69 patterns were obtained from the 80 CP K. oxytoca isolates, and four well-defined clusters were detected: cluster 1 consisted of 11 OXA-48-producing isolates, and the other three clusters included VIM-1-producing isolates (5, 3, and 3 isolates, respectively). In the 12 sequenced isolates, the average number of acquired resistance genes was significantly higher in VIM-1-producing isolates (10.8) than in OXA-48-producing isolates (2.3). All 12 isolates had chromosomally encoded genes of the bla OXY-2 genotype, and by multilocus sequence typing, most belonged to sequence type 2 (ST2). Carbapenemase genes were carried by IncL, IncHI2, IncFII, IncN, IncC, and IncP6 plasmid types. The emergence of CP K. oxytoca was principally due to the spread of VIM-1-and OXA-48-producing isolates in which VIM-1-and OXA-48 were carried by IncL, IncHI2, IncFII, and IncN plasmids. ST2 and the genotype bla OXY-2 predominated among the 12 sequenced isolates.

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Plasmid-borne armA methylase gene, together with bla CTX-M-15 and bla TEM-1, in a Klebsiella oxytoca isolate from China

Cited by 20 publications

References 16 publications

Genomic Analysis of the Multidrug-Resistant Acinetobacter baumannii Strain MDR-ZJ06 Widely Spread in China

Genomic Analysis of the Multidrug-Resistant Acinetobacter baumannii Strain MDR-ZJ06 Widely Spread in China

Study of Aminoglycoside Resistance Genes in Enterococcus and Salmonella Strains Isolated From Ilam and Milad Hospitals, Iran

Characterization of Carbapenemase-Producing Klebsiella oxytoca in Spain, 2016–2017

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