Plasma-treated medium tunes the inflammatory profile in murine bone marrow-derived macrophages

Bekeschus, Sander; Scherwietes, Lukas; Freund, Eric; Liedtke, Kim Rouven; Hackbarth, Christine; Woedtke, Thomas von; Partecke, Lars-Ivo

doi:10.1016/j.cpme.2018.06.001

Cited by 16 publications

(16 citation statements)

References 84 publications

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“…Plasma-treated cell line-derived macrophages were shown to have a higher migratory activity [114], cytokine release [115], and augmented antitumor toxicity [116], which contributed to elevated levels of TNFα [117] when investigated in transwell co-culture systems. Moreover, plasma treatment was suggested to modulate the differentiation patterns of primary murine [118] and human monocyte-derived macrophages [119]. Using human cell line-derived macrophages, this change in differentiation was also attributed to enhanced antitumor effects in direct co-culture experiments [102].…”

Section: Induction Of An Immune Response Through Cap Treatmentmentioning

confidence: 99%

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Semmler

Bekeschus

Schäfer

et al. 2020

Cancers

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149

127

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Recently, the potential use of cold atmospheric pressure plasma (CAP) in cancer treatment has gained increasing interest. Especially the enhanced selective killing of tumor cells compared to normal cells has prompted researchers to elucidate the molecular mechanisms for the efficacy of CAP in cancer treatment. This review summarizes the current understanding of how CAP triggers intracellular pathways that induce growth inhibition or cell death. We discuss what factors may contribute to the potential selectivity of CAP towards cancer cells compared to their non-malignant counterparts. Furthermore, the potential of CAP to trigger an immune response is briefly discussed. Finally, this overview demonstrates how these concepts bear first fruits in clinical applications applying CAP treatment in head and neck squamous cell cancer as well as actinic keratosis. Although significant progress towards understanding the underlying mechanisms regarding the efficacy of CAP in cancer treatment has been made, much still needs to be done with respect to different treatment conditions and comparison of malignant and non-malignant cells of the same cell type and same donor. Furthermore, clinical pilot studies and the assessment of systemic effects will be of tremendous importance towards bringing this innovative technology into clinical practice.

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Section: Induction Of An Immune Response Through Cap Treatmentmentioning

confidence: 99%

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Semmler

Bekeschus

Schäfer

et al. 2020

Cancers

Self Cite

149

127

View full text Add to dashboard Cite

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“…Several papers have focused on the plasma treatment of macrophagic populations [21][22][23][24][25]. In particular, the direct or indirect plasma treatment of macrophages can increase migration, an important immune cell function against diseases, and anti-tumor function [26,27].…”

Section: Introductionmentioning

confidence: 99%

Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness

Crestale

Laurita

Liguori

et al. 2018

Plasma

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Monocytes are involved in innate immune surveillance, establishment and resolution on inflammation, and can polarize versus M1 (pro-inflammatory) or M2 (anti-inflammatory) macrophages. The possibility to control and drive immune cells activity through plasma stimulation is therefore attractive. We focused on the effects induced by cold-atmospheric plasma on human primary monocytes and monocyte-derived macrophages. Monocytes resulted more susceptible than monocyte-derived macrophages to the plasma treatment as demonstrated by the increase in reactive oxygen (ROS) production and reduction of viability. Macrophages instead were not induced to produce ROS and presented a stable viability. Analysis of macrophage markers demonstrated a time-dependent decrease of the M1 population and a correspondent increase of M2 monocyte-derived macrophages (MDM). These findings suggest that plasma treatment may drive macrophage polarization towards an anti-inflammatory phenotype.

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“…In vitro, promising studies with gas plasma-oxidized liquids for immunomodulation have been published. An altered differentiation profile of bone marrow-derived macrophages was observed [122]. Changes in the tumor cells' phenotype, accompanied by upregulation of CRT, as well as heat shock proteins and HMGB1, were identified, while immunosuppressive markers such as PD-L1 (crucial for T-cell function) on tumor cells were found to be downregulated [65,67].…”

Section: Mechabisms Of Action and Selection Of Suitable Liquidsmentioning

confidence: 98%

Gas Plasma-Oxidized Liquids for Cancer Treatment: Preclinical Relevance, Immuno-Oncology, and Clinical Obstacles

Freund

Bekeschus

2021

IEEE Trans. Radiat. Plasma Med. Sci.

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Gas plasmas, often referred to as cold physical plasma, are currently being investigated for their potential to serve as anti-cancer agents. Along similar lines, gas plasma-oxidized liquids as a carrier for reactive oxygen species have found their way into pre-clinical research. This review focuses on in vivo studies that utilized such gas plasma-oxidized liquids for cancer therapies. These pre-clinical tumor models, treatment modalities, and types of liquids that were used are summarized and critically discussed. Among these studies, significant results were observed, indicating the potential of oxidative liquids to serve as an anticancer treatment. However, several steps have to be taken to enhance the quality and translational capacities of this approach in order to gain clinical acceptance for possible future cancer therapies. The most crucial steps include not only a careful selection of suitable liquids, with respect to their approval as medical products, but also the consideration of orthotopic and immunocompetent animal tumor models. This would increase the relevance of such studies and simultaneously allow studying the contribution of the most potent of all anti-cancer effectors, the immune system.

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Plasma-treated medium tunes the inflammatory profile in murine bone marrow-derived macrophages

Cited by 16 publications

References 84 publications

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment

Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness

Gas Plasma-Oxidized Liquids for Cancer Treatment: Preclinical Relevance, Immuno-Oncology, and Clinical Obstacles

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