Plasma Polysaccharide Fraction Containing Uronic Acid, in Normal Subjects and in Patients With Rheumatoid Arthritis 1

The glycosaminoglycans of human plasma

“…The average values of total GAG for normal adults are in good agreement with those reported previously by other authors (2,4,6). In view of the close agreement between duplicate measurements, the great variability found when repetitive analyses were performed on the same subject on different days should not be considered as due to limitations of the proposed technique, but rather as a possible indication that plasma GAG reflect fairly active metabolic processes taking place at the tissue level.…”

“…In the method of Badin et al (2), tryptic digestion of the "euglobulin" fraction was emphasized as a preliminary step to the precipitation of GAG. In the method of Bollet, however, the "euglobulin" fraction was treated for 30 min with 0.4 M perchloric acid at room t0, a treatment that could hardly break covalent bonds between protein and GAG.…”

Acid Mucopolysaccharides in Normal Serum1

“…The method described for the quantitative determination of uronic acid containing mucopolysaccharide in serum is essentially a modification of the method of Badin, Schubert, and Vouras (1). Considerably less starting material is used, and digestion of euglobulin precipitate for three days with pepsin and then three days with trypsin is omitted, since it was found that perchloric acid removed a sufficient proportion of protein from the euglobulin solution without loss of uronatecontaining polysaccharide.…”

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Mucopolysaccharide which contains uronic acid has recently been described in normal plasma (1,2). A method for the quantitation of plasma acid mucopolysaccharide was described by Badin, Schubert and Vouras (1), based on the observation that chondroitin sulfate added to plasma is found in euglobulin precipitates (3).

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“…A method for the quantitation of plasma acid mucopolysaccharide was described by Badin, Schubert and Vouras (1), based on the observation that chondroitin sulfate added to plasma is found in euglobulin precipitates (3). These authors reported an increased amount of acid mucopolysaccharide in the plasma of 10 patients with rheumatoid arthritis (1).…”