Plasma membrane protein sorting in polarized epithelial cells.

Mostov, Keith E.; Apodaca, Gerard; Aroeti, Benjamin; Okamoto, Curtis T.

doi:10.1083/jcb.116.3.577

Cited by 263 publications

(183 citation statements)

References 69 publications

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“…The kidney-derived epithelial cell line, MDCK, is polarized in culture and has been extensively used to study the mechanisms and pathways underlying protein sorting in polarized epithelial cells and signals that target proteins to the apical and basolateral cell surfaces have been identified (Rodriguez-Boulan and Nelson, 1989;Mostov et al, 1992). We have previously demonstrated that an endogenous MDCK LAMP-2 is targeted in a polar fashion to the basolateral early endosome/plasma membrane before delivery to the lysosome (Nabi et al, 1991), a route similar to that described for the biosynthetic pathway of LEP100 (avian LAMP-1) in nonpolarized cells (Mathews et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Increased LAMP-2 polylactosamine glycosylation is associated with its slower Golgi transit during establishment of a polarized MDCK epithelial monolayer.

Nabi

Rodríguez-Boulan

1993

MBoC

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An endogenous Madin-Darby canine kidney (MDCK) lysosomal membrane glycoprotein that exhibits a basolateral targeting pathway to the lysosome is shown here to exhibit significant N-terminal amino acid sequence identity to lysosomal associated membrane proteins (LAMP-2) of other species. During establishment of the MDCK monolayer after only 1 d of culture, this canine LAMP-2 has a larger molecular size (110 kDa) than following formation of a confluent monolayer after 3 d of culture (100 kDa) due to the increased presence of N-linked polylactosamine oligosaccharide chains. Neither polylactosamine glycosylation of LAMP-2 in MDCK cells nor truncation of N-linked oligosaccharide chains of LAMP-2 in a ricin-resistant MDCK-RCAR cell line influenced the basolateral polarity of its targeting. However, the rate of basolateral delivery of LAMP-2 in MDCK cells plated for 3 d was significantly faster (tl/2 = 28 min) than in 1-d cells (tl/2 = 40 min); in MDCK-RCAR cells the rate of basolateral delivery at both 1 and 3 d of plating was similar (t1/2 = 40 min). The rate differential in MDCK cells occurred after arrival of LAMP-2 to the Golgi apparatus because the rate of acquisition of endoglycosidase H resistance was the same (t1/2 = 25 min) at both days of plating. The rate of transit of LAMP-2 through the Golgi apparatus to the basolateral domain was therefore far more rapid (-4-fold) in 3 d compared with 1-d MDCK cultures. The increased polylactosamine glycosylation of MDCK LAMP-2 at early times of plating during the establishment of a confluent epithelial monolayer may thus be related to its longer residence time in the Golgi apparatus.

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Section: Discussionmentioning

confidence: 99%

Increased LAMP-2 polylactosamine glycosylation is associated with its slower Golgi transit during establishment of a polarized MDCK epithelial monolayer.

Nabi

Rodríguez-Boulan

1993

MBoC

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“…ARF is also involved in assembly of the nuclear envelope (Boman et al, 1992 Bomsel and Mostov, 1991;Mostov et al, 1992). These cells must sort plasma membrane proteins to the correct destination.…”

Section: Other Possible Functions For Arfmentioning

confidence: 99%

Role of heterotrimeric G proteins in membrane traffic.

Bomsel

Mostov

1992

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“…Transcytosis is an important membrane trafficking process in polarized cells because it allows the exchange of molecules from one cell surface to the other in either direction (7,8). One example of apical to basolateral transcytosis is the Fc receptor (FcRn), which is delivered to the apical membrane of enterocytes and is then transcytosed to the basolateral domain.…”

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confidence: 99%

“…Transcytosis is also central to the establishment of cell polarity and may thus be fundamental in ontogeny and evolution (8,11).…”

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Detergent Insoluble Microdomains are not Involved in Transcytosis of Polymeric Ig Receptor in FRT and MDCK Cells

Sarnataro

Nitsch

Hunziker

et al. 2000

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In polarized epithelial cells, sorting of proteins and lipids to the apical or basolateral domain of the plasma membrane can occur via direct or indirect (transcytotic) pathways from the trans Golgi network (TGN). The 'rafts' hypothesis postulates that the key event for direct apical sorting of some transmembrane proteins and the majority of GPI-anchored proteins depends on their association with glycosphingolipid and cholesterol enriched microdomains (rafts). However, the mechanism of indirect sorting to the apical membrane is not clear. The polyimmunoglobulin receptor (pIgR) is one of the best studied proteins that follow the transcytotic pathway. It is normally delivered from the TGN to the basolateral surface of polarized Madin-Darby Canine Kidney (MDCK) cells from where it transports dIgA or dIgM to the apical surface. We have studied the intracellular trafficking of pIgR in Fischer rat thyroid cells (FRT), and have investigated the sorting machinery involved in transcytosis of this receptor in both FRT and MDCK cells. We found that, in contrast with MDCK cells, a significant amount ( 30%) of pIgR reaches the apical surface by a direct pathway. Furthermore, in both cell lines it does not associate with Triton X-100 insoluble microdomains, suggesting that at least in these cells 'rafts' are not involved in basolateral to apical transcytosis.

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Plasma membrane protein sorting in polarized epithelial cells.

Cited by 263 publications

References 69 publications

Increased LAMP-2 polylactosamine glycosylation is associated with its slower Golgi transit during establishment of a polarized MDCK epithelial monolayer.

Increased LAMP-2 polylactosamine glycosylation is associated with its slower Golgi transit during establishment of a polarized MDCK epithelial monolayer.

Role of heterotrimeric G proteins in membrane traffic.

Detergent Insoluble Microdomains are not Involved in Transcytosis of Polymeric Ig Receptor in FRT and MDCK Cells

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