Plasma membrane N-glycoproteome analysis of wheat seedling leaves under drought stress

Chang, Yanan; Zhu, Dong; Duan, Wenjing; Deng, Xiong; Zhang, Junwei; Ye, Xingguo; Yan, Yueming

doi:10.1016/j.ijbiomac.2021.10.217

Cited by 8 publications

(5 citation statements)

References 64 publications

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“…The N -glycoproteomic analysis of the plasma membrane in wheat under drought stress was performed using HILIC enrichment and LC-MS/MS. N -glycosylation sites were concentrated within the [N × T] motif, and 79.5% of them were located on the random coil, which facilitated protein glycosylation and enhanced structural stability during drought stress [ 65 ]. The role of protein glycosylation in strengthening the cell wall and remodeling the plasma membrane is evident during abiotic stresses such as drought.…”

Section: Crop Proteomics Of Post-translational Modificationsmentioning

confidence: 99%

Crop Proteomics under Abiotic Stress: From Data to Insights

Kausar

Wang

Komatsu

2022

Plants

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Food security is a major challenge in the present world due to erratic weather and climatic changes. Environmental stress negatively affects plant growth and development which leads to reduced crop yields. Technological advancements have caused remarkable improvements in crop-breeding programs. Proteins have an indispensable role in developing stress resilience and tolerance in crops. Genomic and biotechnological advancements have made the process of crop improvement more accurate and targeted. Proteomic studies provide the information required for such targeted approaches. The crosstalk among cellular components is being analyzed by subcellular proteomics. Additionally, the functional diversity of proteins is being unraveled by post-translational modifications during abiotic stress. The exploration of precise cellular responses and the networking among different cellular organelles help in the prediction of signaling pathways and protein–protein interactions. High-throughput mass-spectrometry-based protein studies are now possible due to incremental advancements in mass-spectrometry techniques, sample protocols, and bioinformatic tools as well as the increasing availability of plant genome sequence information for multiple species. In this review, the key role of proteomic analysis in identifying the abiotic-stress-responsive mechanisms in various crops was summarized. The development and availability of advanced computational tools were discussed in detail. The highly variable protein responses among different crops have provided a wide avenue for molecular-marker-assisted genetic buildup studies to develop smart, high-yielding, and stress-tolerant varieties to cope with food-security challenges.

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Section: Crop Proteomics Of Post-translational Modificationsmentioning

confidence: 99%

Crop Proteomics under Abiotic Stress: From Data to Insights

Kausar

Wang

Komatsu

2022

Plants

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“…After tryptic digestion, the labeling of the peptides can be performed by grafting different chemical tags onto their amine groups to obtain quantitative data, allowing the comparison of different experimental conditions, e.g., using the Tandem Mass Tagging (TMT) [ 33 ] or the Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) [ 34 ] technologies.…”

Section: An Overview Of the Strategiesmentioning

confidence: 99%

“…N -glycans can be removed from glycopeptides by an enzymatic digestion with endo-glycosidase H (endo H, from Streptomyces plicatus ) [ 26 ], peptide: N -glycosidase (PNGase) A (from almonds) [ 32 , 34 , 35 , 36 ], or PNGase F (from Flavobacterium meningosepticum ) [ 26 , 33 , 34 , 36 , 37 , 38 ] ( Figure 4 ). Endo-H cleaves the chitobiose core of high mannose and some hybrid N -glycans.…”

Section: An Overview Of the Strategiesmentioning

confidence: 99%

“…Several plants have been studied: Marchantia polymorpha [ 52 ] as an early divergent plant; Zea mays [ 34 ], Triticum aestivum [ 33 ] and Brachypodium distachyon [ 53 ] as monocot plants; and Fagopyrum tataricum [ 38 ], Solanum lycopersicum [ 32 , 37 , 54 ], Gossypium hirsutum [ 55 ], Camellia sinensis [ 56 ], Arabidopsis thaliana [ 22 , 24 , 36 , 43 , 57 , 58 ] and Brassica oleracea [ 23 ] as dicot plants. N -glycoproteomes have been described in different organs: whole thalli corresponding to the haploid gametophytic stage of M. polymorpha [ 52 ]; seedlings [ 59 ]; aerial organs for flowering plants, such as actively growing hypocotyls of etiolated seedlings [ 24 ]; leaves [ 33 , 34 , 43 , 56 ]; stems [ 22 , 43 ]; inflorescences [ 58 ]; fruit pericarp [ 32 , 37 , 54 ]; and seeds [ 38 , 55 ]. The N -glycoproteome of the xylem sap of B. oleracea has also been analyzed, and looks very similar to a cell wall proteome [ 23 ].…”

Section: An Overview Of the Present N -Glycoproteo...mentioning

confidence: 99%

“…N -glycosylation can be regulated at different levels. In T. aestivum seedling leaves, the four following motifs were found to be present in plasma membrane-associated proteins and their frequency was found to be modified upon drought stress: Asn-X-Thr (51.2%), Asn-X-Ser (40.0%), Asn-X-Thr-Ala (4.6%), and Ala-Asn-X-Thr (4.2%) with X≠Pro [ 33 ]. In Z. mays seedling leaves, two motifs were found: Asn-X-Thr (58%) and Asn-X-Ser (42%) [ 34 ].…”

Section: The Heterogeneity Of Plant N -Glycansmentioning

confidence: 99%

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N-glycoproteins in Plant Cell Walls: A Survey

Clemente

Jamet

2022

Plants

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Cell walls are an extracellular compartment specific to plant cells, which are not found in animal cells. Their composition varies between cell types, plant species, and physiological states. They are composed of a great diversity of polymers, i.e., polysaccharides, proteins, and lignins. Cell wall proteins (CWPs) are major players involved in the plasticity of cell walls which support cell growth and differentiation, as well as adaptation to environmental changes. In order to reach the extracellular space, CWPs are transported through the secretory pathway where they may undergo post-translational modifications, including N-glycosylations on the Asn residues in specific motifs (Asn-X-Ser/Thr-X, with X≠Pro). This review aims at providing a survey of the present knowledge related to cell wall N-glycoproteins with (i) an overview of the experimental workflows, (ii) a selection of relevant articles dedicated to N-glycoproteomics, (iii) a description of the diversity of N-glycans, and (iv) a focus on the importance of N-glycans for CWP structure and/or function.

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Candidate genes controlling alkaline-saline tolerance in two different growing stages of wheat life cycle

Mourad,

Farghly,

Börner

et al. 2023

Plant Soil

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Background Alkaline-saline (AS) stress threats crop development and productivity. Understanding the genetic control of AS tolerance in wheat is important to produce wheat cultivars that outstand such a severe stress condition. Methods A set of 48 cultivars were tested under controlled and AS stress conditions at seedling and maturity stages. The effect of AS on seedlings and kernel traits was measured to select tolerant and high-yielding genotypes. Single-marker-analysis (SMA) and gene enrichment were conducted to understand the genetic control of AS tolerance in both growth stages. Results AS stress decreased all kernel traits and most of the seedling traits. High correlations were found between the studied traits in each growth stage. The correlation between the traits related to both stages was non-significant. SMA identified a total of 292 and 52 markers significantly associated with the studied traits under controlled and AS stress conditions. Seven and 20 gene models were identified to control AS tolerance in each stage. Gene enrichment analysis identified one and six networks that control AS tolerance. Four genotypes were selected as superior genotypes. Conclusion The genetic control of the studied traits differs under control and AS conditions. Two genetic systems control AS tolerance in each growth stage. This study is the first one that unlocked the genetic control of AS tolerance in seedling and mature growth stages and identified the biological process that lead to this tolerance. Four genotypes were selected for crossing in future breeding programs to improve AS tolerance in spring wheat.

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Plasma membrane N-glycoproteome analysis of wheat seedling leaves under drought stress

Cited by 8 publications

References 64 publications

Crop Proteomics under Abiotic Stress: From Data to Insights

Crop Proteomics under Abiotic Stress: From Data to Insights

N-glycoproteins in Plant Cell Walls: A Survey

Candidate genes controlling alkaline-saline tolerance in two different growing stages of wheat life cycle

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