Plasma lipid levels and risk of primary open angle glaucoma: a genetic study using Mendelian randomization

Abstract

Background The causal effects of plasma lipid concentrations and the risk of primary open angle glaucoma (POAG) are still unclear. Thus, the purpose of this study was to identify, applying a two-sample Mendelian randomization (MR) analysis, whether plasma lipid concentrations are causally associated with the risk of POAG. Methods Two-sample MR analysis of data from a genome-wide association study (GWAS) was performed to investigate the causal role of plasma lipid levels and POAG. A total of 185 independent single-nucleotide polymorphisms (SNPs) associated with plasma lipid levels were selected as instrumental variables (IVs). The SNPs were obtained from a meta-analysis of GWAS based on 188,577 European-ancestry individuals for MR analyses. Association with POAG for the SNPs was obtained from a GWAS conducted among the United Kingdom (UK) Biobank study participants with a total of 463,010 European-ancestry individuals. Four MR methods (inverse variance weighted [IVW], weighted mode, weighted median, and MR-Egger regression) were applied to obtain the overall causal estimate for multiple, instrumental SNPs. Results Using the IVW analysis method, no evidence was found to support a causal association between plasma LDL-C level and POAG risk (β = − 0.00026; 95% CI = -0.00062, 0.00011; P = 0.165) with no significant heterogeneity among SNPs. The overall causal estimate between plasma LDL-C level and POAG was consistent using the other three MR methods. Using the four MR methods, no evidence of an association between plasma HDL-C (β = 0.00023; 95% CI = -0.00015, 0.00061; P = 0.238; IVW method) or TG levels (β = − 0.00028; 95% CI = -0.00071, 0.00015; P = 0.206; IVW method) and POAG risk was found. Sensitivity analyses did not reveal any sign of directional pleiotropy. Conclusions The present study did not find any evidence for a causal association between plasma lipid levels and POAG risk. Further research is needed to elucidate the potential biological mechanisms to provide a reasonable interpretation for these results.

The Role of the ATP-Binding Cassette A1 (ABCA1) in Human Disease

“…Although glaucoma is not a characteristic of Tangier disease, GWAS for IOP and POAG have identified common variants in or near ABCA1 (rs2472493 and rs2487032) among more than 50 loci in Asian and European Caucasian populations [ 292 , 293 , 294 , 295 ]. However, while ABCA1 and other genes involved in lipid metabolism were found to be associated with IOP and POAG, a Mendelian randomization study did not find any evidence for a causal association between plasma lipid levels and POAG risk [ 296 ].…”

Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma

“…Uroporphyrinogen decarboxylase encoded by UROD was an important element in hemoglobin synthesis, which is significantly up-regulated in POAG ( Figure 5D ). Studies have shown that patients with POAG have red blood cell backlog and high plasma specific viscosity ( Wang et al, 2004 ; Xu et al, 2020 ), indicating that the up-regulation of UROD may be an important cause of blood deformation in POAG patients. Further, we found that AL590666.2 and UROD have a strong correlation ( Figure 5E ), suggesting that AL590666.2 may be an important biomarker of POAG.…”

Plasma metabolomics of primary open-angle glaucoma in three prospective US cohorts and the UK Biobank

“…The relation between triglycerides and glaucoma has been conflicting, with studies showing an adverse association, 41 inverse association 42 and null association (Mendelian randomization study 43 ). A systematic review 44 of 12 studies reported a significantly higher risk of open-angle glaucoma with hyperlipidemia as well as hypertriglyceridemia (OR=1.42; 95%CI 1.04, 1.93; based on pooling of 4 studies).…”