Plasma desmoplakin I biomarker of vascular recurrence after ischemic stroke

López‐Farré, Antonio; Zamorano-León, José J.; Segura, Antonio; Mateos-Cáceres, Petra J.; Modrego, Javier; Rodrı́guez-Sierra, Pablo; Calatrava, Laura; Tamargo, Juan; Macaya, Carlos

doi:10.1111/j.1471-4159.2012.07683.x

Cited by 13 publications

(18 citation statements)

References 46 publications

(74 reference statements)

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“…As previously reported in detail, spots from three different 2-DE gels were excised, digested with trypsin and purified as reported 15. Mass spectrometry and MS/MS analysis were performed in a 4700 Proteomic Analyzer (Applied Biosystem, Old Conneticut path, Frammingham, MA, USA) that operated in a reflector positive mode.…”

Section: Methodsmentioning

confidence: 99%

“…As previously reported in detail, for two-dimensional electrophoresis (2-DE) 500 lg of total plasma proteins were used [10,15]. Plasma samples were loaded on immobilized gradient Immobilized pH Gradient (IPG) strips (18 cm, pH 4-7) and isoelectric focusing was performed with a Protean IEF cell system (Bio-Rad, Hercules, CA, USA).…”

Section: Two-dimensional Electrophoresis Of Plasma Proteins Image Acmentioning

confidence: 99%

“…As previously reported, the gels were then fixed, silver stained during 30 min. and scanned in a UMAX POWERLOOK III Scanner [10,15]. One 2-DE gel was performed for each patient and all of the here-identified spots were at least expressed in 70% of the 2-DE gels.…”

Section: Two-dimensional Electrophoresis Of Plasma Proteins Image Acmentioning

confidence: 99%

“…The spots were densitometrically analysed by using the Quantity The protein contained in the spots was identified by using mass spectrometry (MS) and tandem mass spectrometry (MS/MS). As previously reported in detail, spots from three different 2-DE gels were excised, digested with trypsin and purified as reported [15]. Mass spectrometry and MS/MS analysis were performed in a 4700 Proteomic Analyzer (Applied Biosystem, Old Conneticut path, Frammingham, MA, USA) that operated in a reflector positive mode.…”

Section: Two-dimensional Electrophoresis Of Plasma Proteins Image Acmentioning

confidence: 99%

“…Peptides with a signal-to-noise greater than 20 were considered in the Mascot Database for protein identification. To identify the sports, Mascot database 1.9 (http://www.matrixscience.com) was used as an algorithm to match the peptides obtained by MS. As previously reported [15], identifications were accepted based on a tripartite evaluation that takes into account significant molecular weight search (Mowse) scores, spectrum annotation and observed versus expected migration on the 2-DE gel. Each spot was identified twice and only plasma proteins and plasma protein isotypes not previously published identified by MS by our group were identified here.…”

Section: Two-dimensional Electrophoresis Of Plasma Proteins Image Acmentioning

confidence: 99%

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New circulating biomarkers for predicting cardiovascular death in healthy population

Melander

Modrego

Zamorano-León

et al. 2015

J Cellular Molecular Medi

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There is interest to analyse newer biomarkers to identify healthy individuals at risk to develop cardiovascular disease (CVD) incidents and death. To determine in healthy individuals new circulating protein biomarkers, whose systemic levels may be associated with the risk of future development of CVD incidents and death. The study was performed in 82 individuals from the Malmö Diet and Cancer study cohort, free from CVD of whom 41 developed CVD and 41 did not. Plasma proteins related to inflammation and thrombo-coagulating processes were analysed. α1-antitrypsin isotype 3 plasma levels were significantly higher while apolipoprotein J plasma levels were lower in participants that developed CVD incidents than those that did not develop acute cardiovascular episode. Of 82 participants, 17 died by CVD causes. There were proteins whose expression in plasma was significantly higher in participants suffering CVD death as compared with those that did not die by CVD. These proteins included: fibrinogen β-chain isotypes 1 and 3, fibrinogen-γ-chain isotype 2, vitamin D-binding protein isotypes 1, 2 and 3, α1-antitrypsin isotypes 3 and 6, haptoglobin isotypes 3,4,5 and 5, haemopexin isotypes 1 and 2, and Rho/Rac guanine nucleotide exchange factor 2. Moreover, apolipoprotein J plasma levels were found lower in participants that died by cardiovascular cause. Association between plasma levels of proteins and CVD death was independent of age, gender, conventional risk factors and plasma C-reactive protein levels. Several protein plasma levels and protein isotypes related to inflammation and thrombo-coagulating phenomena were independently associated with the risk of future CVD death.

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Section: Methodsmentioning

confidence: 99%

Section: Two-dimensional Electrophoresis Of Plasma Proteins Image Acmentioning

confidence: 99%

Section: Two-dimensional Electrophoresis Of Plasma Proteins Image Acmentioning

confidence: 99%

Section: Two-dimensional Electrophoresis Of Plasma Proteins Image Acmentioning

confidence: 99%

Section: Two-dimensional Electrophoresis Of Plasma Proteins Image Acmentioning

confidence: 99%

See 3 more Smart Citations

New circulating biomarkers for predicting cardiovascular death in healthy population

Melander

Modrego

Zamorano-León

et al. 2015

J Cellular Molecular Medi

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Superswelling Microneedle Arrays for Dermal Interstitial Fluid (Prote)Omics

Laszlo

Crescenzo

Nieto-Argüello

et al. 2021

Adv Funct Materials

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The noninvasive sampling of dermal interstitial fluid (ISF) for the monitoring of clinical biomarkers is a greatly appealing area of research. The identification of molecular biomarkers in biological fluids has been accelerated with ‐omics analyses but remains limited in ISF because of its time‐consuming and complex extraction process. Here, the generation of microneedle (MN) patches made of superabsorbent acrylate‐based hydrogels for the rapid sampling of dermal ISF is described to explore its proteome. In depth, iterative optimization allows the identification of novel acrylate‐based compositions with the required chemical, mechanical, and biocompatibility properties allowing proteomic analysis of the extracted ISF for the first time after sampling with swelling MNs. The generated MN arrays show no cytotoxic effect, successfully cross the stratum corneum, and can collect up to 6 µL of dermal ISF in 10 min in vivo. Proteomics lead to the detection of 176 clinically relevant biomarkers in the collected samples validating the use of ISF as a relevant bodily fluid for disease monitoring and diagnostic. Importantly, it is discovered that extraction fingerprint is strongly dependent on the MNs chemistry, and thus specific biomarkers could be selectively extracted by tuning the composition of the patch, making the system versatile and specific.

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Distinct proteomic profiles in monozygotic twins discordant for ischaemic stroke

et al. 2019

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Background and Purpose: Stroke is a common disorder with significant morbidity and mortality, and complex aetiology involving both environmental and genetic risk factors. Although some of the major risk factors for stoke, such as smoking and hypertension, are welldocumented, the underlying genetic and detailed molecular mechanisms remain elusive. Exploring the relevant biochemical pathways may contribute to the clinical diagnosis of stroke and shed light on its aetiology. Methods: A comparative proteomic analysis of blood serum of a pair of monozygotic (MZ) twins discordant for ischaemic stroke (IS) was performed using a label-free quantitative proteomics approach. To overcome the limit of reproducibility in the serum preparation, two separate runs were performed, each consisting of three technical replicates per sample. Biological processes associated with proteins differentially expressed between the twins were explored with Gene Ontology (GO) classification using the functional analysis tool g:Profiler. Results: ANOVA test performed in Progenesis LC-MS identified 179 (run 1) and 209 (run 2) proteins as differentially expressed between the affected and unaffected twin (P<0.05). Furthermore, the level of serum fibulin 1, an extracellular matrix protein associated with arterial stiffness, was on average 13.37-fold higher in the affected twin. Each dataset was then analysed independently, and the proteins were classified according to GO terms. The categories overrepresented in the affected twin predominantly corresponded to stroke-relevant processes, including wound healing, blood coagulation and haemostasis, with a high proportion of the proteins overexpressed in the affected twin associated with these terms. By contrast, in the unaffected twin diagnosed with atopic dermatitis, there were increased levels of keratin proteins and GO terms associated with skin development. 3 Conclusion: The identification of cellular pathways enriched in IS as well as the up-regulation of fibulin 1 sheds new light on the underlying disease-causing mechanisms at the molecular level. Our findings of distinct proteomic signatures associated with IS and atopic dermatitis suggest proteomic profiling could be used as a general approach for improved diagnostic, prognostic and therapeutic strategies.

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Plasma desmoplakin I biomarker of vascular recurrence after ischemic stroke

Cited by 13 publications

References 46 publications

New circulating biomarkers for predicting cardiovascular death in healthy population

New circulating biomarkers for predicting cardiovascular death in healthy population

Superswelling Microneedle Arrays for Dermal Interstitial Fluid (Prote)Omics

Distinct proteomic profiles in monozygotic twins discordant for ischaemic stroke

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