Plant Tissue Culture Procedure - Background

George, Edwin F.; Hall, Michael; Klerk, G.J.M. de

doi:10.1007/978-1-4020-5005-3_1

Cited by 261 publications

(317 citation statements)

References 89 publications

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“…2). Yang et al 2010;Huang et al 2011;Sharma et al 2012;Sharma et al 2014 also reported better shoot regeneration response by using combinations of BAP and NAA. Qin et al (2006) found that cotyledon was more responsive as compared to hypocotyl explants, whereas Ravanfar et al (2009) found that hypocotyl and shoot tips were promising explants for shoot regeneration in broccoli.…”

Section: Resultsmentioning

confidence: 98%

“…Qin et al (2006) found that cotyledon was more responsive as compared to hypocotyl explants, whereas Ravanfar et al (2009) found that hypocotyl and shoot tips were promising explants for shoot regeneration in broccoli. According to George et al (2008), BAP promotes differentiation of cells into adventitious buds followed by the formation of shoots. The regenerated shoots elongated on the same medium.…”

Section: Resultsmentioning

confidence: 99%

“…The data recorded for the different parameters were subjected to BComplete Randomized Design^. The statistical analysis based on mean values per treatment was made using analysis of variance of CRD (Gomez and Gomez 1984).…”

Section: Discussionmentioning

confidence: 99%

“…Development of high frequency plant regeneration protocol is a pre-requisite to carry out genetic transformation studies. Plant regeneration studies in broccoli were carried out by various workers using various explants such as peduncle (Christey and Earle 1991), hypocotyl (Puddephat et al 2001;Kim and Botella 2002;Ravanfar et al 2009), leaf tissue (Robertson and Earle 1986;Cao and Earle 2003) and cotyledon (Qin et al 2006;Huang et al 2011). In this paper, we described the high frequency shoot regeneration ability of hypocotyl, cotyledon, leaf and petiole explants by using different combination of BAP and NAA in broccoli (Brassica oleracea L. var.…”

Section: Introductionmentioning

confidence: 96%

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High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

Kumar

Srivastava

2015

Physiol Mol Biol Plants

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Broccoli (Brassica oleracea L. var. italica) is an important, nutritionally rich vegetable crop, but severely affected by environmental stresses, pests and diseases which cause massive yield and quality losses. Genetic manipulation is becoming an important method for broccoli improvement. In the present study, a reproducible and highly efficient protocol for obtaining organogenesis from hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica cv. Solan green head) has been developed. Hypocotyl and cotyledon explants were used from 10 to 12 days old aseptically grown seedlings whereas leaf and petiole explants were excised from 18 to 20 days old green house grown seedlings and surface sterilized. These explants were cultured on shoot induction medium containing different concentration and combination of BAP and NAA. High efficiency shoot regeneration has been achieved in hypocotyl (83.33 %), cotyledon (90.11 %), leaf (62.96 %) and petiole (91.10 %) explants on MS medium supplemented with 3.5 mg/l BAP+ 0.019 mg/l NAA 2.5 mg/l BAP+0.5 mg/l NAA, 4.0 mg/l BAP+0.5 mg/l NAA and 4.5 mg/l BAP+0.019 mg/l NAA respectively. Petiole explants showed maximum shoot regeneration response as compared to other explants. MS medium supplemented with 0.10 mg/l NAA was found best for root regeneration (100 %) from in vitro developed shoots. The regenerated complete plantlets were transferred to the pots containing cocopeat and successfully acclimatized. This optimized regeneration protocol can be efficiently used for genetic transformation in broccoli. This is the first comparative report on multiple shoot induction using four different types of explants viz. hypocotyl, cotyledon, leaf and petiole.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

Kumar

Srivastava

2015

Physiol Mol Biol Plants

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“…57 In the sense of plants propagation, plant in vitro cultures present an opportunity to obtain a large number of new plants from just one source of material in a very short period of time. 58 This method is especially valid for plants with industrial importance, such as: ornamental, fruited, or forestry ones. It is used for elite plants with high unitary value as well.…”

Section: Plant In Vitro Morphogenesismentioning

confidence: 99%

Considerations on Legal and Philosophical Problems in Experimental Botany: The case of plant in vitro cultures

Kleszcz

Jakubiec

2015

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The present paper consists of two parts. In the first, some issues related to the character of biological experiments conducted under in vitro cultures are portrayed. The relevant aspects of these procedures are explicated from the viewpoint of the experimental botanist. It is a case study for the considerations in the second part, which presents selected philosophical and legal issues involved in biological experiments from the general perspective of philosophical investigations concerning the problem of plants’ axiology. Obviously, the nature of the considerations is limited; not all important questions from the perspective of biology and philosophy have been raised. Nevertheless, the authors hope that the analyzed issues may be interesting for both biologists and philosophers.

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Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy

et al. 2019

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In the present study a new luminescent dye 3‐N‐(2‐pyrrolidinylacetamido)benzanthrone (AZR) was synthesized. Spectroscopic measurements of the novel benzanthrone 3‐aminoderivative were performed in seven organic solvents showing strong fluorescence. The capability of the prepared dye for visualization has been tested on flax, red clover and alfalfa to determinate the embryo in plant callus tissue cultures. Callus cells were stained with AZR and further analysed utilizing confocal laser scanning fluorescence microscopy. Performed experiments show high visualization effectiveness of newly synthesized fluorescent dye AZR that is efficient in fast and relatively inexpensive diagnostics of callus embryos that are problematic due to in vitro culture specificity.

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Plant Tissue Culture Procedure - Background

Cited by 261 publications

References 89 publications

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

Considerations on Legal and Philosophical Problems in Experimental Botany: The case of plant in vitro cultures

Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy

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